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50 results about "Toxin synthesis" patented technology
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Here, we report that C. difficile regulates its toxin synthesis by quorum signaling, in which a novel signaling peptide activates transcription of the disease-causing toxin genes. This finding provides new therapeutic targets to be harnessed for novel nonantibiotic therapy for C. difficile infections.
Multi-functional Streptomyces amritsarensis and application thereof
The invention relates to the field of micro-organisms, and particularly relates to a multifunctional Streptomyces amritsarensis and application thereof. Streptomyces amritsarensis HG-16, with a preservation number of CCTCC-M-2018237. The streptomycin cannot only kill the microcystin, but also antagonize the fusarium graminearum. The fermentation liquid and the active secretion can effectively inhibit the production of the microcystin in a short time, can inhibit the expression of the synthetic gene of 90% microcystin within 9 hours, and can completely inhibit the expression of the microcystingene after 36 hours. Three days after the addition of the Streptomyces, the inhibition rate of the algae toxin is 93%; while for the algicidal bacteria which has been reported to have the effect of inhibiting the production of the microcystin needs more than 3 days to inhibit the synthesis of the microcystin.
Owner:SOUTHWEST UNIV
Deep-sea Bacillus aryabhattai capable of inhibiting aflatoxin
InactiveCN105039197APrevention and Control of PollutionBacteriaMicroorganism based processesToxin synthesisBacillus aryabhattai
The invention provides a deep-sea bacterium capable of inhibiting synthesis of aflatoxin, in particular to Bacillus aryabhattai ZL4, isolated from light yellow calcareous ooze sediment 2796 meters deep in South Atlantic and preserved in China General Microbiological Culture Collection Center under CGMCC No. 10661. The Bacillus aryabhattai ZL4 is fermented in a screened culture medium, acquired fermented supernate is fully capable of inhibiting synthesis of aflatoxin, no toxic intermediates are generated, and the Bacillus aryabhattai ZL4 is able to inhibit the aflatoxin from the toxin synthetic means but has no inhibitory action on intestinal flora and unicellular eumycetes, saccharomyces cerevisiae. The Bacillus aryabhattai ZL4 and its fermentation products are applicable to effective control of aflatoxin contamination of fodders, grains, field crops and the like.
Owner:HARBIN INST OF TECH AT WEIHAI +1
Kit for synchronously detecting six fungaltoxin producing fungi
ActiveCN108315460AEasy to pre-interventionHarm reductionMicrobiological testing/measurementMicroorganism based processesToxin synthesisMultiplex pcrs
The invention discloses a kit for synchronously detecting six fungal toxins. The six toxins are aflatoxin, ochratoxin, patulin, trichothecene mycotoxin, fumonisins and zearalenone; and based on a multiple PCR detection method, the kit comprises a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair and an internal standard primer pair which can perform PCR reaction in the same reaction tube. The detection system directly targets a toxin synthesis essential gene and has high specificity; an internal reference gene is amplified synchronously, positive, negative and blank control are set simultaneously, and false negative and false positive are effectively avoided; and multiplication of PCR index guarantees the sensitivity of the method.
Owner:厦门市产品质量监督检验院
Method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid
InactiveCN102212481AInhibitionReduce synthesisFungiMicroorganism based processesMetaboliteToxin synthesis
The invention discloses a method for inhibiting generation of aflatoxin by utilizing alpha-linolenic acid. The invention provides an application of alpha-linolenic acid and / or analogues thereof and / or derivatives thereof to inhibiting the generation of the aflatoxin. The invention also provides an application of the alpha-linolenic acid and / or analogues thereof and / or derivatives thereof to preparing a product for inhibiting the generation of the aflatoxin. In the invention, experiments show that the alpha-linolenic acid can inhibit the generation of the aflatoxin and avoid the damage of the aflatoxin on a human body; in the research, by using a metabonomic means, the relation between the generation of toxin and fatty acid is known from a metabolic level and a metabolic way related to toxin synthesis is found through comparing panoramic metabolites changes of generating the toxin or not, caused by treating through the linolenic acid or not treating; and the synthesis of the toxin is reduced through blocking a synthesis way.
