Detection method of xylooligosaccharide

A technology of xylo-oligosaccharide and detection method, applied in the field of small polymers, can solve the problems of low detection sensitivity, poor separation effect, detection error and the like, and achieve high detection sensitivity, good separation effect and good detection reproducibility Effect

Inactive Publication Date: 2011-09-07
SUZHOU XIANKUO BIOTECH +1
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Problems solved by technology

However, the existing high-performance liquid chromatography detection method cannot clearly separate the chromatographic peaks of each component, the detection sensitivity is not high, and it cannot accurately determ

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  • Detection method of xylooligosaccharide
  • Detection method of xylooligosaccharide

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[0018] The analysis and detection method according to a preferred embodiment of the present invention is as follows:

[0019] Test sample: The xylo-oligosaccharide standard is dissolved in water, and its concentration is 5-10 mg / mL;

[0020] Chromatographic conditions: The high performance liquid chromatography column used was: Sepax Proteomix SAX-NP5 (5um, 4.6x150mm, SN: X101201), the column temperature was 35 °C, the injection volume was 10 μL, and the evaporative light scattering detector (ELSD) , the drift tube temperature was 100 °C, and the carrier gas flow rate was 2.0 mL / min.

[0021] from figure 1 and figure 2 It can be seen from the data in Table 1 to Table 3 that the detection method of the present invention has high detection sensitivity, and can accurately quantitatively measure the small polymer components in xylo-oligosaccharides, with good separation effect and repeatable detection. good sex.

[0022] The above-mentioned embodiments are only intended to il...

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Abstract

The invention discloses a detection method of xylooligosaccharide. According to the method, a high-performance liquid chromatograph and an evaporative light scattering detector are used for performing qualitative and quantitative detection on small polymers, with different degrees of polymerization, in the xylooligosaccharide. The method is very high in detection sensitivity and can quantitatively detect the small polymer components in the xylooligosaccharide accurately; the separating effect is good; and the detecting repeatability is good.

Description

technical field [0001] The invention relates to a method for qualitatively or quantitatively detecting small polymers of different polymerization degrees in xylooligosaccharides. Background technique [0002] Xylo-oligosaccharides refer to compounds containing 2-7 glycosidic bonds polymerized, which are often covalently bound to proteins or lipids and exist in the form of glycoproteins or glycolipids. Xylo-oligosaccharides usually form small polymers by linking 2 to 4 monosaccharides through glycosidic bonds. This type of xylo-oligosaccharides is difficult to be digested and absorbed by the gastrointestinal tract, has low sweetness, low calories, and basically does not increase blood sugar and blood lipids. Xylo-oligosaccharides cannot be destroyed by the stomach acid of the human body, nor can it be broken down by digestive enzymes. But it can be fermented by bacteria in the intestine and converted into short-chain fatty acids and lactic acid. [0003] At present, xylooli...

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Application Information

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IPC IPC(8): G01N30/74G01N30/34
Inventor 李彧娜崔爱艳黄学英
Owner SUZHOU XIANKUO BIOTECH
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