Method for inducing in vitro human medulla mesenchymal stem cells to be divided into islet-like cells
A bone marrow mesenchymal, islet-like cell technology, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve problems such as unsatisfactory effects, islet-like cell defects, and low insulin secretion, and achieve excellent results. , the effect of large amount of islet-like cells
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[0028] The present invention discloses a four-step induction method composed of multiple inducers, in which fetal pig pancreas extract and zinc acetate are the first induction components used. This method can induce hMSCs to differentiate into islet-like cells with higher insulin secretion than reported in the literature, and transplant them into STZ-induced diabetes model nude mice. The blood glucose level of the nude mice is completely restored to normal levels, and the survival rate of nude mice reaches 100. %. All nude mice in the control group died.
[0029] The present invention specifically includes the following contents:
[0030] A new system composed of a variety of inducers was used to induce hMSCs to differentiate into pancreatic islet cells in vitro: culture hMSCs to 70% full plate bottom, followed by the following 4 steps at 37°C, 5% CO 2 The induction is carried out in the incubator, and the medium is changed every 2 days for a total of 10-12 days.
[0031] Step 1: ...
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