Method for preparing microorganism agent for increasing production of oilfield microorganisms or protecting environment

A microbial inoculum and microorganism technology, which is applied in the preparation of microbial inoculum powder products and the field of preparation of microbial solid inoculants, can solve the problems of difficulty in guaranteeing microbial activity, large injection amount, and difficulty in realization.

Inactive Publication Date: 2010-12-15
DALIAN BITEOMICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process not only increases the cost of microbial oil recovery, but is also difficult to achieve in practical applications
However, if the pre-produced microbial fermentation liquid or sludge is transported to the oil field for construction, it is difficult to control the temperature, dissolved oxygen and other conditions during the transportation, and the activity of the microorganisms will be difficult to guarantee. Shipping costs will also be significantly higher

Method used

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  • Method for preparing microorganism agent for increasing production of oilfield microorganisms or protecting environment
  • Method for preparing microorganism agent for increasing production of oilfield microorganisms or protecting environment
  • Method for preparing microorganism agent for increasing production of oilfield microorganisms or protecting environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of microbial agent based on a single strain

[0037] The target microorganism selected in this example is a strain of Bacillus subtilis (ATCC No. 21332) that produces surfactants, which can be used for microbial flooding in oil production engineering.

[0038] 1. Medium: select LB medium, autoclave at 121°C for 20 minutes, and use it for later use;

[0039] The LB medium is composed of 1% peptone, 0.5% yeast powder, 0.5% sodium chloride, and the balance is water, and has a natural pH value.

[0040] 2. Shake flask culture: inoculate the target bacteria into LB liquid medium, and carry out shaking flask culture. The conditions are: temperature 50℃, rotation speed 150rpm, 30% liquid volume in triangular flask, and cultivation time 12h;

[0041] 3. Fermentation in the seed tank, transfer the shake flask liquid of the target bacteria into the seed tank according to the inoculum of 2%, and carry out fermentation culture. The conditions are: tank temperature 50℃,...

Embodiment 2

[0053] Example 2: Preparation of microbial agents based on mixed strains

[0054] The target microorganism selected in this example is a complex of Bacillus subtilis (ATCC No. 21332), Bacillus licheniformis (ATCC No. 39307), and Pseudomonas aeruginosa (ATCC No. 9027). This complex bacteria has a certain The ability to degrade xanthan gum.

[0055] 1. Medium: select LB medium, autoclave at 121°C for 20 minutes, and use it for later use;

[0056] The LB medium is composed of 0.8% peptone, 0.3% yeast powder, 0.5% sodium chloride, and the balance is water, with a natural pH value.

[0057] 2. Shake flask culture: inoculate the target bacteria into LB liquid medium, and carry out shaking flask culture. The conditions are: temperature 37℃, rotation speed 160rpm, volume of liquid in triangular flask 20%, culture time 12h;

[0058] 3. Fermentation in the seed tank, transfer the shake flask liquid of the target bacteria into the seed tank according to the inoculum of 3%, and carry out the ferme...

Embodiment 3

[0070] Example 3: Preparation of microbial agents based on mixed strains

[0071] The target microorganisms selected in this example are a complex of Bacillus subtilis (ATCC NO. 21332), Bacillus licheniformis (ATCC NO. 39307), and Arthrobacter paraffineus (ATCC NO. 19558) This compound bacteria has a certain ability to produce biopolymers and can be used for profile control and water shutoff in the process of oil exploitation.

[0072] 1. Medium: select glucose 2% + basic inorganic salt medium, autoclave at 115°C for 15 minutes, and use it for later use;

[0073] The LB medium is composed of 1% peptone, 0.5% yeast powder, 0.5% sodium chloride, the balance is water, and the pH value is 6.0.

[0074] 2. Shake flask culture: inoculate the target bacteria into the pre-prepared liquid medium, and carry out shaking flask culture. The conditions are: temperature 60℃, rotation speed 160rpm, volume of liquid in triangular flask 20%, culture time 12h;

[0075] 3. Fermentation in the seed tank, t...

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Abstract

The invention discloses a method for preparing a microorganism agent for increasing production of oilfield microorganisms or protecting the environment. A composite microorganism agent is prepared by compounding microorganism sludge obtained by centrifugal sedimentation of microorganism fermentation liquor and a protecting agent and drying the compound; and every one gram of microorganism agent totally comprises 1*109 to 1*1,011 living microorganisms. In the method, powder with high activation rate is prepared from single microorganism or complex microorganism, so the aims of prolonging the product storage time, saving the transportation cost and simplifying the using method are fulfilled. The method for preparing the agent has the characteristics of simple operation, convenient use, wide application range for microorganisms, low cost and suitability for laboratories and batch production.

Description

Technical field [0001] The present invention belongs to the technical field of microbial oil extraction, and mainly relates to a preparation method of microbial powder products used for oil and gas field microbial production increase or environmental protection purposes, and more specifically for the microbes used for oil field production increase or environmental protection, using three different protections , To prepare the technology of microbial solid inoculum and its application range. Background technique [0002] Microbial enhanced oil recovery technology (MEOR technology) is a kind of biosurfactant, gas, polymer, organic acid, organic ketone, organic ketone, organic ketone, organic A method of using esters and other substances to improve oil recovery. Since Beckman proposed that bacteria can extract oil in 1926, microbial wax removal and heavy oil viscosity reduction, microbial selective sealing of formations, microbial huff and puff, and microbial enhanced water floodin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C09K8/582C12R1/125C12R1/10C12R1/06C12R1/09C12R1/01C12R1/225C12R1/085C12R1/385
Inventor 梅晓丹刘滢赵静王平陈琛付步飞杨平杜国丰刘文静刘润海魏子超
Owner DALIAN BITEOMICS INC
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