Owner:INST OF BOTANY CHINESE ACAD OF SCI
Synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone
PendingCN108342500AEasy to pre-interventionHarm reductionMicrobiological testing/measurementDNA/RNA fragmentationPositive controlToxin synthesis
The invention discloses a synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone. The synchronous detection kit of fungi capable of producing trichothecene mycotoxins, fumonisins, and zearalenone is invented based multiple PCR detection, and comprises a first primer pair, a second primer pair, a third primer pair, and an internal standard primer pair which are capable of realizing PCR reaction in a same reaction tube. A detection system disclosed in the invention is capable of realizing direct targeting at toxin synthesis essential genes,and is high in specificity; synchronous amplification of reference genes is realized, a positive control, a negative control, and a blank control are provided at the same time, so that false negativeand false positive are avoided effectively; and PCR index multiplication is capable of ensuring method sensitivity.
Owner:厦门市产品质量监督检验院(国家半导体发光器件应用产品质量监督检验中心、国家场(厂)内机动车辆质量监督检验中心)
Biosynthesis method of T-2 toxin
ActiveCN103421856AEasy to handleEasy to operateOrganic chemistryMicroorganism based processesToxin synthesisFusarium
The invention belongs to the technical field of veterinary pharmacology and relates to a biosynthesis method of a T-2 toxin. The biosynthesis method utilizes corn particles as raw materials and comprises the following steps of carrying out fusarium graminearum sherbakoff recovery, carrying out conidium production, inoculating the corn toxin-production medium with the conidia, carrying out culture by the corn toxin-production medium, and carrying out ethanol extraction, dichloromethane extraction, one-step column chromatography and crystallization on the medium in a toxin-production peak stage to obtain T-2 toxin crystals. The biosynthesis method has extraction efficiency of 89%. Nuclear magnetism and mass spectrum structure identification proves that the T-2 toxin is correct. The T-2 toxin has the content greater than or equal to 95.66% and has a stable period more than 4 months. Compared with the traditional extraction technology, the biosynthesis method has the advantages that the one-step column chromatography can separate and purify a large amount of the T-2 toxin; the processes are simple and fast; a yield is high; the T-2 toxin has stable properties and high purity; a T-2 toxin synthesis cost is greatly reduced; the T-2 toxin synthesis processes are simplified; and the T-2 toxin can be widely used in toxicology and metabolism test research fields related to various toxins.
Owner:HUAZHONG AGRI UNIV
Application of antibacterial lipopeptide Fengycin in inhibition of aspergillus flavus growth and toxin synthesis
ActiveCN112661807AGood inhibitory effectEnhanced inhibitory effectPlant growth regulatorsBiocideBiotechnologySporeling
The invention provides an application of an antibacterial lipopeptide Fengycin in inhibition of aspergillus flavus growth and toxin synthesis, and belongs to the technical field of bioengineering. The antibacterial lipopeptide Fengycin provided by the invention is a mixture of antibacterial lipopeptide Fengycin A and antibacterial lipopeptide Fengycin B. The antibacterial lipopeptide Fengycin is derived from a fermentation extract of Bacillus megaterium, and tests prove that the antibacterial lipopeptide Fengycin has obvious inhibition effects on aspergillus flavus filament extension, spore germination and aflatoxin biosynthesis. Besides, the fermentation medium of the marine bacillus megaterium is optimized, the content of the antibacterial lipopeptide generated by the optimized fermentation medium is (571.26 + / -9.72) mg / L, and a foundation is laid for 1.82 times of the yield before optimization and large-scale production of the antibacterial lipopeptide for inhibiting aspergillus flavus.
