Compound microbial agent for treating dye waste water as well as preparation and application of compound microbial inoculum

A technology for compounding microbial inoculants and dye wastewater, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of inability to decolorize multiple dyes, large limitations of decolorization and degradation, and inability to adapt to changes in dye species. Water quality fluctuations problems, such as low cost, high adaptability, and easy compounding

Inactive Publication Date: 2012-11-21
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the decolorization and degradation of dyes by a single strain has great limitations and strong specificity. It cannot decolorize a variety of dyes, and cannot adapt to the characteristics of large changes in dye types and strong fluctuations in water quality in the printing and dyeing industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 6.0-8.0, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activation solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans are respectively 12%: 12%: 12%: 24%: 20%: 20% by volume Mix to obtain the desired compound microbial agent.

[0030] 1. It is used to treat reactive red dye wastewater. The initial dye concentration is 60mg / L, and the chromaticity and absorbance value represent 0.952. The dye reactive red 195 is an azo dye with a complex structure, and it is very difficult to treat it with a single microbial age...

Embodiment 2

[0034] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 7.0-7.2, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activating solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans is mixed by volume percentage 14%: 28%: 21%: 7%: 17.5%: 12.5%. Obtain the desired compound microbial bacterial agent.

[0035] 1. Take 1000mL of reactive red 195 dye wastewater with an initial dye concentration of 60.00mg / L and a chromaticity absorbance of 0.952, add glucose 1.5g, sodium chloride 1g, potassium dihydrogen phosphate 2.65g, disodium hydrogen phosphate 4.26g, and MgSO 4 ·7H 2 O 0...

Embodiment 3

[0038] Acinetobacter haemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans were respectively cultured in beef extract peptone medium LB for 18-24 hours, The culture temperature is 20-37°C, the pH of the culture medium is 7.0-7.2, and the OD 600 0.6±0.05 activation solution of Acinetobacter hemolyticus, Escherichia coli, Bacillus curvedus, Yersinia intermedia, Enterobacter lutherii, Klebsiella nigerii; The activating solution of Helicobacter, Bacillus flexus, Yersinia intermedia, Enterobacter rudii, and Klebsiella nigricans is mixed by volume percentage 28%: 14%: 21%: 7%: 17.5%: 12.5%. Obtain the desired compound microbial bacterial agent.

[0039] 1. Take 1000 mL of malachite green dye wastewater with an initial dye concentration of 80 mg / L and a chromaticity absorbance value of 1.629, add 1.5 g of glucose, 1 g of sodium chloride, 2.65 g of potassium dihydrogen phosphate, and 4.26 g of disodium hydrogen phosphate,...

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Abstract

The invention discloses a compound microbial agent for treating dye waste water as well as preparation and application of the compound microbial agent. The compound microbial agent is formed by complexing acinetobacter haemolyticus, Escherichia coli, bacillus flexus, Yersinia intermedia, luther enterobacter and Klebsiella pneumoniae. Complexing is simple and convenient, the broad spectrum is strong, the difficulty that a single microbial agent can not meet the requirement of water quality variation is overcome, and the cost of treating the dye industrial waste water is reduced.

Description

technical field [0001] The invention relates to the field of dye and printing and dyeing industrial wastewater treatment, in particular to a composite microbial agent for treating dye wastewater, its preparation and application. Background technique [0002] Dye production and printing and dyeing wastewater has high content of organic pollutants, large changes in water quality, poor biodegradability, and large chroma. The treatment of dye wastewater has become an urgent problem in today's environmental science community. At present, the chromaticity removal of dyes mainly uses chemical methods and biological decolorization methods. The use of chemical methods has been restricted due to reasons such as cost and water quality changes. At present, the printing and dyeing industry is mostly order production, the variety changes quickly, and the types of dyes vary greatly. Therefore, traditional biological methods and single-type microbial preparations often cannot meet the requ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/19C12R1/07C12R1/22
Inventor周元祥叶红曼石倩倩曾庆龙
OwnerHEFEI UNIV OF TECH