Quality control method for Keyin pills

Abstract

The invention relates to a quality control method for Keyin pills. Through the thin-layer identification of asiatic moonseed rhizome, densefruit pittany root-bark and bistort rhizome in the Keyin pills and the detection of n-butanol extract, the quality control method effectively controls the quality of medicaments, has the characteristics of stability, rapidity, sensitivity and reliability, and can comprehensively reflect the efficacy of the medicaments.

Description

technical field

[0001] The invention relates to a quality control method of Keyin pills, belonging to the technical field of traditional Chinese medicines. Background technique

[0002] Keyin Wan has the effect of clearing away heat and detoxification, dispelling wind and relieving itching, and is used for psoriasis with red base of skin lesions, red base of tongue, constipation, and yellow urine belonging to blood heat and wind dryness type. The national standard "Prescriptions of Traditional Chinese Medicine" Volume 6, page 69, discloses the quality standard of Keyin pills:

[0003] [Prescription] Smilax cocos 30g, white fresh skin 30g

[0004] Northern Bean Root 10g Box Ginseng 30g

[0005] [Preparation method] Mix the above four flavors, take 15g and grind into fine powder, sieve, and mix well. The remaining 85g was decocted with water three times, for the first time for 2 hours, for the second time for 1 hour, for the third time for 1 hour, the decoction was combined...

Examples

experiment example 1

[0026] Experimental Example 1 Qualitative detection experiment of bean root

[0027] 1. The investigation of the preparation method of the test solution

[0028] Take the pill obtained in Example 2, pulverize it, weigh 5 parts, 10 grams per part, add 25 ml of chloroform, 1 ml of concentrated ammonia solution, heat and reflux on a water bath for 20 min, 40 min, 60 min, 80 min, 100 min, filter, and evaporate the filtrate to dryness. Add 1ml of chloroform to the residue to dissolve it as the test solution. Another 1 g of Beidougen reference medicinal material was taken, and the same method was used to prepare the reference medicinal material solution respectively. According to the thin-layer chromatography test, absorb the same amount of the above-mentioned solution, spot it on the same silica gel G thin-layer plate, use 9:2 ratio of benzene-acetone as the developing solvent, put it in a chromatography cylinder saturated with ammonia vapor, develop, take out , dry it, and inspe...

experiment example 2

[0045] Experimental example 2 qualitative detection experiment of white fresh bark

[0046] 1. The investigation of the preparation method of the test solution

[0047] Take 1 g of white fresh cortex reference medicinal material, and prepare the reference medicinal material solution according to the preparation method under test a. Take the test solution under item a, test according to the thin-layer chromatography method, draw the above two solutions, respectively point on the same silica gel G thin-layer plate, use benzene-acetone as the developing agent, and set the ammonia vapor saturated chromatography In the cylinder, unfold, take out, dry, and put it under the ultraviolet light (365nm) to inspect, in the chromatogram of the test product, the fluorescent spots of the same color are displayed on the positions corresponding to the chromatogram of the control medicinal material. Known by above-mentioned test, in the detection method a, need testing solution contains the sa...

experiment example 3

[0060] Experimental Example 3 Qualitative detection experiment of gallic acid in ginseng

[0061] 1. The investigation of the preparation method of the test solution

[0062] Take the pill obtained in Example 2, pulverize it, weigh 5 parts, 10 grams per part, add 30 ml of water and stir to make it uniformly dispersed, filter it with absorbent cotton, extract the filtrate twice with the following solvent, 30 ml each time, combine the extracts, and concentrate To 1ml, as the test solution. Take gallic acid reference substance, add ethanol to make a solution containing 0.5mg per 1ml, as the reference substance solution. According to the thin-layer chromatography test, absorb 10 μl of the above-mentioned equal amount of the test solution and the reference solution, respectively spot on the same silica gel G thin-layer plate, use toluene-ethyl acetate-formic acid as the developer, develop, take out, cool Dry, spray 1% ferric chloride ethanol solution, in the chromatogram of the t...

technical field

[0001] The invention relates to a quality control method of Keyin pills, belonging to the technical field of traditional Chinese medicines. Background technique

[0002] Keyin Wan has the effect of clearing away heat and detoxification, dispelling wind and relieving itching, and is used for psoriasis with red base of skin lesions, red base of tongue, constipation, and yellow urine belonging to blood heat and wind dryness type. The national standard "Prescriptions of Traditional Chinese Medicine" Volume 6, page 69, discloses the quality standard of Keyin pills:

[0003] [Prescription] Smilax cocos 30g, white fresh skin 30g

[0004] Northern Bean Root 10g Box Ginseng 30g

[0005] [Preparation method] Mix the above four flavors, take 15g and grind into fine powder, sieve, and mix well. The remaining 85g was decocted with water three times, for the first time for 2 hours, for the second time for 1 hour, for the third time for 1 hour, the decoction was combined...

Examples

experiment example 1

[0026] Experimental Example 1 Qualitative detection experiment of bean root

[0027] 1. The investigation of the preparation method of the test solution

[0028] Take the pill obtained in Example 2, pulverize it, weigh 5 parts, 10 grams per part, add 25 ml of chloroform, 1 ml of concentrated ammonia solution, heat and reflux on a water bath for 20 min, 40 min, 60 min, 80 min, 100 min, filter, and evaporate the filtrate to dryness. Add 1ml of chloroform to the residue to dissolve it as the test solution. Another 1 g of Beidougen reference medicinal material was taken, and the same method was used to prepare the reference medicinal material solution respectively. According to the thin-layer chromatography test, absorb the same amount of the above-mentioned solution, spot it on the same silica gel G thin-layer plate, use 9:2 ratio of benzene-acetone as the developing solvent, put it in a chromatography cylinder saturated with ammonia vapor, develop, take out , dry it, and inspe...

experiment example 2

[0045] Experimental example 2 qualitative detection experiment of white fresh bark

[0046] 1. The investigation of the preparation method of the test solution

[0047] Take 1 g of white fresh cortex reference medicinal material, and prepare the reference medicinal material solution according to the preparation method under test a. Take the test solution under item a, test according to the thin-layer chromatography method, draw the above two solutions, respectively point on the same silica gel G thin-layer plate, use benzene-acetone as the developing agent, and set the ammonia vapor saturated chromatography In the cylinder, unfold, take out, dry, and put it under the ultraviolet light (365nm) to inspect, in the chromatogram of the test product, the fluorescent spots of the same color are displayed on the positions corresponding to the chromatogram of the control medicinal material. Known by above-mentioned test, in the detection method a, need testing solution contains the sa...

experiment example 3

[0060] Experimental Example 3 Qualitative detection experiment of gallic acid in ginseng

[0061] 1. The investigation of the preparation method of the test solution

[0062] Take the pill obtained in Example 2, pulverize it, weigh 5 parts, 10 grams per part, add 30 ml of water and stir to make it uniformly dispersed, filter it with absorbent cotton, extract the filtrate twice with the following solvent, 30 ml each time, combine the extracts, and concentrate To 1ml, as the test solution. Take gallic acid reference substance, add ethanol to make a solution containing 0.5mg per 1ml, as the reference substance solution. According to the thin-layer chromatography test, absorb 10 μl of the above-mentioned equal amount of the test solution and the reference solution, respectively spot on the same silica gel G thin-layer plate, use toluene-ethyl acetate-formic acid as the developer, develop, take out, cool Dry, spray 1% ferric chloride ethanol solution, in the chromatogram of the t...