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Gonorrhea-antigen spittle rapid detection strip

A technology for detecting test strips and antigens, applied in the field of biological diagnosis, can solve the problems that test strips are not suitable for on-site testing and high testing costs, and achieve the effects of being suitable for on-site testing and self-testing, high sensitivity, and simple operation

Inactive Publication Date: 2010-03-03
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a test strip for rapid detection of gonorrhea antigen saliva, the test strip can use saliva as a sample, which solves the problem that the existing test strip is not suitable for on-site detection and the detection cost is high

Method used

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  • Gonorrhea-antigen spittle rapid detection strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of colloidal gold pads

[0025] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 0.5 mg of the first monoclonal antibody that can specifically bind to the gonorrhea antigen (mouse anti-human Shanghai Lingchao Biotechnology Co., Ltd.), stir at room temperature for 1 hour, and add 5% bovine serum white Protein (BSA) was blocked for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0026] Coating of nitrocellulose membrane

[0027] The second monoclonal antibody (mouse anti-human Shanghai Lingchao Biotechnology Co., Ltd.) that c...

Embodiment 2

[0033] Preparation of colloidal gold pads

[0034] Prepare a colloidal gold solution with a diameter of 35nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.2, add 1.0 mg of the first monoclonal antibody (mouse anti-human Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the gonorrhea antigen, stir at room temperature for 1 hour, and add 5% bovine serum white Protein (BSA) was blocked for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0035] Coating of nitrocellulose membrane

[0036] The second monoclonal antibody (mouse anti-human Shanghai Lingchao Biotechnology Co., Ltd.) that c...

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Abstract

The invention discloses a gonorrhea-antigen spittle rapid detection strip comprising a baseboard, to which a sample pad, a colloidal gold pad, a nitrocellulose membrane and a sample-drawing pad are sequentially adhered, wherein a first monoclonal antibody which is marked by colloidal gold and can be integrated with the specificity of the gonorrhea-antigen is attached to the colloidal gold pad; thenitrocellulose membrane is coated with a detection line formed by a second monoclonal antibody and a quality control line formed by an antiantibody which can be integrated with the specificity of thefirst monoclonal antibody; and the first monoclonal antibody is matched with the second monoclonal antibody. The gonorrhea-antigen spittle rapid detection strip provided by the invention adopts the colloidal gold marking technology to detect the gonorrhea-antigen contained in spittle so as to determine whether the person is infected with gonococcus, and has the advantages that operation is simple; reaction is quick; sensitivity is high; specificity is strong; and the gonorrhea-antigen spittle rapid detection strip can be applied to both on-site detection and self-detection, and is economicaland practical and the like.

Description

technical field [0001] The invention relates to the technical field of biological diagnosis, in particular to a gonorrhea antigen saliva rapid detection test strip. Background technique [0002] Gonorrhea (gonorrhea) is a sexually transmitted disease caused by Neisseria gonorrhoeae (abbreviated as gonorrhea) and is mainly manifested by purulent infection of the genitourinary system. It is an ancient and common sexually transmitted disease. In recent years, the incidence rate ranks the first among sexually transmitted diseases in my country (China). Gonorrhea is a Gram-negative diplococcus, which is kidney-shaped and arranged in pairs. It is difficult to survive without the human body. It is easy to kill it with general disinfectants, and it mostly occurs in young men and women. . [0003] Before liberation, the incidence of gonorrhea in some cities in my country was about 20%. After liberation, early patients were almost extinct in 1953, and the census and general treatment...

Claims

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Application Information

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IPC IPC(8): G01N33/571G01N33/577G01N33/558G01N33/532G01N33/52
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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