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In-vitro verification method for apple rot disease resistance

A technology for apple rot disease and apple rot pathogens, applied in the field of in vitro identification of apple rot disease resistance, can solve the problems of rapid onset, high virulence, and difficulty in identifying the resistance of apple varieties, and achieve uniform disease incidence and reliable results , the effect of a controllable amount

Active Publication Date: 2010-06-23
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the mycelium contains more toxins and enzymes that degrade the bark cell wall, this inoculation method is highly virulent, and the disease is rapid, and it is difficult to identify the resistance of apple varieties

Method used

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  • In-vitro verification method for apple rot disease resistance
  • In-vitro verification method for apple rot disease resistance
  • In-vitro verification method for apple rot disease resistance

Examples

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Embodiment 1

[0018] Example 1, Identification and Screening of Rot Resistance of Golden Delicious and Hongyu Hybrid Progeny

[0019] 1. Configuration of culture medium

[0020] (1) Preparation of PDA medium

[0021] Recipe: Peeled Potatoes: 200g

[0022] Sucrose (C 12 h 22 o 11 , CHG / T3462-1999, Beijing Chemical Plant): 20g

[0023] Agar (DH0110-1.1, Beijing Dingguo Biotechnology Co., Ltd.): 15-20g

[0024] Distilled water (GB50172-92): 1000ml

[0025] Steps:

[0026] 1) Weigh 200g of peeled potatoes, cut them into small pieces, put them into a beaker, add 800ml of water, boil for 30min, filter out the potatoes and their residues with gauze, and collect the filtrate;

[0027] 2) Add agar and sucrose to the filtrate, heat and melt, and distill the volume to 1000ml with distilled water;

[0028] 3) the solution is divided into Erlenmeyer flasks, sealed and numbered;

[0029] 4) Put the subpackaged solution in a high-pressure steam sterilizer, and sterilize it by high-pressure steam...

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Abstract

The invention discloses an in-vitro verification method for apple rot disease resistance. The method comprises the following steps: (1) culturing conidiophores of Valsa mali Miyabe et Yamada at 20-30 DEG C for 6-24 hours to obtain the bourgeoned conidiophores; (2) inoculating the isolated branch with the bourgeoned conidiophores in step (1), and culturing at 20-30 DEG C under the relative humidity of 60-100%; and after culturing for 5-16 days, taking out the branches, and identifying the resistance. The verification method for apple rot disease resistance uses the cultured spores in the bourgeoning state to infect the branches according to the characteristics of plant disease resistance research development and rot disease occurrence. The invention conforms to the law of infection of Valsa mali Miyabe et Yamada, has the advantages of moderate pathogenicity and uniform disease occurrence. The method can verify the resistance of apple varieties to the rot disease, and has the advantages of convenience, high speed, simple and easy realization, reliable result and the like.

Description

technical field [0001] The invention relates to an in vitro identification method of apple rot disease resistance. Background technique [0002] Apple rot disease, commonly known as rotten skin disease, is one of the important diseases of apple trees in northern my country. The pathogen Valsa mali Miyabe et Yamada is called apple black rot shell fungus, which belongs to the subphylum Ascomycota. The asexual generation is Cytosporamandshurica Miura, which is called ascocyst of apple dry rot, which belongs to the fungus of the subphylum Deuteromycota. The disease has the characteristics of latent infection and continuous outbreaks. Once the disease occurs, there is no cure, causing tree vigor to weaken, branches to die, dead trees, and even gardens to be destroyed. Therefore, it is an important measure to control apple rot disease by identifying germplasm resistant to apple rot and cultivating resistant varieties. At present, the identification of resistance to apple rot dis...

Claims

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Application Information

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IPC IPC(8): G01N33/48C12Q1/02
Inventor 韩振海张新忠刘广华
Owner CHINA AGRI UNIV
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