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Method for detecting mercury ion concentration of water sample

A technology of mercury ion and concentration, which is applied in the direction of using substance absorption and weighing, and can solve the problem of high detection cost

Inactive Publication Date: 2012-11-07
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are highly selective, but still need to be labeled, or use expensive and bulky instruments such as fluorescence instruments and UV-Vis spectrophotometers, and the detection cost is still high

Method used

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  • Method for detecting mercury ion concentration of water sample
  • Method for detecting mercury ion concentration of water sample
  • Method for detecting mercury ion concentration of water sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, application method of the present invention detects mercury ion concentration (adopting the probe probe-1 of different concentrations)

[0053] 1. Adsorption of probe-1 by QCM quartz plate

[0054] 1. Pretreatment of QCM quartz wafers

[0055] QCM quartz slices are washed with newly configured Piranha (by 70 parts by volume 98% H 2 SO 4 aqueous solution and 30 volume parts H 2 o 2 Composition) wash for 2 minutes, then use a resistivity of 18.2M Q cm -1 Wash thoroughly with pure water, then wash with ethanol and acetone in turn, then wash with pure water, and finally blow dry with nitrogen.

[0056] 2. Adsorption of probe-1 by QCM quartz plate

[0057] Add 10 μL solution A (solution A1, solution A2, solution A3 or solution A4) dropwise on the gold electrode on the surface of the pretreated QCM quartz plate, and adsorb at room temperature for 2 hours; wash it with pure water and dry it with nitrogen; add dropwise 50 μL containing 1mM ethanol solution ...

Embodiment 2

[0068] Embodiment 2, application method of the present invention detects the sensitivity of mercury ion concentration (the particle diameter of gold particle is about 12nm)

[0069] 1. Adsorption of probe-1 by QCM quartz plate

[0070] 1. Pretreatment of QCM quartz wafers

[0071] Same as step 1 of embodiment 1.

[0072] 2. Adsorption of probe-1 by QCM quartz plate

[0073] Add 10 μL solution A3 (the concentration of probe-1 is 2 μM) dropwise on the gold electrode on the surface of the pretreated QCM quartz plate, and adsorb at room temperature for 2 hours; wash with pure water and blow dry with nitrogen; add dropwise 50 μL solution containing 1 mM 6 - Ethanol solution of mercapto-1-hexanol, adsorbed at room temperature for 1 h; washed with ethanol and water in sequence, and dried with nitrogen to obtain a QCM quartz plate modified with probe-1.

[0074] 2. Adsorption of gold nanoparticles to probe-3

[0075] 1. Preparation of gold particle sol

[0076] Same as step 2 of em...

Embodiment 3

[0085] Embodiment 3, application method of the present invention detects mercury ion concentration (the particle diameter of gold particle is about 65nm)

[0086] 1. Adsorption of probe-1 by QCM quartz plate

[0087] 1. Pretreatment of QCM quartz wafers

[0088] Same as step 1 of embodiment 1.

[0089] 2. Adsorption of probe-1 by QCM quartz plate

[0090] Same as step 1 of embodiment 2.

[0091] 2. Adsorption of gold nanoparticles to probe-3

[0092] Prepare 30mL of 1% (g / 100mL) chloroauric acid aqueous solution, heat to reflux, quickly add 0.16mL of 1% (g / 100mL) trisodium citrate aqueous solution under stirring, continue heating for 15min and leave to cool to room temperature to obtain gold particle sol . The scanning electron micrograph of the gold particle sol is shown in Figure 6 , it can be seen that the particle size of the gold particles in the gold particle sol is about 65nm.

[0093] 2. Adsorption of gold nanoparticles to probe-3

[0094] Same as step 2 of em...

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Abstract

The invention discloses a method for detecting mercury ion concentration of a water sample. The method comprises the following steps of: (1) mixing the water sample to be tested and solution B, hybridizing the mixture with a single chain DNA probe A crosslinked on a gold electrode of a quartz crystal microbalance (QCM) quartz plate, and recording reading a of the QCM quartz plate, wherein the solution b is the solution comprising a single chain DNA probe B and the QCM quartz plate is a gaseous phase type QCM adopting the gold electrode; (2) hybridizing the hybridized QCM quartz plate obtainedin the step (1) with a single chain DNA probe C crosslinked on nano gold particles and recording reading b of the QCM quartz plate; (3) calculating frequency response of the QCM quartz plate, whereinthe frequency response of the QCM quartz plate is (a-b)Hz; and (4) evaluating the mercury concentration of the solution to be tested according to the frequency response of the QCM quartz plate. The method can be used for detecting the mercury concentration of drinking water, and requires very small quantity of samples to be detected, is simple in operation, low in cost and suitable for popularization and the instruments are portable.

Description

technical field [0001] The invention relates to a method for detecting mercury ion concentration in water samples. Background technique [0002] A Quartz Crystal Microbalance (QCM) is a sensor made using the piezoelectric properties of a quartz crystal resonator. It converts the surface quality change of the quartz crystal oscillator electrode into the frequency change of the output electrical signal of the quartz crystal oscillator circuit, and then obtains high-precision data through other auxiliary equipment such as a computer. The Sauerbrey equation is the main theoretical basis for piezoelectric mass effect sensing. Its development makes quartz crystal microbalances widely used as mass sensors, and the detection sensitivity can reach ng level. [0003] DNA oligonucleotides have been widely used in many fields as a method for specific recognition of mercury ions as probes. Studies have shown that thymine (T) can be coupled through coordination with mercury ions in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/02
Inventor 江龙盛仲翰韩建华张建平
Owner INST OF CHEM CHINESE ACAD OF SCI
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