Heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof

A technology of capillary electrophoresis and heart cutting, which is applied in the direction of measuring devices, scientific instruments, and material analysis through electromagnetic means, to achieve high sensitivity, eliminate dead volume, and avoid loss and dilution effects

Inactive Publication Date: 2010-10-06
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there have been no reports on the combination of two-dimensional capillary electrophoresis with on-column electrochemical detection and the use of online enrichment technology for two-dimensional capillary electrophoresis to improve column efficiency and detection sensitivity.

Method used

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  • Heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof
  • Heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof
  • Heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof

Examples

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Effect test

Embodiment 1

[0023] Example 1 Construction of heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device

[0024] A specific heart-cutting 2D capillary electrophoresis on-column electrochemical setup such as figure 1 shown. At 1.0cm away from the outlet end of capillary 2 (50μm.d., 375μm o.d., Hebei Yongnian Ruifeng Chromatographic Devices Co., Ltd.), remove a section of polyimide coating on one side of the capillary, and place the part of the capillary that has been removed in a hydrofluoric It is etched into a porous shape in the acid solution, and a layer of cellulose acetate pulp is evenly coated on the place. After solidification, a field isolation joint that only passes current and no solution leakage is formed; the field isolation joint is placed in polytetrafluoroethylene In the cell body 4, there are capillary tube access openings and electrode sockets in the cell body 4; the capillary tube 2 and the above-mentioned treated field isolation joint are ...

Embodiment 2

[0025] Example 2 Application of heart-cutting two-dimensional capillary electrophoresis to separation and detection of 9 drug compounds in blood samples with a column electrochemical device. Experimental conditions:

[0026] The nine drug compounds are: propranolol, acebutolol, metoprolol, propranolol, labetalol, timolol, pindolol, atenolol, and nadolol Er (USA, Sigma Company), sodium lauryl sulfate, glacial acetic acid, anhydrous sodium acetate, and acetonitrile were all analytically pure (Shanghai Chemical Reagent Company), other reagents were analytically pure, and the experimental water was double distilled water.

[0027] The first dimension separation adopts capillary zone electrophoresis, the separation buffer is 90mmol / L sodium acetate, and the pH is adjusted to 4.3 with concentrated acetic acid; the second dimension separation adopts micellar electrokinetic capillary chromatography, and the separation buffer is 15mmol / L sodium acetate+30mmol / L sodium lauryl sulfate, ...

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Abstract

The invention relates to a capillary tube electrophoretic technique, in particular to a heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof. The device comprises a capillary column, an electrochemical detection pool, an electrochemical detector and a high-voltage power supply, wherein a segment of polyimide coating is removed from a position with a distance of 0.5 to 3 cm away from the outlet end of the capillary; the capillary wall with the coating removed is corroded into a porous shape by using hydrofluoric acid; a layer of cellulose acetate pulp is coated on the position uniformly to form a field insulating joint which can only allow current to pass through and avoid leakage of solution; and a working electrode, an auxiliary electrode and a reference electrode are inserted into the electrochemical detection pool. The application comprises the following steps of: separating a complicated sample in first dimension buffer solution; when the working electrode detects a signal of a target component, applying pressure at a detection end to drive the target component to the inlet end of the capillary; allowing second dimension separation buffer solution to enter the capillary from the tail end of the capillary; and further performing separation and detection on the target component in the second dimension separation buffer solution. The device has the advantages of high column efficiency of heart-cutting two-dimensional capillary electrophoresis separation, no loss of samples and suitability for on-line separation and detection of target components in complicated samples.

Description

technical field [0001] The invention relates to capillary electrophoresis technology, in particular to a heart-cut two-dimensional capillary electrophoresis on-column electrochemical device and its application. Background technique [0002] Capillary electrophoresis is widely used in chemical, biological, clinical and other fields due to its advantages of high separation efficiency and fast analysis speed. When analyzing complex samples by capillary electrophoresis, in order to analyze the target components in the complex samples and eliminate the interference of the sample matrix, sample pretreatment is usually performed. Commonly used sample pretreatment methods include liquid phase extraction, solid phase extraction, and solid phase microextraction. These sample pretreatment methods are cumbersome, time-consuming and labor-intensive. Due to its large peak capacity, high resolution and strong separation ability, two-dimensional capillary electrophoresis has attracted muc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 张召香杜秀珍
Owner QINGDAO UNIV OF SCI & TECH
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