Culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium

The invention relates to a technology for the adsorption capacity of Ruchella yeast, which is applied in the cultivation field of improving the adsorption capacity of the heavy metal cadmium of Russellii, and can solve the problems such as the pollution of the treated liquid.

Inactive Publication Date: 2010-10-06
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to improve the abilit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1. Add 10g of yeast extract, 18g of peptone, 20g of glucose and 5% sodium chloride to 1000mL of distilled water; slowly heat up until it is completely dissolved to obtain a liquid medium, then use 0.1mol / L of hydrochloric acid and hydrogen Adjust the pH of the medium with sodium oxide solution to 6.0, and sterilize at 115°C for 20 minutes.

[0019] Step two: inoculate the liquid seeds of the activated yeast Lu's yeast into the medium obtained in step one. The culture conditions of the controlled shake flask were: constant temperature 28°C, shaker rotation speed 180rpm, liquid volume 100ml / 500ml, inoculation volume 4%, culture for 24h.

[0020] In step three, the fermentation broth obtained in step two was centrifuged at 4000 rpm for 20 minutes to collect the bacteria, the surface medium was washed, and then freeze-dried to prepare a powder.

Embodiment 2

[0022] Step 1. Add 10g of yeast extract, 22g of peptone, 20g of glucose and 10% potassium chloride into 1000mL of distilled water successively; slowly warm up until it is completely dissolved to obtain liquid culture medium, and then use 0.1mol / L hydrochloric acid and hydrogen Adjust the pH of the medium with sodium oxide solution to 6.2, and sterilize at 115°C for 20 minutes.

[0023] Step two: inoculate the liquid seeds of the activated yeast Lu's yeast into the medium obtained in step one. The culture conditions of the fermenter were: constant temperature 28°C, rotation speed 300 rpm, liquid loading coefficient 0.6, inoculation amount 10%, ventilation rate 1 vvm, and culture for 48 hours.

[0024] In step three, the fermentation broth obtained in step two is centrifuged at 5000 rpm for 10 minutes to collect the bacteria, the surface medium is washed, and freeze-dried to prepare a powder.

Embodiment 3

[0026] Step 1. Add 10g of yeast extract, 20g of peptone and 200g of glucose to 1000mL of distilled water successively; slowly increase the temperature until it is completely dissolved to obtain a liquid medium, then adjust the medium with 0.1mol / L hydrochloric acid and sodium hydroxide solution pH to 6.0, sterilize at 115℃ for 20min.

[0027] Step two: inoculate the liquid seeds of the activated yeast Lu's yeast into the medium obtained in step one. The culture conditions of the controlled shake flask were: constant temperature of 30°C, shaker speed of 160rpm, liquid volume of 100ml / 500ml, inoculation volume of 4%, and culture for 42 hours.

[0028] In step three, the fermentation broth obtained in step two was centrifuged at 6000 rpm for 10 minutes to collect the bacteria, the surface medium was washed, and freeze-dried to prepare a powder.

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Abstract

The invention discloses a culture method for improving adsorption capacity of zygosaccharomyces rouxii heavy metal cadmium, which belongs to the technical field of food biology. The culture method for improving the adsorption capacity of the zygosaccharomyces rouxii heavy metal cadmium comprises the following steps of: preparing a culture medium containing 5 to 12 grams of yeast powder, 15 to 25 grams of peptone, 15 to 200 grams of glucose, 1,000mL of distilled water and 0.01 to 20 percent of sodium chloride or potassium chloride; adjusting the pH of the culture medium to be between 5.8 and 6.4, and sterilizing the culture medium for 20 minutes at the temperature of 115 DEG C; and inoculating zygosaccharomyces rouxii to the culture medium, culturing the zygosaccharomyces rouxii by liquid in a shaking bed or a fermentation tank at the temperature of between 28 and 30 DEG C to obtain zygosaccharomyces rouxii cells, and preparing freeze-drying powder. The adsorption capacity of the zygosaccharomyces rouxii dry cadmium powder is improved by 0.5 to 15 percent by adopting the method of the invention. The method of the invention is simple, good in safety and easy to implement, and has significance for clearing cadmium ions in water body, protein enzymolysis liquid, Chinese medicinal herb decoction and the like.

Description

Technical field [0001] The invention relates to a culture method for improving the heavy metal cadmium adsorption capacity of Lu's yeast, and belongs to the field of food biotechnology. Background technique [0002] Environmental pollution leads to pollution of waters, causing the content of cadmium in some food raw materials to exceed the standard. Cadmium accumulates along the food chain in organisms, threatening human health. Microbial adsorption is a very effective method to solve the problem of heavy metal pollution, and its application is becoming more and more common. [0003] Yeast Rousei is a commonly used flavour-enhancing bacteria for fermented soy sauces and sauces. There is currently no literature that proves that Yeast Rousei is pathogenic to humans. Lu's yeast is easy to cultivate, grows fast, has strong salt tolerance, can still grow in a solution containing 24% salt, and has the ability to absorb heavy metal cadmium in water. The invention adopts a special cultur...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F1/28C02F101/20C12R1/645
Inventor 徐莹汪东风李春生刘婍
Owner OCEAN UNIV OF CHINA
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