Fish soaking vaccine combination adjuvant and application and using method thereof

A technology for soaking vaccines and adjuvants, which is applied in the field of combined adjuvants for soaking vaccines for fish, can solve problems such as low work efficiency, fish body damage, and few products, and achieves the effects of simple and convenient use, improved immune effect, and safe use.

Inactive Publication Date: 2010-08-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fish injection vaccines have obvious effect on disease prevention, but injection immunization workload is heavy, work efficiency is low, and it will cause damage to fish
The fish soaking vaccine does not have the above disadvantages, but the simple use of vaccine soaking effect is not good
Currently, there are not many vaccines developed for fish immersion immunization due to the limitation of poor immersion immunization effect.
Domestically developed adjuvants for fish soaked vaccines include hyoscyamine, dextran, and IMS1312. Tests have shown that the immune protection rate after use is not ideal
Most foreign fish-soaked immune adjuvants are secret formulas
Based on the above status quo, the speed of research and development of fish soaked vaccines in my country is in a state of slow development, and restrictions hinder the development of the industry

Method used

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  • Fish soaking vaccine combination adjuvant and application and using method thereof
  • Fish soaking vaccine combination adjuvant and application and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: application concentration of the present invention is respectively 1mg / L, 0.1mg / L, 60mg / L DTT, VD3, 654-2 combined adjuvant and inactivated Vibrio anguillarum (Vibrio.Anguillarum) vaccine to flounder immersion immunity

[0017] 1 Material:

[0018] (1) Test fish and breeding management: select healthy flounder with a weight of 5g, and raise them temporarily for 1 week before the test. The test seawater is stable at 20-23°C, pH8.1-8.2, salinity 30, and fed with compound bait during the test. , fed twice a day, continuously inflated for 24 hours, running water culture.

[0019] (2) Vaccine preparation: inoculate V. anguillarum into 2216E liquid medium, incubate at 28°C for 24h; centrifuge at 4000rpm at 4°C for 20min to collect the bacteria; place the collected bacteria in sterilized PBS, pH 7.2, to prepare Vibrio suspension of 1.0×108cells / mL, inactivated by 0.5% formaldehyde at 28°C for 48 hours, then stored at 4°C; at the same time, the inactivated bacte...

Embodiment 2

[0030] Embodiment 2: Application concentration of the present invention is respectively 50mg / L, 20mg / L, 1mg / L combined adjuvant of DTT, VD3, 654-2 and inactivated Aeromonas dot-shaped vaccine to tilapia immersion immunization

[0031] 1 Material:

[0032] (1) Test fish and breeding management: choose healthy tilapia with a weight of 3g, and temporarily raise them for 10 days before the test. Feed 2 times, change 30% of the water daily, and inflate continuously for 24 hours.

[0033] (2) Vaccine preparation: Inoculate Aeromonas punctatus into LB liquid medium, culture at 28°C for 24h; centrifuge at 4000rpm for 20min at 4°C, collect the bacteria; place the collected bacteria in sterilized normal saline, In pH 7.2, make a bacterial suspension of 1.0×109cells / mL, inactivate 0.5% formaldehyde at 28°C for 48 hours, then store it at 4°C; Cultivate at a constant temperature of 28°C for 24h and 48h, and confirm that no colony grows.

[0034] (3) Immune adjuvants soaked: DTT, VD3, 65...

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PUM

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Abstract

The invention relates to a fish soaking vaccine combination adjuvant and application and a using method thereof. The invention is characterized in that the combination adjuvant is the combination of three adjuvants which are respectively dithiothreitol, Vitamin D3 and racanisodamine, wherein the using concentrations of dithiothreitol, Vitamin D3 and racanisodamine are respectively 1-50mg / L, 0.1-20mg / L and 1-60mg / L; the fish soaking vaccine combination adjuvant is applied to the soaking vaccine comprising seawater and freshwater fishes, and the synergistic effect reaches the efficient immunity effect. The using method is as follows: firstly, selecting the fish with more than 2g weight and sound body surface to be immunized, and immigrating into dithiothreitol pretreatment solution with 1-50mg / L of the concentration range to be soaked for 1-20min, then adding VD3 with 1-20mg / L of the concentration range and vaccine to be soaked for 1-20min thereinto, and finally adding racanisodamine with 1-60mg / L of the concentration range to be soaked for 5-20 min. The fish soaking vaccine combination adjuvant obviously increases the immune effect of the vaccine; the using method is simple, convenient and safe, has low cost, does need additional facilities and working procedures, does not affect the cultivating livability and the growth speed of the fishes, and is simultaneously harmless to the environment and operating personnel.

Description

technical field [0001] The invention relates to the immune prevention and control technology of marine biological diseases, in particular to a combination adjuvant for fish soaked vaccine and its application and use method. Background technique [0002] The output of farmed fish in my country ranks first in the world, but the research and development of fish disease prevention and control technology is slow, and the developed fish disease prevention and control products cannot meet the needs of production, especially in the development and application of fish vaccines, which are currently available for fishery production. Not many products to choose to use. Therefore, the treatment of fish bacterial diseases in aquaculture relies too much on feeding antibiotics. As a result, long-term application of antibiotics will not only reduce the quality of aquatic products, but also lead to enhanced bacterial resistance, making it more difficult for future disease control. Foreign cou...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 王秀华黄倢王玉娟赵伟伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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