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Method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells

A bone marrow mesenchymal, islet-like cell technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve problems such as unsatisfactory effects, islet-like cell defects, and low insulin secretion, and achieve excellent results. , the effect of large amount of islet-like cells

Inactive Publication Date: 2009-12-16
NORTHWEST A & F UNIV
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Problems solved by technology

[0004] The above facts prove that: currently there are many methods for inducing hMSCs to differentiate into islet-like cells in vitro, but the insulin secretion of the obtained islet-like cells is low, and the effect is not ideal when applied. The method is flawed

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  • Method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells
  • Method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells
  • Method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells

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Embodiment Construction

[0028] The invention discloses a new four-step induction method composed of multiple inducers, in which fetal pig pancreas extract and zinc acetate are the induction components used for the first time. This method can induce hMSCs to differentiate into islet-like cells whose insulin secretion is higher than that reported in the literature, and transplant them into STZ-induced diabetes model nude mice. %. All nude mice in the control group died.

[0029] The present invention specifically includes the following contents:

[0030] A new system composed of various inducers was used to induce hMSCs to differentiate into islet cells in vitro: hMSCs were cultured until 70% of the bottom of the dish was covered, and the following 4 steps were used in sequence at 37°C and 5% CO 2 Induction was carried out in the incubator, and the medium was changed every 2 days for a total of 10-12 days.

[0031] Step 1: Induce for 1 hour with high-glucose DMEM containing 4 mmol / L β-mercaptoethano...

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Abstract

The invention relates to a method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells, comprising four inducing methods for dividing the human medulla mesenchymal stem cells into islet-like cells, wherein the main inducing liquid firstly adopts porcine fetal pancreas extracting solution and the inducing ingredients of zinc acetate, and the secretory volume of insulin which is divided into the islet-like cells is remarkably improved. The islet-like cells obtained by the method are possible to break through the current technical problem of diabetes mellitus and are suitable for experimental research and future large-scale production.

Description

technical field [0001] The invention relates to a method for cell differentiation to obtain islet-like cells, in particular to a method for inducing human bone marrow mesenchymal stem cells to differentiate into islet-like cells in vitro. Background technique [0002] Diabetes has become the third major disease that endangers human health after cardiovascular disease and tumors. At present, islet transplantation is a better treatment method for this disease, but this method is hindered by the lack of islet sources and immune rejection between allogeneic. Human bone marrow mesenchymal stem cells (hMSCs) can be derived from diabetic patients. They are convenient to obtain, and are easy to isolate, culture and expand in vitro. If hMSCs are induced to differentiate into islet-like cells, the problem of islet cell origin and immune exclusion can be completely solved. It is expected to realize the treatment of diabetes with autologous cells. [0003] At present, research on induc...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 张翊华窦忠英
Owner NORTHWEST A & F UNIV
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