Molecular marking method for pig litter size

A molecular marker, litter size technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effects of simple operation, easy amplification, and improved accuracy

Inactive Publication Date: 2010-07-07
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0004] At present, since there is no research report on the pig SHBG gene, the Anhui Academy of Agricultural Sciences set up a project to study the structure and polymorphism analysis of the pig SHBG gene, and successfully isolated the sequence of the fourth exon to the sixth exon of the pig SHBG gene , established a detection method for exon 6 mutants, and analyzed the correlation between its polymorphisms and litter size

Method used

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  • Molecular marking method for pig litter size
  • Molecular marking method for pig litter size
  • Molecular marking method for pig litter size

Examples

Experimental program
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Embodiment

[0053] A specific operation method of a molecular marker method for pig litter size traits is as follows:

[0054] (1) Collection of ear tissue samples

[0055] On the day of the piglet's birth, about 0.2 grams of the pig's ear margin tissue was collected with pig ear forceps, put into a 1.5ml centrifuge tube, poured about 1ml of 75% alcohol, and then stored in a -20°C refrigerator for later use.

[0056] (2) Genomic DNA extraction

[0057] Take out the ear margin tissue from the refrigerator, cut it into pieces with scissors, and pour 600ul of DNA tissue extraction solution. The formula of the DNA tissue extraction solution is shown in Table 1, and the extraction solution is ready for use. Then put it in a warm bath at 55°C for 12 hours, then add 600ul of Tris saturated phenol, shake it up and down for 15 minutes, and centrifuge for 10 minutes in a low-temperature high-speed centrifuge with a speed of 12000 rpm; take out the supernatant with a 1ml pipette To a sterilized 1....

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Abstract

The present invention relates to a molecular marking method for pig litter size. The method comprises the steps of pig genome DNA extraction, exon 6 primer design of sex hormone binding globulin (SHBG) genes, amplification in vitro and genotype detection. The present invention first discovers pig SHBG genes, discovers the obvious relationship between the polymorphism of exon 6 and pig litter size, and develops a restriction fragment enzyme digestion polymorphism detection method for detecting exon 6 mutational sites. The method can be used for the auxiliary selection of litter size during pig breeding, can realize the early selection of parent pigs and even can accurately select parent pigs when the pigs are born. Generation interval is largely shortened, and selection progress is accelerated. The method has the advantages of simple operation, low condition requirements in polymerase chain reaction processes, short amplification fragment length (217bp), easy amplification, high amplification efficiency and accurate genotype judgement.

Description

technical field [0001] The invention belongs to the technical field of animal gene polymorphism, in particular to the polymorphism of sex hormone binding globulin (SHBG) gene of pigs. Background technique [0002] Sex hormone-binding globulin (Sex Hormone-Binding Globulin, SHBG), also known as testosterone-estradiol-binding globulin, SHBG is a carrier for transporting sex hormones. The arrival of animal sex hormones to target tissues is regulated by SHBG in the blood. SHBG is A glycoprotein expressed in liver cells, this protein specifically binds to and participates in the transport of sex hormones, and regulates the concentration of biologically active sex hormones in the blood. Under physiological conditions, a dynamic balance exists between the rapid dissociation of the sex hormone-binding globulin (SHBG) complex and the association of free sex hormones with SHBG, which are protected from vascular adsorption, biological and chemical Destruction and rapid degradation, on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李庆岗陶立刘林清
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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