Interface unit containing capillary electrophoresis of hollow-fiber membrane and chemoluminescence combination

Abstract

The invention relates to an interface unit containing capillary electrophoresis of a hollow-fiber membrane and chemoluminescence combination, which at least comprises a hollow-fiber membrane. The hollow-fiber membrane is arranged between an injection port of a capillary and a detection window, both ends of the hollow-fiber membrane are respectively inserted into a Teflon tube, and the edges for connecting the Teflon tube with the hollow-fiber membrane are bonded and sealed by epoxide resin; chemiluminescence reagent is mixed with a sample through the hollow-fiber membrane, the chemiluminescence reaction is carried out, and a chemiluminescence detector is used for further detection. The interface unit has the advantages of high column efficiency and detection sensitivity, simple structure,convenient operation, easy control and wide application range.

Description

【Technical field】 [0001] The invention relates to the technical field of capillary electrophoresis, in particular to an interface device for combining capillary electrophoresis and chemiluminescence with a hollow fiber membrane. 【Background technique】 [0002] Capillary electrophoresis (CE), a new separation technique developed in the 1980s, has become one of the most active research directions in analytical chemistry. While capillary electrophoresis has the advantages of high efficiency, rapidity, less sample volume and less reagent consumption, it is also restricted by the difficulty of micro sample detection. Solving the problem of capillary electrophoresis micro sample detection is one of the key technologies for the further development of this method . Combined with the highly sensitive and convenient chemiluminescence detection technology, the application range of capillary electrophoresis can be broadened to a certain extent. The combination of capillary electrophor...

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Problems solved by technology

For the sleeve-type interface, experiments have proved that this interface technology has many advantages, but there are also some obvious deficiencies: ①Because there are gaps in the interface, it is easy to introduce air bubbles to cut off the capillary electrophoresis, and the detection cannot be carried out; ②When the analyte is separated from the electrophoresis When the tube flows out into a reaction tube with a larger inner diameter and mixed with the luminescent reagent, it is easy to cause eddy current diffusion, resulting in peak broadening and lower column efficiency; ③The mixing of luminescent reagent and analyte is not uniform and stable, which affects the reproducibility; ④Luminescent reagent The flow rate needs to match the flow rate of the analyte in the separation tube. If the flow rate of the luminescent reagent is too high, the detected optical signal will be incomplete, which will affect the peak height or peak area. Otherwise, it will be caused by staying in the detection window for too long. Peak broadening; these problems limit the range of applications of chemiluminescence as a capillary electrophoresis detector

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