PCR marker linked with wheat powdery mildew resistant genes PmLK906 and using method thereof

Abstract

The invention relates to a PCR marker linked with wheat powdery mildew resistant genes PmLK906 and a using method thereof and belongs to the technical field of plant molecular breeding. The marker is named as Xgdm93-122, is a pair of amplimers consisting of a nucleotide sequence represented by SEQ ID No. 1 and a nucleotide sequence represented by SEQ ID No. 2. The using method comprises the following steps of: performing PCR amplification on primers of Xgdm93-122 by taking genomic DNA of wheat to be detected as a template; and detecting whether an amplified product contains 122bp fragment specific bands, wherein an individual plant which can amplify the 122bp fragment specific bands carries the disease-resistant genes PmLK906, and an individual plant which cannot amplify the 122bp fragment specific bands does not carry the disease-resistant genes PmLK906. The molecular marker can accurately detect the PmLK906 genes in different wheat genetic backgrounds. The technology can accelerate the transformation velocity from the PmLK906 genes to wheat production varieties.

Description

technical field [0001] The invention belongs to the technical field of plant molecular breeding, in particular to a PCR marker linked with wheat powdery mildew resistance gene PmLK906 and its usage. Background technique [0002] Wheat is the second largest grain crop in the world and the most important crop in my country. The annual sowing area in Henan Province alone reaches more than 70 million mu. Diseases are one of the main reasons for the reduction of wheat production. Wheat rust, sheath blight, scab, smut, and yellow dwarf all occur in the Huanghuai wheat region, while powdery mildew is the earliest onset and the longest duration. The disease with the most long-term growth and the most serious harm has a wide range of incidence and serious harm, and it is on the rise. In 1990, the incidence of powdery mildew reached 18 million ha 2 , accounting for about 1 / 2 of my country's wheat area, 3.6 times that of 1981. Generally, powdery mildew can cause a yield loss of 13% ...

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Problems solved by technology

However, the varieties popularized in a large area are all susceptible to powdery mildew, and the prevalence of powdery mildew cannot be controlled.

[0003] Although medicines have a certain control effect on wheat powdery mildew, there are the following deficiencies. Firstly, medicine control cannot stop the epidemic; secondly, the onset period of powdery mildew is short, only 5 to 7 days. During the epidemic period of wheat powdery mildew, the disease should be controlled The degree needs to spray drugs 3 to 4 times; the third is that drug control increases the cost, and according to 3 spraying, it will increase manpower and drug investment by about 50 yuan per mu; the fourth is that drug control affects the quality of wheat; the fifth is that drug residues are not enough Conducive to environmental protection and green production of wheat

In 2009, the survey results in the northern Henan wheat area showed that the effect of drugs on powdery mildew control declined

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