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Method for extracting chondroitin, collagen and high-calcium powder from animal cartilage

A collagen and chondroitin technology, applied in the fields of chondroitin, extraction of high calcium powder, and collagen, can solve problems such as environmental pollution, waste of protein resources, etc., and achieve reduced acid-base consumption, good economic and social benefits, and comprehensive pollution index. falling effect

Inactive Publication Date: 2012-06-27
QINGDAO BETTER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The method of extracting chondroitin in the prior art is to destroy the protein with strong alkali and high temperature, and then use alcohol precipitation to separate the chondroitin. The protein destroyed by strong alkali is discharged with waste water, which not only wastes the precious protein in animal cartilage resources and cause serious environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Simply wash 1 ton of fresh bovine cartilage (including costal cartilage and throat) and break it into pieces of 5-20mm, mix it with water at a ratio of 1:1, add it to the enamel reaction kettle, heat it to 100°C and keep it for 1-2 hour, ensure that the bones are cooked to a certain degree, then pass cold water into the jacket of the reaction kettle to cool down, and when the temperature is lowered to 40-50°C, add 0.01%--0.03% collagenase by weight of cartilage (dried) and add 0.1%- 0.3% papain, keep the temperature at 40-60°C, stir and hydrolyze for 8 hours, coarsely filter out the bone residue, after the bone residue is washed, dried and crushed, about 200 kg of high calcium powder is obtained. The coarsely filtered liquid is then finely filtered to obtain a clear fine filtrate, which is added with more than 90% alcohol to an alcohol mass percentage concentration of 60-70%, and the chondroitin crystals are precipitated. The liquid is a mixed solution of collagen and a...

Embodiment 2

[0035] Crush 1 kg of dried shark skulls into 5-20mm pieces, add water at a ratio of 1:4, heat to 100°C, keep for 1-2 hours, then cool down to 40-60°C, add cartilage (break dry) 0.01%-0.03% collagenase plus 0.1%-0.3% papain by weight, keep the temperature at 40-60°C, stir and hydrolyze for 8 hours, filter out the bone residue with 100 mesh, wash, dry and crush the bone residue Finally, about 300 grams of high-calcium powder were obtained; the liquid filtered out was finely filtered to obtain a clear fine filtrate, and more than 90% alcohol was added to the liquid until the concentration of alcohol mass percentage was 60-70%, the chondroitin crystals precipitated, and the liquid was collagen A mixed solution of protein and alcohol, the chondroitin crystals were washed with more than 90% alcohol, and dried to obtain 120 grams of chondroitin powder; the mixed solution was distilled to recover alcohol and then it was a collagen solution, which was concentrated to 70% concentration t...

Embodiment 3

[0037] The steps and methods are the same as in Examples 1 and 2, except that the dried pig nose bone is used as a raw material, and the pig nose bone contains about 40% chondroitin, which can be extracted with this technology, and the yield can reach 35% (the finished product has a purity of 90% as detected by the CPC method). %), proving that the present invention co-produces chondroitin while producing collagen, and its yield, product purity and appearance all reach or exceed the level of single production of chondroitin.

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PUM

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Abstract

The invention discloses a method for extracting chondroitin, collagen and high-calcium powder from animal cartilages, comprising the following steps of: a, chopping the cleaned animal cartilages into pieces, adding water for mixing, placing into a reaction still, and heating to denature protein; b, introducing cold water into a jacket of the reaction still for cooling, adding collagenase and papain till the internal temperature of the reaction still is 30-60 DEG C, keeping the temperature of 30-70 DEG C and the PH value of 7-8, and hydrolyzing under stirring; c, roughly filtering a hydrolyzedsolution to obtain cartilage residues containing calcium phosphate; d, finely filtering roughly-filtered liquid by a filter press to obtain clear fine filtrate, and adding ethanol to the fine filter liquor to precipitate chondroitin crystals; and e, after the chondroitin crystals are precipitated, obtaining a mixed solution of the collagen and the ethanol. The invention overcomes the defects of serious pollution and only single product production in the traditional process, enhances the output value rate by more than 30 percent and reduces the comprehensive pollution index of chondroitin production by more than 95 percent.

Description

technical field [0001] The invention relates to the technical field of animal cartilage processing, in particular to a method for decomposing animal cartilage by enzymatic hydrolysis and extracting high-calcium powder, chondroitin and collagen from the enzymatic decomposition products. Background technique [0002] Animal cartilage, including cartilage of terrestrial animals and aquatic animals, is now mainly used to extract chondroitin, while animal cartilage contains chondroitin, and also contains about 5-10 times as much protein as chondroitin (mainly collagen), 3-5 times calcium salt. [0003] The method of extracting chondroitin in the prior art is to destroy the protein with strong alkali and high temperature, and then use alcohol precipitation to separate the chondroitin. The protein destroyed by strong alkali is discharged with waste water, which not only wastes the precious protein in animal cartilage resources and cause serious environmental pollution. Contents ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B25/32C12P21/06A61K35/32A61K33/42A61P3/14A61K35/60
Inventor 曹荣军
Owner QINGDAO BETTER BIO TECH
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