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Carrier transferred adjuvant antigen and its preparing method

A technology of antigen and adjuvant, which is applied in the field of vaccine adjuvant carrier system and its preparation, and carrier delivery of adjuvanted antigen, which can solve the problems of liposome structure rupture, uneven raw materials, high cost, etc., and achieve the goal of reducing side effects Effect

Inactive Publication Date: 2004-12-15
SHANGHAI INST OF BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main raw material in the phospholipid liposome is lecithin, the raw material is not uniform, contains more unsaturated bonds, and it is prone to peroxidation reaction, forming toxic peroxides and causing the liposome structure to rupture. to improve, but expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Liposome-delivered Tetanus Toxoid (TT) and Antibody Response Induced by PE-CpG

[0017] Balb / c mice were immunized with non-phospholipid liposomes (Np) wrapped with TT and PE-CpG, and immunized three times at 0, 3, and 6 weeks. Blood was collected one week after the last immunization, and the antibody titer was determined by indirect ELISA method. Compared with the TT group of adjuvant, aluminum adjuvant and CFA, the antibody titer induced was about 20 times that of the no adjuvant and aluminum adjuvant group, and 3 times that of the CFA group. The results of antibody subclass analysis showed that liposome-delivered TT and PE-CpG induced high levels of IgG2a and IgG1, and induced a balanced immune response of Th1 / Th2 type.

[0018] Table 1: Specific antibody and subclass titers induced by different adjuvant TT groups

[0019] Group IgG2a IgG1 IgG2a / IgG1 Total IgG

[0020] None <100 16117 0 45600

[0021] Al 672 61466 0.1 42380

[0022] CFA 30300 167565 0.2...

Embodiment 2

[0024] Example 2 Antibody response induced by liposome delivery of inactivated JEV vaccine (inactivate JEV) and PE-CpG

[0025] Balb / c mice were immunized with non-phospholipid liposome (Lp) encapsulated JE inactivated vaccine and PE-CpG, with no adjuvant and CFA group as the control, immunized twice at 0, 3, weeks, and collected at the 9th week The serum was diluted 50 times, and the live JE virus was used to coat the plate, and the anti-JEV antibody was detected by the indirect ELISA method, and the result was expressed in OD490nm. Immunization with PE-CpG and inactivated JE vaccine after Lp wrapping can increase the specific antibody titer induced by JE inactivated vaccine.

[0026] Table 2: Anti-JEV specific antibody (OD490nm) of immunized mice in the ninth week

[0027] Vaccine Adjuvant Total IgG

[0028] JE inactivated vaccine None 0.174±0.042

[0029] JE inactivated vaccine CFA 0.617±0.012 ab

[0030] JE inactivated vaccine LpCpG 0.906±0.011 ab

[0031] Normal mice...

Embodiment 3

[0033] Example 3 Cellular immunity induced by liposome-delivered Japanese encephalitis inactivated vaccine (inactivate JEV) and PE-CpG

[0034] Balb / c mice were immunized with non-phospholipid liposome (Lp) encapsulated JE inactivated vaccine and PE-CpG, with no adjuvant and CFA group as control, immunized twice at week 0, 3, and at week 5 Spleen lymphocytes from immunized mice were stimulated with virus in vitro and cultured to collect mother cells. The primary kidney cells of Balb / c suckling mice infected with Japanese encephalitis virus for 12 hours were used as target cells, and the effect-to-target ratio was 20:1 by MTT method. Detection of CTLs. The results showed that the inactivated JE vaccine only induced very low CTL activity, and the PE-CpG delivered by Lp and the CTL activity induced by the inactivated JE vaccine had a significant increase, which was similar to that induced by the live attenuated JE vaccine. CTL activity was comparable.

[0035] Table 3: Anti-JEV...

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Abstract

The present invention belongs to the field of immunological preparation in biotechnology, and relates to adjuvant carrier system of vaccine and its preparation process and application. The present invention prepares antigen into adjuvant and coats the antigen adjuvant with liposome carrier to prepare antigen adjuvant carrier system. The adjuvant and the antigen are transferred simultaneously via the carrier. Experiment shows that after being diluted and administrated, powerful body fluid and cell immune response may be induced to raise the absorption of vaccine and lower toxic side effect. The present invention may be used in preventive and treating vaccine, especially treating vaccine for lasting viral infection.

Description

technical field [0001] The invention belongs to the field of biotechnology immune preparations, and relates to a vaccine adjuvant carrier system and a preparation method and application thereof. Specifically, the present invention uses a carrier to deliver an adjuvanted antigen, which can induce strong humoral and cellular immune responses after dilution and administration, and can be used for the prevention and treatment of infectious diseases. Background technique [0002] Existing studies have shown that the cause of persistent viral infection such as chronic hepatitis B virus persistent infection is due to the body's defective immune response. How to effectively activate the body's response to the persistent virus infection and generate the humoral and cellular immune responses needed to clear the virus, especially the cellular immune response, is currently a hot spot in the development of therapeutic vaccines. [0003] According to reports, the immunogenicity of protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K47/10A61K47/28A61P31/12A61P37/02
Inventor 何春艳刘庆良
Owner SHANGHAI INST OF BIOLOGICAL PROD CO LTD
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