Composite fat granule and preparation method thereof

A fat granule and fat technology is applied in the field of compound fat granule and its preparation, which can solve the problems of reducing the absorption rate of transplantation, increasing the absorption rate, fibrosis, etc., so as to improve the survival rate of fat cells, promote the regeneration of adipose tissue, and improve the local tissue. effect of ischemia

Active Publication Date: 2013-04-24
成都世联康健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of the transplanted fat cells have been damaged or necrotic before transplantation; the local aseptic inflammatory reaction after transplantation leads to fat cell necrosis, and tissue shrinkage caused by leaking lipid fibrosis; secondary to fat cell lipid loss or Dedifferentiated non-necrotic volume reduction, etc., can cause low survival rate and high absorption rate of transplanted fat cells, and the absorption rate can reach more than 60% within one year, and if the transplanted fat volume is too large, it is difficult to rebuild the blood supply. Reaching the center of the graft will also lead to necrosis and liquefaction of the center of the grafted fat particles, increasing the absorption rate, and even fibrosis, resulting in hardening of the grafted area
[0004] At present, in order to improve the survival rate of transplanted fat, some scholars add a certain amount of adipose stem cells to the transplanted simple fat granules to reduce the absorption rate of transplantation, and have achieved certain results; condition, resulting in hard lumps in the planted area

Method used

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  • Composite fat granule and preparation method thereof
  • Composite fat granule and preparation method thereof
  • Composite fat granule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The composite fat particles of this embodiment (referred to as PA fat) are composed of platelet-rich plasma, human thrombin solution, fat stem cells, and fat particles according to the following ratios:

[0042] 2ml platelet-rich plasma: 1ml human thrombin solution: (2±0.5)×10 7 adipose stem cells: 4ml fat particles;

[0043] in:

[0044] The platelet concentration in platelet-rich plasma is (1300.00±400.00)×10 9 / L;

[0045] The component ratio of the human thrombin solution is: 1ml distilled water: 0.1g calcium chloride: 1000 units human thrombin freeze-dried powder.

[0046] Present embodiment PA fat is made by the method comprising the following steps:

[0047] (1), preparation of platelet-rich plasma (PRP):

[0048] Anticoagulated whole blood was extracted, and platelet-rich plasma (PRP) was obtained by two centrifugation methods as follows:

[0049] With a 5ml syringe pre-filled with 0.5ml 3.8% trisodium citrate anticoagulant, extract 4.5ml whole blood, shak...

Embodiment 2

[0060] The composite fat particles of this embodiment (referred to as PA fat) are composed of platelet-rich plasma, human thrombin solution, fat stem cells, and fat particles according to the following ratios:

[0061] 2ml platelet-rich plasma: 1ml human thrombin solution: (2±0.5)×10 7 adipose stem cells: 4ml fat particles;

[0062] in:

[0063] The platelet concentration in platelet-rich plasma is (1300.00±400.00)×10 9 / L;

[0064] The component ratio of the human thrombin solution is: 1ml distilled water: 0.1g calcium chloride: 800 units human thrombin freeze-dried powder.

[0065] Present embodiment PA fat is made by the method comprising the following steps:

[0066] (1), preparation of platelet-rich plasma (PRP):

[0067] Anticoagulated whole blood was extracted, and platelet-rich plasma (PRP) was obtained by two centrifugation methods as follows:

[0068] With a 5ml syringe pre-filled with 0.5ml 3.8% trisodium citrate anticoagulant, extract 4.5ml whole blood, shake...

Embodiment 3

[0079] The composite fat particles of this embodiment (referred to as PA fat) are composed of platelet-rich plasma, human thrombin solution, fat stem cells, and fat particles according to the following ratios:

[0080] 2ml platelet-rich plasma: 1ml human thrombin solution: (2±0.5)×10 7 adipose stem cells: 4ml fat particles;

[0081] in:

[0082] The platelet concentration in platelet-rich plasma is (1300.00±400.00)×10 9 / L;

[0083] The component ratio of the human thrombin solution is: 1ml distilled water: 0.1g calcium chloride: 1200 units human thrombin freeze-dried powder.

[0084] Present embodiment PA fat is made by the method comprising the following steps:

[0085] (1), preparation of platelet-rich plasma (PRP):

[0086] Anticoagulated whole blood was extracted, and platelet-rich plasma (PRP) was obtained by two centrifugation methods as follows:

[0087] With a 5ml syringe pre-filled with 0.5ml 3.8% trisodium citrate anticoagulant, extract 4.5ml whole blood, shak...

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Abstract

The invention discloses a composite fat granule and a preparation method thereof. The composite fat granule comprises the components in the following proportions: 2ml of platelet-rich plasma, 1ml of human thrombin solution, (2+ / -0.5)*107 fat adipose-derived stem cells and 4ml of fat granules. Through the composite fat granule, the survival rate of transplanted fat can be improved, the long-term absorption rate is reduced, and situations, such as the formation of ectopic fibrotic nodules and the like can not occur.

Description

technical field [0001] The invention belongs to the technical field of transplant materials, in particular to a composite fat particle for transplant and a preparation method thereof. Background technique [0002] In 1893, Neuber completed the first case of fat grafting using multiple autologous free small fat blocks to fill soft tissue defects and achieved satisfactory results. Since then, fat grafting has gradually become popular. Especially after liquid silicone injection was banned due to many complications, people conducted a lot of exploration in the field of transplant materials. By the early 1980s, with the maturity and development of liposuction technology, autologous free fat transplantation was once again launched. upsurge. Because of the advantages of abundant autologous fat sources, convenient sampling, safety and reliability, and no immune rejection, it is easily accepted by patients. And for those with obvious fat accumulation all over the body, autologous f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/54
Inventor 田卫东郭丽娟汤炜王杭龙洁刘磊
Owner 成都世联康健生物科技有限公司
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