Animal feed additive
A feed additive and technology for animals, applied in animal feed, animal feed, application, etc., can solve problems that have not been ingested, have not been studied, and have not been studied by oral administration to animals
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[0172] Cultivation of Aspergillus
[0173] Adjust the pH of the potato dextrose agar medium to 5 and sterilize at 121°C for 15 minutes. When the temperature of the agar medium dropped to 60°C, sodium deoxycholate was added at a concentration of 10 g per 1 L of the medium to inoculate the Aspergillus stored in Akita Konno Co., Ltd. (248 Karwano, Daisen City, Akita Prefecture, Japan). As a result, Aspergillus sojae, Aspergillus tamarii, Aspergillus foetidus, and Aspergillus niger reproduced well in the medium. In particular, the Aspergillus sojae AOK210 strain, the Aspergillus slippery AOK43 strain, the Aspergillus foetidus AOK N4586 strain, and the Aspergillus niger AOK B650 strain reproduced well.
[0174] In the same medium containing sodium deoxycholate as described above, various fermented foods are used as separation sources to inoculate many Aspergillus spp. strains, and the best breeding strains are selected from the strains propagated in the medium. This strain is designa...
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