Relative quantifying kit used for detecting copy number of survival genes of human motor neurons
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A motor neuron and gene copy number technology, applied in the determination/testing of microorganisms, biochemical equipment and methods, etc.
Inactive Publication Date: 2019-04-02
DEBIQI BIOTECH XIAMEN
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[0007] The object of the present invention aims at the inconvenience and insufficiency of prior art, provides a kind of relative quantification kit and its application of multiplex real-time fluorescent PCR method detection human motoneuron survival gene copy number
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Embodiment 1
[0068] 1. Nucleic acid extraction:
[0069] Twenty patients with a clinical diagnosis of SMA were included, as well as whole blood samples from 195 pregnant women. Use the nucleic acid extraction reagent produced by Dobtech Biotechnology (Xiamen) Co., Ltd. (record number: Minxia Machinery Equipment No. 20160080) to extract the whole blood sample collected by the EDTA anticoagulation tube, and use a micro-ultraviolet spectrophotometer to determine the nucleic acid after extraction Purity and concentration, the OD260 / OD280 should be between 1.6 and 2.0; dilute the genomic DNA concentration with sterilized double distilled water to 2ng / µL for later use.
[0070] 2. Serial dilution of the reference substance
[0071]Take 10 µL of the first control, the second control, and the third control, respectively, and add them to 3 centrifuge tubes, then add 40 µL 1×TE solution, and mix well to obtain 3 kinds of 5-fold diluted reference substances; For the reference substance, take out 10...
Embodiment 2
[0089] 1. Reagent specificity verification: cross-reaction of common clinical pathogens
[0090] 1. Experimental samples
[0091] Take 13 specific samples to verify the specificity of the reagent, hepatitis B virus, hepatitis C virus, herpes simplex virus type 1, herpes simplex virus type 2, Epstein-Barr virus, human cytomegalovirus, human herpes virus type 6A, Human herpesvirus type 6B, human parvovirus B19, varicella-zoster virus type B, adenovirus type 2, JC polyomavirus, BK polyomavirus.
[0092] 2. Experimental process
[0093] Use the first reaction solution, the second reaction solution and the third reaction solution to detect the above 13 specific samples respectively, analyze the test results, and verify the specificity of the reagents.
[0094] 3. Experimental results
[0095] The 13 specific samples tested by the three reaction solutions were all Undetermined, indicating good specificity and no cross-reaction. The specific results are shown in the table below: ...
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Abstract
The invention relates to the field of genetic testing, and specifically relates to a kit used for detecting the copy number of survival genes of human motor neurons. According to the kit used for detecting the copy number of the survival genes of the human motor neurons, a multifluorescent polymerase chain reaction (PCR) method is adopted for detecting the copy number of the survival genes of thehuman motor neurons; and specific primers and probes for exons 7 and 8 of SMN1 gene, exons 7 and 8 of SMN2 gene, exon 6 of SMN gene and internal reference gene cystic fibrosis transmembrane regulator(CFTR) are separately designed. The kit is smartly designed, so that, relative quantifying can be separately performed on the copy number of the exons 7 and 8 of the SMN1 gene, the exons 7 and 8 of the SMN2 gene as well as the exon 6 of the SMN gene by adopting 3 independent PCR reactions. The kit is easy to operate, strong in specificity and excellent in sensitivity. Thus, the result of the copynumber of the exon 6 of the SMN gene can be improved in terms of accuracy; so that, the kit is more suitable for clinical classifying of patients with spinal muscular atrophy and screening of carriers.
Description
technical field [0001] The invention relates to the field of gene detection, in particular to a kit for detecting the copy number of survival genes of human motor neurons. Background technique [0002] Spinal muscular atrophy (SMA) is a common autosomal recessive genetic disease caused by the human motor neuron survival gene 1 (Survival of MotorNeuron 1, SMN1) located on chromosome 5 (5q13) Large fragment deletions, transitions, or point mutations occur, of which more than 95% are large fragment deletions of exon 7 and / or exon 8; leading to progressive degeneration, symmetrical muscle weakness, atrophy and other symptoms. The neonatal incidence rate is 1 / 6,000~1 / 10,000, and the carrier rate in the normal population is 1 / 40~1 / 60. According to clinical symptoms and age of onset, SMA can be divided into four types, among which types I~III are called childhood SMA: SMA type I is the most serious subtype, and patients develop symptoms within 6 months after birth, and the general...
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