Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid

A technology of aflatoxin and linolenic acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the mechanism of action of Oxylipins products is not very clear.

Inactive Publication Date: 2011-10-12
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the mechanism of action of these Oxylipins products is not very clear

Method used

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  • Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid
  • Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid
  • Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid

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Embodiment 1

[0028] Example 1, α-linolenic acid inhibits aflatoxin production

[0029] Aspergillus flavus (CGMCC NO.3.2890) was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences; the initiation of culture of the strains: A small amount of the strains frozen in glycerol at -80°C was evenly spread on the PDA medium for activation. After culturing at 37°C for 4 days under aerobic conditions, green spores can be observed.

[0030] The preparation method of the PDA medium is as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil until it can be punctured by a glass rod for 30 minutes, filter with four layers of gauze, and then add 20g of glucose and 15g of agar, continue to heat and stir to mix, then make up water to 1000 ml after cooling down, seal with air-permeable film, take it out after high pressure (121°C) sterilization for 15 minutes, and pack it into disposable plastic petri dishes (9 cm in d...

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Abstract

The invention discloses a method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid. The invention provides an application of alpha-linolenic acid and / or analogues thereof and / or derivatives thereof to inhibiting the generation of the aflatoxin. The invention also provides an application of the alpha-linolenic acid and / or analogues thereof and / or derivatives thereof to preparing a product for inhibiting the generation of the aflatoxin. In the invention, experiments show that the alpha-linolenic acid can inhibit the generation of the aflatoxin and avoid the damage of the aflatoxin on a human body; in the research, by using a metabonomic means, the relation between the generation of toxin and fatty acid is known from a metabolic level and a metabolic way related to toxin synthesis is found through comparing panoramic metabolites changes of generating the toxin or not, caused by treating through the linolenic acid or not treating; and the synthesis of the toxin is reduced through blocking a synthesis way.

Description

technical field [0001] The present invention relates to a method for inhibiting aflatoxin production by using α-linolenic acid. Background technique [0002] Linolenic acid: English name, linolenic acid, is a polyunsaturated fatty acid containing three double bonds. Due to the position of the double bond, it is divided into α-linolenic acid and γ-linolenic acid. [0003] α-linolenic acid, the chemical formula is: cis-9-cis-12-cis-15-octadecatrienoic acid (C 18 h 30 o 2 ), molecular weight: 278.43. CAS: 463-40-1; Structural formula: [0004] [0005] α-linolenic acid is one of the essential fatty acids in the human body, and it is also a precursor molecule that produces signal molecules when plants are under stress. [0006] Aflatoxins are among the most toxic and carcinogenic compounds found in nature. Aflatoxin contamination in agricultural products seriously threatens food safety and causes huge economic losses. Nearly half a century of research on Aspergillus f...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/67
Inventor刘春明晏石娟张晋丹
OwnerINST OF BOTANY CHINESE ACAD OF SCI