Amiopyrimidine-modified gold nano particles and preparation method and use thereof

A gold nanoparticle and aminopyrimidine technology, which is applied to the gold nanoparticles modified by aminopyrimidine and the fields of preparation and use thereof, can solve the problems of nanoparticle agglomeration and inability to store, and achieves small particle size distribution, simple preparation method, and excellent antibacterial properties. active effect

Active Publication Date: 2011-09-21
重庆龙翼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Selvaraj, V. et al. (Selvaraj, V.; Alagara, M.; Hamerton, I.; Analytical detection and biological assay of antileukemic drug using gold nanoparticles. Electrochimica Acta 2006, 52, 1152-1160) combined drug 6-mercaptopurine with gold After the nanoparticles are mixed, 6-mercaptopurine-modified gold nanoparticles are obtained, and the drug efficacy is improved, but the nanoparticles are seriously agglomerated and cannot be preserved

Method used

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  • Amiopyrimidine-modified gold nano particles and preparation method and use thereof
  • Amiopyrimidine-modified gold nano particles and preparation method and use thereof
  • Amiopyrimidine-modified gold nano particles and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of gold nanoparticles modified by 2-mercapto-4,6-diaminopyrimidine, the steps are:

[0062] (1) Prepare reaction solution:

[0063] Preparation concentration is the dimethyl sulfoxide solution of chloroauric acid 5mM, is about to dissolve 41.2mg chloroauric acid solid in 20ml dimethyl sulfoxide and make;

[0064]Prepare a 2-mercapto-4,6-diaminopyrimidine dimethyl sulfoxide solution with a concentration of 10 mM, that is, add 14.2 mg of 2-mercapto-4,6-diaminopyrimidine solid to 10 ml of methanol, and add 50 μl of hydrochloric acid (0.1 N) and 50 μl Triton X-100, ultrasonically dissolved to obtain a mixed solution containing 2-mercapto-4,6-diaminopyrimidine;

[0065] Prepare a sodium borohydride dimethyl sulfoxide solution with a concentration of 60 mM, which is prepared by dissolving 12 mg of sodium borohydride solid in 5 ml dimethyl sulfoxide;

[0066] (2) mixed chloroauric acid solution and aminopyrimidine solution:

[0067] Mix the dimethyl sulfoxide ch...

Embodiment 2

[0075] Prepare a gold nanoparticle modified with 2-mercapto-4-aminopyrimidine, the steps are:

[0076] (1) Prepare reaction solution:

[0077] Preparation concentration is the chloroauric acid methanol solution of 5mM, is about to dissolve 41.2mg chloroauric acid solid in 20ml methanol and make;

[0078] Prepare a 2-mercapto-4-aminopyrimidine methanol solution with a concentration of 30 mM, that is, add 37.8 mg of 2-mercapto-4-aminopyrimidine solid to 10 ml of methanol, and add 300 μL of anhydrous acetic acid and 50 μl of Tween 20 to obtain a solution containing 2 - a mixed solution of mercapto-4-aminopyrimidine;

[0079] Prepare a sodium borohydride methanol solution with a concentration of 100mM, which is prepared by dissolving 20mg of sodium borohydride solid in 5ml of methanol;

[0080] (2) mixed chloroauric acid solution and aminopyrimidine solution:

[0081] Under ice-bath conditions, the chloroauric acid methanol solution prepared in step (1) was mixed with a mixed s...

Embodiment 3

[0089] Preparation of gold nanoparticles modified by 2,4-diamino-6-mercaptopyrimidine, the steps are:

[0090] (1) Prepare reaction solution:

[0091] Prepare the chloroauric acid tetrahydrofuran solution with a concentration of 5mM, which is obtained by dissolving 41.2mg of chloroauric acid solid in 20ml tetrahydrofuran;

[0092] Prepare a solution of 2,4-diamino-6-mercaptopyrimidine tetrahydrofuran with a concentration of 100 mM, that is, add 142 mg of 2,4-diamino-6-mercaptopyrimidine solid to 10 ml of tetrahydrofuran, and add 400 μl of anhydrous acetic acid and 80 μl of Tween 80, to obtain a mixed solution containing 2,4-diamino-6-mercaptopyrimidine;

[0093] Prepare a sodium ascorbate tetrahydrofuran solution with a concentration of 100 mM, which is obtained by dissolving 99 mg of sodium ascorbate solid in 5 ml tetrahydrofuran;

[0094] (2) mixed chloroauric acid solution and aminopyrimidine solution:

[0095] Mix the tetrahydrofuran solution of chloroauric acid prepare...

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Abstract

The invention provides series amiopyrimidine-modified gold nano particles. In the amiopyrimidine-modified gold nano particles, the amiopyrimidine may be one or more of 2-thiol-4,6-diamino-pyrimidine, 2-thiol-4-amino-pyrimidine and 2,4-diamino-6-mercaptopyrimidine. The particle size of the amiopyrimidine-modified gold nano particles is 1.5 to 10.0 nanometers; and the molar content ratio of amiopyrimidine molecules to gold element in the nano particles ranges from 0.1 to 0.9. The amiopyrimidine-modified gold nano particles have antibacterial effect, can be widely used for preparing antibacterial medicines such as anti-gram-negative bacilli penicillins medicines, anti-gram-positive bacterium medicines or / and anti-multi-medicine-resistance clinically isolated bacterium medicines. The invention also provides the preparation method and use of the amiopyrimidine-modified gold nano particles. The invention further provides antibacterial medicines containing the amiopyrimidine-modified gold nano particles.

Description

technical field [0001] The invention relates to a series of aminopyrimidine-modified gold nanoparticles, a preparation method of the nanoparticles and an antibacterial application thereof. Background technique [0002] At present, the research and development of nano-metal antibacterial drugs at home and abroad mainly focus on nano-silver, nano-titanium dioxide and nano-zinc oxide. But nano-silver is unstable to light and harmful to human body, the antibacterial effect of nano-titanium dioxide depends on the light source, and the dosage of nano-zinc oxide is too high. However, there are not many researches on gold nanoparticles as antibacterial agents. Sabo-Attwood T.L. et al. (Norman, R.S.; Stone, J.W.; Gole, A.; Murphy, C.J.; Sabo-Attwood, T.L., Targeted photothermallysis of thepathogenic bacteria, pseudomonas aeruginosa, with gold nanorods. Nano Lett. 2008, 8, ( 1), 302-306) use gold nanorods to absorb near-infrared light and generate heat to sterilize, but also require...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/505A61K33/24A61P31/04
CPCA61K31/505A61K9/16A61K47/02A61P31/00A61P31/04Y02A50/30
Inventor 蒋兴宇赵玉云田月刘文文张伟
Owner 重庆龙翼生物科技有限公司
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