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A technology of molecular markers and growth traits, applied in the field of molecular markers of pig growth traits, can solve problems such as unclear mechanisms, and achieve the effect of shortening the generation interval
Active Publication Date: 2022-02-25
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Expression of calbindin 3, like that of calbindin 1, is activated by the active vitamin D metabolite calcitriol, but the exact mechanism remains unclear
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Embodiment 1
[0048] The ear margin tissues of 390 large Yorkshire pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:
[0049] (1) Extracting the genomic DNA of the Great Yorkshire pig
[0050] Extraction of porcine genomic DNA from ear margin tissue of Large Yorkshire pigs;
[0051] (2) Primer design
[0052] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:
[0079]The ear margin tissues of 235 Duroc pigs were collected, and their growth rate was measured at the same time, that is, the age at 100 kg body weight. Take it back to the laboratory, and carry out the genotype detection of the molecular marker CALB2 gene of pig growth traits respectively. The specific operation steps are as follows:
[0080] (1) Genomic DNA extraction of Duroc pigs
[0081] Extraction of porcine genomic DNA from ear margin tissues of Duroc pigs;
[0082] (2) Primer design
[0083] A pair of primers were designed between -1201bp and -654bp of the start transcription site of the pig calcium binding protein 2 (CALB2) gene, that is, the upstream primer and the downstream primer. The sequences of the upstream primer and the downstream primer are as follows:
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Abstract
The invention relates to a molecular marker method for growth traits of pigs. The operation steps are as follows: extract porcine genomic DNA from porcine ear margin tissue; design a pair of primers between -1201bp and -654bp of the initiation transcription site of porcine calcium-binding protein 2 (CALB2) gene, that is, upstream primers and downstream primers Obtain the 548bp long in vitro amplification product between the upstream -1201bp and -654bp of the pig CALB2 gene transcription start site by polymerase chain reaction; Carry out the restriction endonuclease HhaI enzyme digestion reaction to the in vitro amplification product; The cut product was tested for polymorphism (RLFP), and the AA type was obtained as the genotype with excellent growth speed, that is, the mutant type. Therefore, the piglets with the AA type were selected for breeding, and the growth and fattening cycle of the AA type pigs was shorter than that of the GG type. Pigs shortened by 3.6 to 4.0 days.
Description
technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a molecular marker method for growth traits of pigs. Background technique [0002] Growth rate is an important indicator that affects the economic benefits of pig producers. The shorter the feeding cycle of pigs with faster growth rate, the lower the cost required, and the higher income pig producers can obtain. The traditional breeding of pig growth speed is to measure the weight of pigs one by one when they grow to 85kg to 130kg. At this time, the age is about 150-180 days. Then select and retain, time-consuming, labor-intensive, and capital-consuming. [0003] Calbindin refers to the general term for several calcium-dependent binding proteins. It was originally a vitamin D-dependent calcium-binding protein found in the kidney and small intestine of chickens and mammals. Because of the hand-shaped domain of calcium-binding protein ( EF-hand) is divided into d...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 李庆岗吴义景苏世广杨家军
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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