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Buckwheat extract abundant in inositol and general flavone and preparation method thereof

A technology of buckwheat extract and total flavonoids, which is applied in the field of buckwheat extract products, can solve the problems of high production cost, inability to produce on a large scale, and lack of comprehensive consideration of insulin resistance and synergistic research on related diseases

Inactive Publication Date: 2010-12-08
上海诺金科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low conversion rate of INOSITOL derivatives in buckwheat to INOSITOL products, and the high requirements for synthesizing INOSITOL by chemical methods, the production cost is too high to allow large-scale production
And most of the studies only study INOSITOL and buckwheat total flavonoids alone, without comprehensively considering the synergistic effect of these two functional ingredients on solving insulin resistance and related diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]Weigh 100 grams of mature buckwheat seeds, add an enzyme activator to soak for 2 hours, take them out at 25±2°C, and germinate under light conditions; collect the germinated buckwheat seeds, add 10 times the amount of 60% medicinal ethanol, pulverize, Boiling reflux extraction for 3 hours, filter out the extract; add a flocculant with a weight ratio of 10ppm - polymer chitosan, stir and let stand for 3 hours, filter, and separate the filtrate on a chromatographic column, the chromatographic column is AB -8 type macroporous adsorption resin, the eluent is 55% ethanol, the eluent is collected, vacuum concentrated to recover the ethanol, and then dried to obtain dry powder of buckwheat extract. The content of INOSITOL was detected by GC-MS, in which the content of D-chiro-inositol was 14.1%, and the content of Myo-inositol was 8.3%; the content of total flavonoids detected by UV method was 35.8%.

Embodiment 2

[0031] Weigh 100 grams of mature buckwheat seeds, add an enzyme activator to soak for 2 hours, take them out at 25±2°C, and germinate under light conditions; collect the germinated buckwheat seeds, add 10 times the amount of 60% medicinal ethanol, pulverize, Boiling reflux extraction for 3 hours, filter out the extract; add a flocculant with a weight ratio of 3ppm - polymer chitosan, stir and let stand for 3 hours, filter, and separate the filtrate on a chromatographic column. amide, the eluent is 50% ethanol, the eluent is collected, concentrated in vacuo to recover the ethanol, and then dried to obtain dry powder of buckwheat extract. The content of INOSITOL was detected by GC-MS, in which the content of D-chiro-inositol was 8.6% and the content of Myo-inositol was 8.7%; the content of total flavonoids was 32.8% detected by UV method.

Embodiment 3

[0033] Weigh 100 grams of mature buckwheat seeds, add an enzyme activator to soak for 2 hours, take them out at 25±2°C, and germinate under light conditions; collect the germinated buckwheat seeds, add 10 times the amount of 60% medicinal ethanol, pulverize, Boiling reflux extraction for 3 hours, filter out the extract; add a flocculant with a weight ratio of 30ppm - polymer chitosan, stir and let stand for 2 hours, filter, and separate the filtrate on a chromatographic column. amide, the eluent is 80% ethanol, the eluent is collected, concentrated in vacuo to recover the ethanol, and then dried to obtain dry powder of buckwheat extract. The content of INOSITOL was detected by GC-MS, in which the content of D-chiro-inositol was 19.6% and the content of Myo-inositol was 4.8%; the content of total flavonoids was detected by UV method was 40.8%.

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Abstract

The invention relates to a buckwheat extract abundant in inositol and general flavone and a preparation method thereof. The preparation method comprises the following steps of: (1) fully soaking a ripe buckwheat seed with a zymoexcitator and sprouting; (2) collecting the sprouted buckwheat seed, adding a certain amount of ethanol, pulverizing, refluxing, extracting, filtering and collecting seed extracting liquid; (3) adding a flocculant into the buckwheat seed extracting liquid, uniformly stirring, standing for adsorbing; (4) filtering and removing the flocculant and carrying out column chromatography separation on filter liquid, wherein a chromatographic column is macroporous absorption resin; and eluting, collecting an eluent, concentrating for recycling the ethanol and drying to obtain the buckwheat extract. The buckwheat extract contains effectively concentrated inositol and general flavone substances; and by synergy, the buckwheat extract can be applied to the preparation of foods, health-care foods and medicaments for curing, preventing or assisting to cure diseases caused by body insulin resistance, such as diabetes, hypertension, hyperlipidemia, PCOS (Polycystic Ovary Syndrome), and the like.

Description

Technical field: [0001] The present invention relates to the technical field of medicine health care. Specifically, it relates to a buckwheat extract product capable of preventing and treating diabetes, hypertension, hyperlipidemia, polycystic ovary syndrome (PCOS), and diseases caused by the body's insulin resistance. Background technique: [0002] Chinese folk proverbs have the sayings that "there are many kinds of crops, and the crop is the largest" and "the cultivation of grains is the first". There are many records about the prevention and treatment of buckwheat in ancient Chinese books. "Qi Min Yao Shu" has the record that "people who are afraid of the wind and cold use noodle soup and flour as cakes, which make the buckwheat sweat even after decades." ; "The Classic of Materia Medica" has the record of "reinforcing the stomach and nourishing the energy"; "Compendium of Materia Medica" has the discussion of "lowering the qi, widening the intestines and grinding stagna...

Claims

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Application Information

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IPC IPC(8): A61K36/70A61P3/06A61P3/10A61P9/12A61P15/00A23L1/30A61K131/00A23L33/105
Inventor 夏涛刘华奇吴芬宏陈丹青
Owner 上海诺金科生物科技有限公司
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