Method for preparing live lactobacillus planterum preparation and application of live lactobacillus planterum preparation in preventing and treating piglet diarrhoea
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A technology of Lactobacillus plantarum and live bacteria preparation, applied in the field of biotechnology, can solve problems such as heavy research workload, and achieve the effects of alleviating bacterial diarrhea, convenient operation and promoting digestion
Inactive Publication Date: 2010-05-12
许民强
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Embodiment 1
[0034] Two live bacterial preparations of Lactobacillus plantarum are used to treat diarrhea in piglets:
[0035] (1) Activated strains;
[0036]Take two strains of Lactobacillus plantarum, Lactobacillus plantarum CGMCC 1.0557, Lactobacillus plantrums SMB-2CGMCC No 2566 China General Microorganism Culture Collection Center (CGMCC).
[0037] The MRS medium was used, the culture time for slant activation was 48 hours, and the culture temperature was 30±2°C.
[0038] (2) Scale-up culture, with the mother bacteria of activation, adopt liquid fermentation in Erlenmeyer flask; The time of enlargement culture in Erlenmeyer flask is 48 hours, culture temperature 30 ± 2 ℃, medium formula is:
[0039]
[0040] (3) The production culture time is 48 hours, the culture temperature is 30±2°C, and the culture medium formula is:
[0041] soybean meal
50
g
corn
33
g
bran
17
g
pure water
25
L
PH value
6.8
...
Embodiment 2
[0051] A live bacterial preparation of Lactobacillus plantarum is used to treat diarrhea in piglets:
[0052] (1) Activated strains:
[0053] Take two strains of Lactobacillus plantarum, Lactobacillus plantarum CGMCC 1.0557. Using MRS medium, the slant activation culture time is 48 hours, the culture temperature is 30±2℃,
[0054] (2) Scale-up culture, with the mother bacteria of activation, adopt liquid fermentation in Erlenmeyer flask; The time of enlargement culture in Erlenmeyer flask is 48 hours, culture temperature 30 ± 2 ℃, medium formula is:
[0055]
[0056] (3) The production culture time is 48 hours, the culture temperature is 30±2°C, and the culture medium formula is:
[0057] soybean meal
50
g
corn
33
g
bran
17
g
pure water
25
L
PH value
6.8
[0058] (4) making fungus product, its method is:
[0059] After the production liquid is fermented, take the supernatant, pa...
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Abstract
The invention discloses a method for preparing a live lactobacillus planterum preparation and application of the live lactobacillus planterum preparation in preventing and treating piglet diarrhea. The preparation method is characterized in that: one or more lactobacillus planetaria are prepared into a single or composite live bacterium preparation, namely liquid bacterium and dry powdered bacterium, through a set of practical fermentation culture process. The quality requirements on the product are that: the liquid bacterium has a viable count CFU which is more than 108 / ml and is a transparent light yellow liquid of which the pH value is between 2.5 and 4.5; and the dry powdered bacterium has a viable count CFU which is more than 108 / g and is powder which can pass a 60-mesh sieve in 100 percent. When a piglet has bacterial diarrhea during a post-weaning period or other periods, 0.01 to 2 percent of liquid bacterium is added into the drinking water for the piglet and 0.01 to 2 percent of powdered bacterium is added into a feed for the piglet, wherein the dosage is increased or decreased according to the severity of the symptom and effective live bacterium content of the product. Bacterial diarrhea and diarrhoea caused by maldigestion can be effectively relieved or treated by feeding the piglet with the water and the feed.
Description
technical field [0001] The invention relates to the technical field of biotechnology, and more specifically relates to the application of microbial technology in animal production. Background technique [0002] At present, piglet diarrhea is a common disease in pig production, and it is also one of the most critical links that affect the development of pig production and operating efficiency. At present, the common prevention and control measures in production mainly include antibiotics, antibacterial natural substances, including traditional Chinese medicine additives, high copper technology, prebiotics and other technologies. [0003] Antibiotics are the most frequently used technical measures. Long-term use of antibiotics in feed has serious consequences, which is increasingly recognized by the industry and society. 1. Bacteria develop drug resistance; 2. Drug residues in animals or in meat, eggs, and milk; 3. Harm to human health through the food chain; 4. Serious envir...
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