Owner:OCEAN UNIV OF CHINA
Method for defining stages of development of cyanobacterial bloom
PendingUS20210348211A1Microbiological testing/measurementTesting plants/treesToxin synthesisPhycocyanin
Disclosed is a method for defining the stages of development of a cyanobacterial bloom, wherein the development of a cyanobacterial bloom is divided into five stages: a cyanobacteria wintering period, a resuscitation period, a rapid growth period, an outbreak period and a cyanobacteria decline period; the wintering period is defined based on the concentration / content ratio of algocyan / chlorophyll a; the resuscitation period is defined based on the relative expression levels of the phycocyanin synthesis gene PC-IGS, the algal toxin synthesis gene mcyA and the gas vesicle synthesis gene gvpC in the surface sediment; the cyanobacteria rapid growth period is defined based on the relative expression level of the ftsZ gene; the cyanobacteria outbreak period is defined based on the wind speed; and the cyanobacteria decline period is defined based on the relative expression level of the nblA gene and the ratio of glucose to neutral polysaccharides in dissolved organic matter.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY
Quick molecular detection method for simultaneously detecting three kinds of fusarium toxins and application thereof
InactiveCN102094080BSimple, fast and reliable identificationToxin detectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyToxin synthesis
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Aflatoxin inhibitor
The invention relates to a food additive, in particular to an aflatoxin inhibitor in which 1.2%o of mannan oligosaccharide is added. The aflatoxin inhibitor can inhibit growth of toxin-producing aspergillus flavus and reduce toxin synthesis of the toxin-producing aspergillus flavus; in addition, mannan oligosaccharide is a green additive, secondary contamination to food cannot be caused, the problem that thick broad-bean sauce cannot be eaten due to the fact that aflatoxin in the thick broad-bean sauce exceeds the standard is solved, the quality safety of the thick broad-bean sauce is guaranteed, and a wider application prospect is created for the thick broad-bean sauce.
Owner:CHENGDU NORMAL UNIV
Preparation method, intermediates, and use of cyclic peptide toxin alpha-amanitin and / or amaninamide
ActiveUS20250282826A1Peptide preparation methodsBulk chemical productionCyclic peptideOrganic synthesis
A preparation method, intermediates, and use of a cyclic peptide toxin α-Amanitin and / or Amaninamide are provided, belonging to the technical field of organic synthesis. A total synthesis method of the cyclic peptide toxin compounds α-Amanitin and Amaninamide is provided, where raw materials and reagents used are easily purchased through commercial channels, the intermediates are stable, and the preparation method shows mild reaction conditions, simple operation process, desirable operability of separation and purification, and high yield. The preparation method also shows important reference and practical value, can achieve gram-scale preparation of the α-Amanitin and Amaninamide, and has excellent industrial prospects. Therefore, the preparation method is of significant application value in the field of cyclic peptide toxin synthesis.
Owner:INNER MONGOLIA UNIVERSITY +1
Synchronous test kit of fungi to produce aflatoxin, ochratoxin and patulin
ActiveCN108148921AEasy to pre-interventionHarm reductionMicrobiological testing/measurementDNA/RNA fragmentationInternal standardToxin synthesis
The invention discloses a synchronous test kit of fungi to produce aflatoxin, ochratoxin and patulin, which, based on a multiple PCR (polymerase chain reaction) detection method, includes a first primer pair, a second primer pair, a third primer pair and an internal standard primer pair which may carry out PCR in a same reaction tube. A test system herein directly targets at genes necessary to toxin synthesis and has high specificity; internal reference genes are amplified synchronously; positive, negative and blank controls are provided; false negative and false positive are effectively avoided; the PCR index doubling ensures method sensitivity.
Owner:厦门市产品质量监督检验院(国家半导体发光器件应用产品质量监督检验中心、国家场(厂)内机动车辆质量监督检验中心)
Application of marine phycobacterium LZ-14T in production of indole-3-acetic acid
The invention belongs to the technical field of microorganisms, and particularly relates to application of marine phycobacterium LZ-14T in production of indole-3-acetic acid. According to the present invention, the interphycobacterium LZ-14T is named as Maricatenella alexandrii gen. Nov., sp. Nov. The present invention discovers that the interphycobacterium LZ-14T can achieve the effect of significantly inhibiting the synthesis of the PSP toxin by releasing the IAA signal molecule under the condition of promoting the growth of the host algae. The new discovery can provide a new theoretical basis for analyzing the generation mechanism of paralytic shellfish poison of alexandrium chainchain and exploring scientific prevention and control of red tide. The invention also finds that the IAA produced by the phycobacterium LZ-14T can inhibit the growth of escherichia coli, and the result lays a foundation for the preparation of new antibacterial drugs.
