Method for measuring content of phospholipid in liposome
A technology of phospholipid content and determination method, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of unreasonable ratio of color reagent solution, complicated operation steps, difficult temperature control, etc., and achieve display effect Good, reduce environmental pollution, improve the effect of accuracy
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[0047] Adopt method of the present invention to carry out liposome content determination to commercially available amphotericin B liposome for injection
[0048] (1) Tested sample:
[0049] Amphotericin B liposome for injection (Amphotec) 50mg: 12ml
[0050] Manufacturer: Ben Venue Laboratorics.Inc.USA
[0051] Production batch number: 141087E, 141088E, 141089E
[0052] (2) Determination method:
[0053] a. Preparation of chromogenic agent
[0054] A Chromogenic Agent: Accurately weigh 0.22g of ammonium molybdate, dissolve it in distilled water and pour it into a 100ml volumetric flask, then slowly add 4.72ml of analytically pure sulfuric acid, let it cool, dilute to the mark, and pour it into a brown bottle Store in the refrigerator;
[0055] B Chromogen: Accurately weigh 15.00 g of L-ascorbic acid in a beaker, and use 14% Na 2 SO 3 Dissolve and dilute to a 100ml volumetric flask, pour into a brown bottle and store in the refrigerator;
[0056] b. Preparation of phospho...
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