Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application

An angiogenesis and extract technology, applied in skin care preparations, applications, food preparation and other directions, can solve the problems such as the inhibition of angiogenesis by Albizia Julibrissin.

Inactive Publication Date: 2010-07-07
JIANGNAN UNIV +1
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After investigation, there is no relevant literature report on the anti-angiogenesis effect of Albizia Julibrissin. Therefore, the research fields of anti-angiogenesis pharmacology and pharmacological effects of Albizia Julienne Cortex are still in a blank stage.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application
  • Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application
  • Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment one: the preparation method of the Albizia Julibrissin extract provided by the invention is as follows

[0020] Add 50g of Albizia Julibrissin and 600mL of water to decoct and extract twice, each time for two hours, combine the two water extracts, centrifuge (8000rpm, 20min), take the supernatant, and concentrate it into 100mL of extract. Then, according to the volume ratio of 1:1, the concentrated extract was extracted at room temperature with ether, ethyl acetate, and n-butanol in sequence. Each solvent was extracted 4 times, and the three extraction phases were combined, concentrated and recovered. The organic solution was freeze-dried and stored for later use.

[0021] The experimental results show that the n-butanol extract powder of the water extract of Albizia Julibrissin is white, and the dry matter extraction rate is 0.52%.

Embodiment 2

[0022] Example 2: Inhibitory effect of Albizia Julibrissin extract on proliferation of human vascular endothelial cells.

[0023] Human microvascular endothelial cells (HMEC-1, donated by the French National Institute of Health and Medical Research) were placed in a medium containing 1 mmol glutamine, 1 μg / mL hydrocortisone, 10 ng / mL epidermal growth factor (EGF) and 15% calf serum. Culture in MCDB-131 medium (37°C, 5% CO2, 95% humidity). Take the cells in the logarithmic growth phase, and take 10 5 cell / mL cells were seeded in a 96-well plate, 100 μL / well, with 4 parallel wells for each group, and placed at 37°C, 5% humidified CO 2 incubator overnight. Add different concentrations of diethyl ether, ethyl acetate, n-butanol extracts prepared in Example 1 of the present invention and the residue left after extraction. After cultivating for 72 hours, remove the supernatant, and gently add 10% Trisanol to each well. Fix with 100 μL of chloroacetic acid, let stand for 5 minutes...

Embodiment 3

[0027] Example 3: Albizia Julibrissin Extract Inhibits Angiogenesis of Chicken Embryo Allantoic Membrane (CAM Experiment).

[0028] The experimental sample is the n-butanol extraction part of the water extract of Albizia Julibrissin prepared in Example 1 of the present invention.

[0029] White-skinned eggs, refer to the method of Zhang Shucheng [Zhang Shucheng, Wu Zhikui, Wang Lei, et al. Application of chicken embryo chorioallantoic membrane test model to study the angiogenesis activity and effect of traditional Chinese medicine. Chinese Journal of Basic Medicine of Traditional Chinese Medicine, 1999, 5: 16-19. 】Preparation of chicken embryo allantoic membrane. Microporous membrane discs with a diameter of 6 mm were prepared with a hole punch, and sterilized with moist heat and high pressure. The Albizia Julibrissin extract administration group (250 μg / mL) and the normal saline negative control group were set up. Drop the drug on the filter paper to make a film, and dry it...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of silktree albizia bark extract for inhibiting angiogenesis and practical application; the preparation method is characterized in that: dry bark, stalk, leaves, flower, seed, legume or root of the silktree albizia bark is extracted by water, and then extract fluid is extracted by diethyl, ethyl acetate and n-butyl alcohol sequentially, after separation is carried out, the n-butyl alcohol extract phases are combined and are concentrated to remove the n-butyl alcohol solvent, and then freezing and drying are carried out to obtain the silktree albizia bark extract powder. The silktree albizia bark extract has the application of tumor angiogenesis resistance and treating and controlling the diseases relevant to the angiogenesis, can be used in medicines for inhibiting angiogenesis or can be applied to health-care food for inhibiting angiogenesis or can be applied to cosmetics for inhibiting angiogenesis.

Description

technical field [0001] The invention relates to an extract of Albizia Julibrissin for inhibiting angiogenesis and a preparation method thereof, and clarifies its use in medicine, food and cosmetics, and belongs to the technical field of traditional Chinese medicine application. Background technique [0002] Statistics show that the number of cancer patients in the world is about 10 million every year, and about 7 million people die. It has become the second killer of human beings after cardiovascular disease. There are more than 1.6 million new cancer patients in my country every year, and the number of deaths due to malignant tumors accounts for 17.9% of the total death toll in the country. How to find an anti-tumor drug development direction that suits China's national conditions is a realistic and serious problem facing domestic drug research and development institutions. [0003] At present, drugs for clinical treatment of tumors are mainly divided into two categories. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K8/97A61P3/10A61P9/10A61P15/12A61P17/06A61P17/10A61P19/02A61P27/02A61P27/06A61P29/00A61P35/00A61P35/04A61Q19/00A23L1/30A61K129/00
Inventor 金坚周泉生冯磊邱丽颖花慧陈蕴张莲芬
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com