Molecular marking method for pig litter size

A molecular marker, litter size technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effects of simple operation, easy amplification, and improved accuracy
CN101768642AInactive Publication Date: 2010-07-07INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
Publication Date
2010-07-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a molecular marking method for pig litter size. The method comprises the steps of pig genome DNA extraction, exon 6 primer design of sex hormone binding globulin (SHBG) genes, amplification in vitro and genotype detection. The present invention first discovers pig SHBG genes, discovers the obvious relationship between the polymorphism of exon 6 and pig litter size, and develops a restriction fragment enzyme digestion polymorphism detection method for detecting exon 6 mutational sites. The method can be used for the auxiliary selection of litter size during pig breeding, can realize the early selection of parent pigs and even can accurately select parent pigs when the pigs are born. Generation interval is largely shortened, and selection progress is accelerated. The method has the advantages of simple operation, low condition requirements in polymerase chain reaction processes, short amplification fragment length (217bp), easy amplification, high amplification efficiency and accurate genotype judgement.
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Description

technical field

[0001] The invention belongs to the technical field of animal gene polymorphism, in particular to the polymorphism of sex hormone binding globulin (SHBG) gene of pigs. Background technique

[0002] Sex hormone-binding globulin (Sex Hormone-Binding Globulin, SHBG), also known as testosterone-estradiol-binding globulin, SHBG is a carrier for transporting sex hormones. The arrival of animal sex hormones to target tissues is regulated by SHBG in the blood. SHBG is A glycoprotein expressed in liver cells, this protein specifically binds to and participates in the transport of sex hormones, and regulates the concentration of biologically active sex hormones in the blood. Under physiological conditions, a dynamic balance exists between the rapid dissociation of the sex hormone-binding globulin (SHBG) complex and the association of free sex hormones with SHBG, which are protected from vascular adsorption, biological and chemical Destruction and rapid degradation, on...

Claims

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