A method and application for quickly establishing the slow feather line of Beijing oil chicken

An oily and rapid technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of high cost, long time-consuming test cross test, etc. The effect of simple experimental design

Active Publication Date: 2019-06-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a fluorescent quantitative PCR method for quickly identifying the feather speed genotype of Beijing oil chicken roosters in order to solve the problems of the shortcomings of long time-consuming and high cost in the test cross test in the prior art

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  • A method and application for quickly establishing the slow feather line of Beijing oil chicken
  • A method and application for quickly establishing the slow feather line of Beijing oil chicken
  • A method and application for quickly establishing the slow feather line of Beijing oil chicken

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The establishment of embodiment 1 method

[0040] 1.1 Design and screening of primers

[0041] According to the different sequences of the fast and slow feathers, primers were designed, and a total of 5 pairs of primers were designed. Corresponding to different genotypes, the copy numbers of different amplified fragments are different, as shown in Table 1 for details.

[0042] Table 1 Different primer names and copy numbers

[0043]

[0044]

[0045] Note: vim is located on chromosome 2 and is a known single-copy gene

[0046] The sequence corresponding to the Jun primer is the sequence fragment at the junction of the slow feather gene insertion repeat sequence and the original sequence

[0047] Firstly, the amplification efficiency of different primers was observed. The selected fluorescent PCR reaction program was pre-denaturation at 94°C for 3min, 15s at 94°C, annealing at 60°C for 30s, and a total of 40 cycles. The PCR reaction system was 20 μL, including 1...

Embodiment 2

[0071] Example 2 Establishment of Beijing Oil Fast and Slow Feather Lines

[0072] First, when the chicks are born, identify the feather speed and separate the fast feather type from the slow feather type. Individuals with uncertain feather speed types, that is, chicks with a difference of less than 2 mm in the length of the main wing feathers from the length of the main wing feathers, were directly eliminated.

[0073] Since the genotype and phenotype of fast-feather individuals correspond one-to-one, the establishment of fast-feather lines can be realized by using the isolated fast-feather cocks and fast-feather hens to mate and continuously select the population.

[0074] The genotype and phenotype of the slow-feather hen are consistent, but the genotype of the rooster is two, and the heterozygote cannot be stably inherited. Therefore, the key to breeding slow feather lines is the identification of rooster genotypes. Need to select genotype Z K Z K The slow-feather roos...

Embodiment 3

[0077] Example 3 Beijing Youji chicken male and female matching application

[0078] Utilize the slow feather line and the fast feather line population established in Example 2 to set up supporting production. Randomly select 30 fast-feather roosters, mix the semen, and inseminate 300 hens. Eggs are hatched 15 days after collection.

[0079] When 1786 chicks were hatched, senior judges were first invited to perform anal identification, and 971 roosters and 824 hens were obtained. Featherspeed patterns are then checked for roosters and hens. Theoretically, if a fast-feather rooster mates with a slow-feather hen, the offspring will be a hen with a fast-feather and a rooster with a slow-feather. The results of feather speed identification showed that there were 813 fast feathers and 11 slow feathers in 824 hens; 918 slow feathers and 53 fast feathers in 971 cocks.

[0080] In order to test the accuracy of feathering speed, 11 slow-feathering individuals in hens and 53 fast-fe...

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Abstract

The invention provides a new method for fast building a Beijing fatty chicken slow-feathering line. The method is characterized in that a specific primer pair is designed, a fluorescent quantitative PCR method is used to detect the copy number of Beijing fatty chicken Junction regions and PRLR genes, the feathering speed genotype of male Beijing fatty chickens can be identified fast, and the fatty chicken slow-feathering line can be built fast. The method is simple to operate, economical, capable of achieving large-scale sample detection, suitable for identifying the feathering speed genotype of the male Beijing fatty chickens, capable of fast building the Beijing fatty chicken slow-feathering line, and capable of achieving the self-differentiation matching of male and female Beijing fatty chickens.

Description

technical field [0001] The present invention relates to the field of poultry breeding, in particular to a method for identifying the genotype of rooster feather speed by means of molecular detection technology, and quickly cultivating the slow feather line of Beijing oil chicken, and its application in the production of Beijing oil chicken sexes . Background technique [0002] Beijing Youji, also known as "Chinese Palace Yellow Chicken", is a very good local breed native to the suburbs of Beijing. The chicken and eggs of Beijing oil chicken have good taste and high nutritional value. In recent years, they have been very popular, and the promotion scale and quantity have increased year by year (Zhang Jian, 2010). [0003] In the Beijing oil chicken breeding process, in order to facilitate management and increase group consistency, it is necessary to raise roosters and hens separately, or in order to save production costs, some farmers only raise chickens of one sex. Therefo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6888C12Q2600/124C12Q2531/113C12Q2563/107
Inventor 初芹张剑张尧王海宏耿爱莲刘华贵
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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