System for on-line desalinization, enrichment and mass spectrum of Double tap upgrade liquid chromatogram

A liquid chromatography and desalting technology, which is applied in the field of enrichment and mass spectrometry combined system, double-split nano-upgrade liquid chromatography on-line desalination, can solve the problems of poor separation effect, poor system repeatability, unfavorable automation, etc., and achieves the application scope Wide, good versatility, strong practicability
CN101206197BInactive Publication Date: 2011-04-06徐州脉析医疗技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
徐州脉析医疗技术有限公司
Publication Date
2011-04-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a double splitflow nano upgrade liquid phase chromatogram online desalting, enrichment and mass spectrum combination system which comprises a pump, a first splitter, a second splitter, a sample valve, a switching valve, a backpressure valve, a capillary tube separation column and a mass spectrometer, wherein, the pump, the first splitter, the sample valve, the second splitter, the capillary tube separation column and the mass spectrometer are connected in series in turn through pipelines; the split ports of the first splitter and the second splitter are respectively connected with the two inlets of the switching valve through the pipelines; moreover, the outlet of the switching valve is connected in series with the backpressure valve and then is communicated with a waste fluid pool through the pipeline. The system has the advantages that: during the sampling and separation of sample, the system has less pressure fluctuation; with small pre-column dead volume, the system overcomes the gradient delay effect caused by large-volume sampling adopted in the prior nano upgrade liquid phase separation system; with excellent separation repeatability, the system is particularly suitable for gradient separation; meanwhile, the system can be used in online desalting, enrichment and mass spectrum combination of sample with high salinity and low concentration; moreover, the system has a simple structure, convenient operation and easily realized automation.
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Description

technical field

[0001] The invention relates to a micro-separation system, in particular to a dual-split flow nano-upgrade liquid chromatography on-line desalination, enrichment and mass spectrometry system. Background technique

[0002] Since the 1980s, with the development of chromatographic theory, people began to realize the advantages of micro-separation systems, and devoted themselves to the research and development of micro-scale, micro-flow high performance liquid chromatography (HPLC) separation systems. In such systems, fused silica capillary tubes, PEEK tubes or PEKSIL tubes with an inner diameter of 20-250 μm are generally used as column tubes, and filled with fillers. Separation of samples at a low flow rate of 0.02-10 μL / min can obtain higher column efficiency and resolution than conventional HPLC systems. At the same time, in such a micro-scale system, the traditional UV detector is no longer suitable due to its short detection optical path and low detection ...

Claims

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