Gene chip for detecting pancreatic cancer and application thereof
A gene chip and detection chip technology, which is applied in the field of gene chips for detecting pancreatic cancer, can solve problems such as poor results, and achieve the effects of improving the cure rate, reducing the mortality rate, and having a good clinical application prospect.
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Embodiment 3
[0050] Embodiment 3 chip hybridization experiment:
[0051] 1. Total RNA extraction (TRIzol method):
[0052] Add 1ml Trizol (purchased from SIGMA Company) per 100mg of tissue, and use a homogenizer to fully break up. Add about 1 / 5 volume of chloroform, mix thoroughly and let stand at room temperature for 5 minutes. After centrifugation at 12,000rpm at 4°C for 15 minutes, transfer the supernatant to a new 1.5ml centrifuge tube, add an equal volume of isopropanol, gently invert and mix, and let stand at room temperature for 5 minutes. After centrifugation at 12000 rpm at 4°C for 10 minutes, the supernatant was removed, 2 / 5 volume of 70% ethanol was added to the precipitate, and the precipitate was washed by centrifugation at 12000 rpm at 4°C for 15 minutes. Remove the supernatant, dry the precipitate at room temperature, add an appropriate amount of RNase-free water to fully dissolve the precipitate, and measure the OD260 and OD280 values.
[0053] 2. Total RNA quality detec...
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