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LAMP-oriented BAP31 vaccine vector capable of inducing immune response and application thereof

A vaccine carrier and immune response technology, applied in the field of DNA vaccines, can solve problems such as unseen expression, and achieve the effects of strong tumor cell growth, enhancement of cytotoxic activity, and inhibition of tumor cell growth

Active Publication Date: 2014-03-26
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The results of the study showed that BAP31 was expressed in thymus, cerebellum, pituitary gland, thyroid, kidney and ovarian tissues, but all of them were limited to a few cells, and no expression was seen in skeletal muscle

Method used

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  • LAMP-oriented BAP31 vaccine vector capable of inducing immune response and application thereof
  • LAMP-oriented BAP31 vaccine vector capable of inducing immune response and application thereof
  • LAMP-oriented BAP31 vaccine vector capable of inducing immune response and application thereof

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Embodiment Construction

[0034] In the present invention, the tumor-related gene BAP31 or the truncated gene ΔBAP31 corresponding to the dominant epitope is inserted into the LAMP sequence, and a recombinant vaccine vector containing BAP31 is constructed. After the vector is expressed, it shows a specific immune induction effect and has anti-tumor effect. characteristic. In the following, the present invention will be further described in detail in conjunction with the construction of the recombinant vaccine vector of BAP31, the immunization of DNA vaccine and the detection of its immune effect and anti-tumor effect, which is an explanation of the present invention rather than a limitation.

[0035] The following details the construction process of cloning human BAP31 or ΔBAP31 (truncated gene) vaccine vector:

[0036] 1. Cloning of full-length and truncated genes of human BAP31

[0037] Digest Collection 5×10 6 Add 1ml Trizol solution to Hela cells and incubate at room temperature for 5min to extra...

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Abstract

The invention discloses a lysosome-associated membrane protein (LAMP)-oriented B cell receptor associated protein 31 (BAP31) vaccine vector capable of inducing immune response and application thereof. In the vaccine vector, an associated gene BAP31 of a tumor or a truncated gene delta BAP31 corresponding to a dominant epitope is inserted between a lumen structural domain and transmembrane / cytoplasmid tail of an LAMP sequence to construct a recombinant vaccine vector containing BAP31. The vector is expressed to form fusion protein, so that the BAP31 positioned between an LAMP molecular lumen and a transmembrane / cytoplasmid tail is oriented to lysosome to perform the processing and assembly of a major histocompatibility complex-II (MHCII) / antigen peptide complex. Therefore mice are immunized by the recombinant vaccine vector, so that antitumor specific humoral immune response and cell immune response can be induced efficiently and melanoma cells B16 of the mice with high-expression BAP31 can be killed specifically.

Description

technical field [0001] The invention belongs to the technical field of DNA vaccines, and relates to a BAP31 vaccine carrier capable of inducing immune response and having LAMP orientation and application thereof. Background technique [0002] Malignant tumors are currently one of the major diseases that seriously affect human health and threaten human life. Therefore, the World Health Organization and government health departments of various countries have listed cancer as a top priority. At the 18th International Anti-Cancer Union Conference, a research report published by the World Health Organization showed that the global cancer situation will become increasingly serious, and the number of new patients will increase from the current 10 million to 15 million per year in the next 20 years. The number of people will also increase from 6 million to 10 million per year. In response to the above severe situation, the report calls on all countries to formulate national anti-ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00
Inventor 杨琨宋朝君金伯泉李海涛孙元杰魏玉英
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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