Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid
A technology of aflatoxin and linolenic acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that the mechanism of action of Oxylipins products is not very clear.
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[0028] Example 1, α-linolenic acid inhibits aflatoxin production
[0029] Aspergillus flavus (CGMCC NO.3.2890) was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences; the initiation of culture of the strains: A small amount of the strains frozen in glycerol at -80°C was evenly spread on the PDA medium for activation. After culturing at 37°C for 4 days under aerobic conditions, green spores can be observed.
[0030] The preparation method of the PDA medium is as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil until it can be punctured by a glass rod for 30 minutes, filter with four layers of gauze, and then add 20g of glucose and 15g of agar, continue to heat and stir to mix, then make up water to 1000 ml after cooling down, seal with air-permeable film, take it out after high pressure (121°C) sterilization for 15 minutes, and pack it into disposable plastic petri dishes (9 cm in d...
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