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Method for rapidly identifying sex of embryonic-period mice

An embryonic, fetal mouse technology, applied in the directions of inoculation and ovulation diagnosis, medical science, surgery, etc., can solve problems such as inapplicability, and achieve the effect of ensuring accuracy, improving experimental efficiency, and saving manpower and material resources.

Inactive Publication Date: 2010-06-30
北京济普霖生物技术有限公司
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

There are also researchers who dissect the gonads and judge the gender based on the internal early development characteristics of the gonads, but this requires long-term practical experience and is not suitable for everyone

Method used

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  • Method for rapidly identifying sex of embryonic-period mice
  • Method for rapidly identifying sex of embryonic-period mice
  • Method for rapidly identifying sex of embryonic-period mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Materials and utensils

[0018] 1. Experimental animals

[0019] Normal wild-type SPF-grade ICR mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and were raised in an SPF-grade experimental mouse room according to the standard, with 12 hours of light and free access to food.

[0020] 2. Drugs

[0021] DPBS is a product of Gibco

[0022] 3. Main instruments and equipment

[0023] 1) Electrophoresis apparatus: DYY-III2 voltage stabilized electrophoresis apparatus, Beijing Liuyi Instrument Factory

[0024] 2) Stereo mirror: Japan NIKON company

[0025] 3) Ultra-clean workbench: Beijing Purification Equipment Factory

[0026] 4) Low temperature centrifuge: Eppendorf company

[0027] 5) Gel imaging system: ALPHA INNOTECH company

[0028] 6)PHS-3C acidity meter: Shanghai Hongyi Instrument Factory

[0029] 7) Automatic sterilizer: Japan SANYO company

[0030] 8) 37 ℃ constant temperature water bath: American LIFESCIENCE compa...

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Abstract

The invention provides a method for rapidly identifying sex of embryonic-period mice. The method comprises the following steps: placing a fetal rat in the embryonic period of 14.5 to 16 days under a stereoscope; observing a fourth and fifth pair of breasts of the fetal rat; and judging the fetal rat to be male if breast primordia appear to be white round points or judging the fetal rat to be female if the breast primordia appear to be round halos fuzzy in skin boundary. PCR verification shows that the method is reliable to judge the sex of the fetal rat through the appearance of the breast primordial, and the accuracy of the method can be up to 100 percent particularly in the embryonic period of 15 to 16 days. The method has the advantages of providing scientific research personnel with great convenience, greatly improving experiment efficiency, ensuring experiment accuracy and saving manpower and material resources.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying the sex of embryonic mice, in particular to a method for identifying the sex of embryonic days 14.5 (E14.5d) to 16 days (E16d). Background technique [0002] As an ideal model of experimental animals, mice are widely used in scientific research fields such as medicine, biology, and agriculture. When the mouse develops to the embryonic stage (not yet born) about 14.5 days, it is the peak period of gonadal hormone secretion, and it is also the peak period of nervous system development. During this period, many scientific research materials are used for the research of tissues and organs related to the regulation of gonadal hormones. It is a critical period for research on signaling pathways related to gonadal hormones. Therefore, the rapid identification of the sex of fetal mice during this period is of great significance for scientific research. [0003] At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/00
Inventor 宋嘉哲李宁
Owner 北京济普霖生物技术有限公司
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