Method for preparing three-dimensional cell chip
A three-dimensional cell and chip technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low reliability and accuracy of research results, and achieve high reliability and accuracy
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Embodiment 1
[0020] Example 1: Preparation of three-dimensional mouse bladder smooth muscle cell chip
[0021] A method for preparing a three-dimensional cell chip, comprising the following specific steps:
[0022] The first step is to prepare a sol solution for forming a micropore array, mix a hydrogel macromer, a crosslinking agent and a hydrogel solvent in a mass ratio of 1:0.1:100 to form the first sol solution, and the hydrogel It is polyethylene glycol, the cross-linking agent is 2-hydroxy-methylphenylpropan-1-one, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 7.5.
[0023] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer and the hydrogel solvent in a mass ratio of 1:200 to form the second sol solution, the hydrogel is collagen, and the hydrogel solvent for pure water,
[0024] The third step is to prepare the microwell array. Take 1ml of the first sol solution and place it on a methylate...
Embodiment 2
[0028] Example 2: Preparation of a three-dimensional sheep bone marrow pluripotent stromal cell chip
[0029] A method for preparing a three-dimensional cell chip, comprising the following specific steps:
[0030] The first step is to prepare a sol solution for forming a micropore array, mix the hydrogel monomer and the hydrogel solvent at a mass ratio of 1:200 to form the first sol solution, the hydrogel is collagen, and the hydrogel solvent is pure water,
[0031] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer, the cross-linking agent and the hydrogel solvent in a mass ratio of 1:0.05:100 to form the second sol solution, and the hydrogel is Hyaluronic acid, the cross-linking agent is dimethacrylic anhydride, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH value of 8.0,
[0032] The third step is to prepare the microwell array, take 2ml of the first sol solution and place it on a methylate...
Embodiment 3
[0036] Example 3: Preparation of a three-dimensional human neural stem cell chip
[0037] A method for preparing a three-dimensional cell chip, comprising the following specific steps:
[0038] The first step is to prepare a sol solution for forming a micropore array, mix a hydrogel macromer, a crosslinking agent and a hydrogel solvent in a mass ratio of 1:0.5:50 to form the first sol solution, and the hydrogel Chitosan, the cross-linking agent is β-sodium glycerophosphate, the hydrogel solvent is deionized water,
[0039] The second step is to prepare the sol solution mixed with the cell culture medium, mix the hydrogel macromer, the cross-linking agent and the hydrogel solvent in a mass ratio of 1:0.01:10 to form the second sol solution, and the hydrogel is Polyethylene glycol, cross-linking agent is 2-hydroxyl-methylphenyl propane-1-ketone, and hydrogel solvent is the phosphate (PBS) buffer solution that pH value is 5.7,
[0040] The third step is to prepare the microwell...
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