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Primer group and kit for detecting human platelet alloantigen gene

An alloantigen and primer set technology, applied in recombinant DNA technology, DNA/RNA fragments, etc.

Active Publication Date: 2012-04-18
天津市秀鹏生物技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no application of this method to realize the rapid detection of human platelet alloantigen HPA (1-17) gene

Method used

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  • Primer group and kit for detecting human platelet alloantigen gene
  • Primer group and kit for detecting human platelet alloantigen gene
  • Primer group and kit for detecting human platelet alloantigen gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] According to the HPA allele sequence released by the National Center for Biotechnology Information (NCBI Gene Bank), a primer set for detecting the human platelet alloantigen gene was designed. The primer set included HPA-6a primer pair, HPA-6b Primer pair, HPA-15Gov a Primer pair, HPA-15Gov b Primer pairs and internal reference primer pairs.

Embodiment 2

[0081]According to the HPA allele sequence released by the National Center for Biotechnology Information (NCBI Gene Bank), a primer set for detecting the human platelet alloantigen gene was designed, and the primer set included HPA-1a primer pair, HPA-1b Primer pair, HPA-2a primer pair, HPA-2b primer pair, HPA-3a primer pair, HPA-3b primer pair, HPA-4a primer pair, HPA-4b primer pair, HPA-5a primer pair, HPA-5b primer pair , HPA-6a primer pair, HPA-6b primer pair, HPA-15Gov a Primer pair, HPA-15Gov b Primer pair, HPA-7a primer pair, HPA-7b primer pair, HPA-8a primer pair, HPA-8b primer pair, HPA-9a primer pair, HPA-9b primer pair, HPA-10a primer pair, HPA-10b primer pair , HPA-11a primer pair, HPA-11b primer pair, HPA-12a primer pair, HPA-12b primer pair, HPA-13a primer pair, HPA-13b primer pair, HPA-14a primer pair, HPA-14b primer pair, HPA - 16a primer pair, HPA-16b primer pair, HPA-17a primer pair, HPA-17b primer pair and internal reference primer pair.

Embodiment 3

[0083] A kit for detecting human platelet alloantigen gene, comprising a pair of primers coated with HPA-6a, a pair of primers for HPA-6b, a pair of HPA-15Gov a Primer pair, HPA-15Gov b Primer pairs, primer plates for internal reference primer pairs, and enriched dNTP-Buffer,

[0084] The preparation method of the test kit for detecting human platelet alloantigen gene is:

[0085] (1) According to the HPA (1-17) specific primer hole map (see figure 2 ), HPA-6a primer pair, HPA-6b primer pair, HPA-15Gov a Primer pair, HPA-15Gov b The primer pair and the internal reference primer pair are coated on the corresponding positions of the primer plate and dried;

[0086] (2) According to the formula 200mM dNTP, 3.5mM Mg 2+ , 500mM KCL, 100mM Tris-HCL, 1% TritonX-100, prepared 440μl concentrated dNTP-Buffer;

[0087] (3) The primer plate coated with the primer pair and the concentrated dNTP-Buffer are used to form a kit for detecting human platelet alloantigen gene.

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PUM

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Abstract

The invention discloses a primer group and a kit for detecting a human platelet alloantigen (HPA) gene. The primer group for detecting the human platelet alloantigen (HPA) gene comprises an HPA-6a primer pair, an HPA-6b primer pair, an HPA-15Gova primer pair, an HPA-15Govb primer pair and an internal reference primer pair. The kit of the invention realizes platelet genotyping and has great significance for effectively avoiding platelet specific immunity and selecting to transfuse blood products matched with HPA genotypes in clinical and blood transfusion.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a primer set for detecting human platelet alloantigen gene and a kit for detecting human platelet alloantigen gene. Background technique [0002] There are complex blood group antigens on the surface of human platelets, and the platelet antigens are distributed according to their distribution. Can be divided into two types. One is antigens shared with other cells or tissues, such as ABH, HLA-I antigens, etc.; the other is platelet-specific alloantigen (HPA), which is composed of platelet-specific antigenic determinants. HPA mismatch may Cause platelet alloimmune reaction, produce platelet alloantibody, generally produced by immune stimulation such as blood transfusion, pregnancy or bone marrow transplantation, lead to alloimmune thrombocytopenia, induce such as post transfusion thrombocytopenic purpura (PTP), platelet transfusion Ineffective (PTR), passive thrombocytopenia (PAIT) and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 赵卫军
Owner 天津市秀鹏生物技术开发有限公司
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