Method for measuring beta-hydroxybutyric acid by using enzyme colorimetric reaction, matched kit and application thereof

A technology of colorimetric reaction and oxybutyric acid, which is applied in the field of medical inspection and measurement, can solve problems such as external interference, and achieve the effects of short reaction time, prevention of complications, and simple operation
CN101726463BActive Publication Date: 2011-10-05NINGBO RUI BIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NINGBO RUI BIO TECH
Publication Date
2011-10-05

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Abstract

The invention provides a method for measuring beta-hydroxybutyric acid by using enzyme colorimetric reaction, a matched kit and application thereof. Under the system of Tris-HCL buffer solution, serum beta-hydroxybutyric acid and coenzyme I are dehydrogenized under the catalysis of beta-hydroxybutyric acid dehydrogenase to generate acetoacetic acid and reduced coenzyme I, the generated reduced coenzyme I and iodonitrotetrazole chloride are reacted under the catalysis of diaphorase to generate a red substance formazane of which the highest absorbance is 505 nanometers, and then the content of the beta-hydroxybutyric acid in a biological sample is quantified by measuring the change of the absorbance of the red product at the wavelength of 505 nanometers. The method can linearly measure the concentration range (0 to 4.5 mmol / L) of the beta-hydroxybutyric acid in the biological sample, the measurement is used for judging the human ketosis for acid poisoning diagnosis, the reagent used by the method is liquid dual-reagent, and the quantity of the sample required for measuring the beta-hydroxybutyric acid is little; moreover, the reaction time during measuring is short, the operation is simple, and the method is suitable for mass detection.
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Description

technical field

[0001] The invention belongs to the technical field of medical examination and measurement, and in particular relates to a method for measuring β-hydroxybutyric acid by using an enzyme colorimetric reaction, a matched kit and its application. Background technique

[0002] The current detection method for the determination of serum β-hydroxybutyric acid is the enzyme kinetic method, in the coenzyme I (NAD + ) in the presence of β-hydroxybutyric acid under the action of β-hydroxybutyrate dehydrogenase to generate acetoacetate, reduced coenzyme I (NADH) and hydrogen ions, and the reduced coenzyme I (NADH) produced by the reaction is at 340nm The change of absorbance can indirectly reflect the concentration of β-hydroxybutyric acid in the patient's serum. Its reaction process is as follows:

[0003]

[0004] However, this method is susceptible to exogenous interference. Contents of the invention

[0005] The technical problem to be solved by the present i...

Claims

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