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Method for separating Chinese white poplar ovule from nucellus

A technique of poplar tomentosa and ovule, applied in plant cells and other directions, can solve the problems of difficulty in maintaining the original shape of the bead center, and the difficulty of manually separating the ovule and the bead center, so as to achieve the effect of dexterous force application, reducing damage and buffering force.

Inactive Publication Date: 2012-01-25
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The ovules of Populus tomentosa are small, and it is difficult to separate the ovules and nucellus by hand. It is difficult to maintain the original shape of the nucellus separated by non-enzymatic technology.

Method used

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  • Method for separating Chinese white poplar ovule from nucellus
  • Method for separating Chinese white poplar ovule from nucellus
  • Method for separating Chinese white poplar ovule from nucellus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method that present embodiment separates Populus tomentosa ovule comprises the following steps:

[0026] Preparation of rubber blocks: Take 2 pieces of chewing gum, rub them in warm water, soak them several times, to fully wash away the sugar and other substances in the chewing gum, knead the washed rubber blocks into balls, and stick them in the center of the field of view of the dissecting mirror for later use ;

[0027] Separation of ovules of Populus tomentosa: Pick the female flowers of Populus tomentosa from the female inflorescence, stick them on the glue block, remove the bracts and oblique cup-shaped flower discs of the female flowers under a dissecting microscope, and cut the ovules along a dorsal suture. The chamber wall was cut longitudinally, then opened and its outer wall was glued on the glue block. It can be seen that there are two ovules in the ovary chamber (such as figure 2 Shown), the funicicle under the ovule is long and strong, just separate ...

Embodiment 2

[0029] The method that present embodiment separates Populus tomentosa ovule comprises the following steps:

[0030] Preparation of gum blocks: Take 3 pieces of chewing gum, put them into warm water, knead and soak them several times to fully wash away the sugar and other substances in the gum, knead the washed gum blocks into balls, and stick them in the center of the field of view of the dissecting mirror for later use ;

[0031] Separation of ovules of Populus tomentosa: Pick the female flowers of Populus tomentosa from the female inflorescence, stick them on the glue block, remove the bracts and oblique cup-shaped flower discs of the female flowers under a dissecting microscope, and cut them along the two dorsal sutures. The ovary was cut longitudinally, and the two ovary walls were peeled off and glued to the glue block, and it was observed that the funicular stalk under each ovule was born at the base of the two carpels (such as image 3 Shown), one side of each funiculu...

Embodiment 3

[0033] The method that present embodiment separates poplar tomentosa nucellus comprises the following steps:

[0034] Populus tomentosa ovules were isolated by conventional separation methods;

[0035] The isolated ovules were stained with dilute hematoxylin, and transparentized in a mixture of absolute ethanol and xylene at a volume ratio of 1:1, and observed on concave glass slides to understand the ovules. structural features (such as Figure 6 shown);

[0036] Then stick the newly separated ovules and pedicel on the glue block, and after fixing, use a dissecting needle to gently cut the integument of the ovule upwards at the junction of the ovule and the pedicel, and then follow the same procedure around it. The method is to select one or several places and cut the integument of the ovule gently from bottom to top, use a dissecting needle to gently lift the integument between the two cuts from bottom to top, and stick the outer wall of the lifted integument on the On th...

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Abstract

The invention discloses a method for separating Chinese white poplar ovule from nucellus, wherein female flower of the Chinese white poplar is picked and put on a gel block, bract and floral disc of the female flower are removed with the help of an anatomical lens, the ovary of the female flower is longitudinally cut open along a dorsal suture on the sidewall of the ovary, so funicle and ovule can be separated off from carpel; when the nucellus is separated, the separated funicle and the ovule can still be put on the gel block, integument of the ovule is cut open longitudinally and upwards, picked and lifted from bottom to top from the juncture of the ovule and the funicle, the step of cutting, picking and lifting the integument is repeated so that the nucellus can be exposed gradually, and in the end, the nucellus is cut off at the junction of the ovule and the integument so as to be completely separated from the integument. The method can not only avoid the influence of enzymatic method on the cultivation and growth performance of plant materials, but can also reduce the damage brought by non-enzymatic method and outstandingly maintain the original shape of materials to be separated.

Description

technical field [0001] The invention relates to a method for separating ovules of Populus tomentosa and a method for separating nucellus of Populus tomentosa, belonging to the technical field of plant microscopic observation. Background technique [0002] The ovules of Populus tomentosa are tiny, and it is difficult to separate the ovules and nucellus by hand, and the nucellus separated by non-enzymatic technology is difficult to maintain its original shape. At present, mostly by means of enzymatic hydrolysis technologies such as cellulase and pectinase, the artificial separation of microstructures such as plant nucellus, embryo sac, egg cell and zygote is completed (the paper "Angiosperm Embryo Sac" published by Zhou Chang et al. in 1982) Research on Enzymatic Separation: Separation Technology and Microscopic Observation of Fixed Materials", Yang Hongyuan et al. published a paper in 1998 "Research Progress in In Vitro Fertilization and Zygote Culture of Angiosperms"). Beca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 洪亚平张亚冰
Owner HENAN UNIV OF SCI & TECH
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