Owner:ZHEJIANG OCEAN UNIV +1
Method for researching influence on generation of microcystic toxin by chloride ions and sulfate radicals by using algae toxin toxin-producing gene
InactiveCN107604051AStrong specificityHigh sensitivityMicrobiological testing/measurementBiostatisticsMicrocystinSulfate radicals
The invention relates to a method for researching influence on generation of microcystic toxin by chloride ions and sulfate radicals by using an algae toxin toxin-producing gene. The chloride ions andsulfate radicals with different concentrations are added in a nutrient solution, the microcystic toxin content and a copy number of the algae toxin toxin-producing gene mcyD are detected, correlationamong the chloride ions and sulfate radicals with different concentrations, the microcystic toxin content and a copy number of the algae toxin toxin-producing gene mcyD is established, and the content of the algae toxin is researched by detecting the copy number of the algae toxin synthetase gene mcyD. The method has the advantages of strong specificity, high sensitivity, short required time, simple operation, and simple required equipment and reagent.
Owner:SHANGHAI MUNICIPAL WATER RESOURCES DEV & UTILIZATION NAT ENG CENT +1
Method for researching influence on hemolytic activity of marine microalgae based on transcriptome technology
PendingCN121249843AMicrobiological testing/measurementData visualisationBiotechnologyToxin synthesis
The invention discloses a method for researching influence on hemolytic activity of marine microalgae based on a transcriptome technology, and relates to the field of hemolytic activity analys.The method comprises the steps that pretreatment is conducted on the marine microalgae based on culture requirements, and cystic morphological cell density and hemolytic activity of the marine microalgae are measured according to experimental design rules after pretreatment is completed; carrying out transcriptome sample collection operation by utilizing the marine microalgae subjected to pretreatment, constructing a library according to a transcriptome sample collection result, and obtaining a gene function annotation and a differential expression gene result; the growth conditions of the marine microalgae under different temperature conditions are analyzed according to cystic morphological cell density and hemolytic activity, and the regulatory gene influencing the hemolytic activity of the marine microalgae is obtained by combining gene function annotation and differential expression gene results. According to the method, the hemolytic activity of the marine microalgae cultured under different temperature conditions is extracted, so that the aim of researching related metabolic pathways possibly participating in toxin synthesis and hemolytic activity regulation is fulfilled.
Owner:GUANGXI ACAD OF SCI
A simultaneous detection kit for six types of mycotoxin-producing bacteria
ActiveCN108315460BEasy to pre-interventionHarm reductionMicrobiological testing/measurementMicroorganism based processesMultiplexReference genes
The invention discloses a simultaneous detection kit for fungi producing six toxins, the six toxins being aflatoxin, ochratoxin, patulin, trichothecene toxins, fumonisins and zearalen Ketone, based on the multiplex PCR detection method, including the first primer pair, the second primer pair, the third primer pair, the fourth primer pair, the fifth primer pair, the sixth primer pair and the inner indexer pair. The detection system of the present invention directly targets genes necessary for toxin synthesis, and has strong specificity; internal reference genes are synchronously amplified, and positive, negative and blank controls are set at the same time, effectively avoiding false negatives and false positives; PCR index multiplication ensures the sensitivity of the method.
