A method and application for quickly establishing the slow feather line of Beijing oil chicken
An oily and rapid technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of high cost, long time-consuming test cross test, etc. The effect of simple experimental design
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] The establishment of embodiment 1 method
[0040] 1.1 Design and screening of primers
[0041] According to the different sequences of the fast and slow feathers, primers were designed, and a total of 5 pairs of primers were designed. Corresponding to different genotypes, the copy numbers of different amplified fragments are different, as shown in Table 1 for details.
[0042] Table 1 Different primer names and copy numbers
[0043]
[0044]
[0045] Note: vim is located on chromosome 2 and is a known single-copy gene
[0046] The sequence corresponding to the Jun primer is the sequence fragment at the junction of the slow feather gene insertion repeat sequence and the original sequence
[0047] Firstly, the amplification efficiency of different primers was observed. The selected fluorescent PCR reaction program was pre-denaturation at 94°C for 3min, 15s at 94°C, annealing at 60°C for 30s, and a total of 40 cycles. The PCR reaction system was 20 μL, including 1...
Embodiment 2
[0071] Example 2 Establishment of Beijing Oil Fast and Slow Feather Lines
[0072] First, when the chicks are born, identify the feather speed and separate the fast feather type from the slow feather type. Individuals with uncertain feather speed types, that is, chicks with a difference of less than 2 mm in the length of the main wing feathers from the length of the main wing feathers, were directly eliminated.
[0073] Since the genotype and phenotype of fast-feather individuals correspond one-to-one, the establishment of fast-feather lines can be realized by using the isolated fast-feather cocks and fast-feather hens to mate and continuously select the population.
[0074] The genotype and phenotype of the slow-feather hen are consistent, but the genotype of the rooster is two, and the heterozygote cannot be stably inherited. Therefore, the key to breeding slow feather lines is the identification of rooster genotypes. Need to select genotype Z K Z K The slow-feather roos...
Embodiment 3
[0077] Example 3 Beijing Youji chicken male and female matching application
[0078] Utilize the slow feather line and the fast feather line population established in Example 2 to set up supporting production. Randomly select 30 fast-feather roosters, mix the semen, and inseminate 300 hens. Eggs are hatched 15 days after collection.
[0079] When 1786 chicks were hatched, senior judges were first invited to perform anal identification, and 971 roosters and 824 hens were obtained. Featherspeed patterns are then checked for roosters and hens. Theoretically, if a fast-feather rooster mates with a slow-feather hen, the offspring will be a hen with a fast-feather and a rooster with a slow-feather. The results of feather speed identification showed that there were 813 fast feathers and 11 slow feathers in 824 hens; 918 slow feathers and 53 fast feathers in 971 cocks.
[0080] In order to test the accuracy of feathering speed, 11 slow-feathering individuals in hens and 53 fast-fe...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com