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Antigenic epitope of human tumor necrosis factor alpha and preparation method and application thereof
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A tumor necrosis factor and antigen epitope technology, which is applied in the fields of protein engineering, molecular biology and immunology, and can solve problems such as antigen epitopes without tumor necrosis factor alpha.
Active Publication Date: 2013-08-14
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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With the deepening of research on antigenic epitopes, the need to obtain effective tumor necrosis factor-α antigenic epitopes is also increasing day by day, but so far there is no relevant report on obtaining effective tumor necrosis factor-α antigenic epitopes
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[0081] Ph.D.-12 Peptide Library Kit was purchased from NEB Company, with a library capacity of 2.7x10 9 , with a titer of 1.5x10 13 / μl. Escherichia coli ER2537 is the host strain of the peptide library. For the screening process, refer to the instruction manual of the phage display peptide library. Each round of screening was coated with anti-rhTNFα antibody (10 μg per well), and 1×10 11 Phage. Phage enrichment was calculated by the anti-in / out ratio. After three rounds of screening, positive clones were selected for sequencing. The specific binding of positive phage clones to anti-rhTNFα antibody was determined by ELISA. Anti-rhTNFα antibody (10 μg per well) was coated on a 96-well plate (Nunc Company) overnight at 4°C. Unbound antibodies were poured off and blocked with 3% BSA at 37°C for 1 hour. Put 1x10 in each hole 9 Phage were incubated at 37°C for 2 hours. The washi...
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Abstract
The invention relates to the fields of molecular biology and immunology, in particular to an antigenic epitope of a human tumor necrosis factor alpha, which has the following amino acid sequence shown in a general formula (I): A1A2A3A4A5A6A7A8A9A10A11A12, wherein A1, A2 and A6 refer to amino acid S; A5 and A9 refer to amino acid P, A3, A4, A7, A8, A10, A11 and A12 respectively refer to amino acid R, T, D, K, V, A and H; and the amino acid sequence shown in the general formula (I) is not substituted or substituted, and the substitution means that one or more of A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A11 and A12 are optionally substituted by amino acid A. The invention also provides a preparation method and application of the antigenic epitope.
Description
technical field [0001] The invention relates to the fields of protein engineering, molecular biology and immunology, in particular, the invention relates to a novel human tumor necrosis factor alpha epitope and its preparation method and application. Background technique [0002] Known tumor necrosis factor α (TNF-α) is a cytokine with a variety of biological activities, mainly derived from monocytes and macrophages, T lymphocytes, neutrophils, mast cells and endothelial cells in It can also be produced under certain conditions [Old L (1985) Science230: 630-632]. Mature TNF-α has a molecular weight of 17kD and binds to the TNF-α receptor (TNFR) on the cell surface in the form of a trimer to mediate various biological activities. Normal levels of TNF-α can participate in resisting bacterial, viral and parasitic infections, promoting tissue repair, and causing tumor cell apoptosis, but the large amount of TNF-α produced and released in the body will destroy the immune balance...
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