Owner:厦门市产品质量监督检验院
Sinkiang sinorhizobium sp. APXJHT24-1 with functions of promoting growth and inhibiting aspergillus flavus and application of Sinkiang sinorhizobium sp. APXJHT24-1
The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Streptomyces amritsarensis and its application
The invention relates to the field of microorganisms, in particular to multifunctional Streptomyces amritsarensis alginolytic streptomyces and applications thereof. Streotomyces amritsarensis HG‑16, the preservation number of which is CCTCC M 2018237. The alginolytic streptomyces can not only kill Microcystis, but also antagonize Fusarium graminearum. Streptomyces alginolyticus, its fermentation broth and active secretions can effectively inhibit the production of microcystins in a short period of time. Adding the Streptomyces can inhibit the expression of 90% of the synthetic genes of microcystins within 9 hours, and within 36 hours After that, the expression of microcystin gene can be completely inhibited. After adding the Streptomyces for 3 days, the synthesis inhibition rate of algae toxin is 93%, while the alginolytic bacteria that have been reported to inhibit the synthesis of microcystin need more than 3 days to be able to inhibit production of microcystins.
Owner:SOUTHWEST UNIV
Application of plant secondary metabolite in inhibiting pathogenicity of fusarium oxysporum
The invention relates to an application of a plant secondary metabolite in inhibiting pathogenicity of fusarium oxysporum. The application comprises the step of realizing an antibacterial effect through at least one mechanism of inhibiting hypha growth, spore germination, cell membrane permeability change, defensive enzyme activity reduction, cell wall degrading enzyme activity inhibition or toxin synthesis reduction of pathogenic bacteria. The antibacterial action mode of the 2, 4-di-tert-butylphenol is that the 2, 4-di-tert-butylphenol can destroy the completeness of cell membranes of fusarium oxysporum of cucumbers and disturb the functions of the cell membranes to cause leakage of cell contents and oxidative damage, so that normal physiological activities of cells are inhibited. Meanwhile, 2, 4-di-tert-butylphenol can inhibit defensive enzyme activity and cell wall degrading enzyme activity of fusarium oxysporum and has a remarkable inhibition effect on toxin synthesis, so that the purpose of inhibiting fusarium oxysporum hyphae from infecting host plants is achieved, and a basis is provided for subsequent deeper research on a fusarium oxysporum inhibition mechanism.
Owner:YANGZHOU UNIV
Strain of sinorhizobium meliloti ASZJHZ25-4 with functions of promoting growth and inhibiting aspergillus flavus and application of sinorhizobium meliloti ASZJHZ25-4
The invention discloses sinorhizobium meliloti ASZJHZ25-4 with functions of efficiently promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium meliloti ASZJHZ25-4, and the preservation number of the sinorhizobium meliloti ASZJHZ25-4 is CCTCC M 2026230. According to the invention, the integration of two functions of generating a characteristic antibacterial volatile matter of 2-methylbutyric acid and efficiently symbiotic nitrogen fixation on a single strain is realized in the sinorhizobium meliloti for the first time. The 2-methylbutyric acid volatile matter released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously achieved at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer is formed in a soil microenvironment in a legume development critical period.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Bacteriostatic agent and application thereof in control of postharvest diseases of fruits and vegetables and synthesis of patulin
The invention provides an effective and safe green bactericide and application thereof. The green bactericide is a composition containing methyl furoate. Mycelium growth of penicillium expansum is remarkably inhibited through methyl furoate treatment, and toxin synthesis can be completely inhibited under the median lethal concentration. Therefore, the methyl furoate can be used for preparing a novel antifungal preservative, and is applied to the field of prevention and control of fruit and vegetable postharvest fungal diseases and toxin synthesis.
Owner:INST OF BOTANY CHINESE ACAD OF SCI
Sinorhizobium freudensis ASHBWH-23-1 with functions of promoting growth and inhibiting aspergillus flavus and application of sinorhizobium freudensis ASHBWH-23-1
The invention discloses a sinorhizobium fredii strain capable of promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium fredii strain. The sinorhizobium fredii strain is classified and named as sinorhizobium fredii, and the preservation number of the sinorhizobium fredii strain is CCTCC (China Center for Type Culture Collection) M 20253054. According to the invention, the dual functions of generating a specific bacteriostatic volatile matter 2-nonanol and efficiently symbiotic nitrogen fixation are integrated in the sinorhizobium freudenreanum for the first time. 2-nonanol released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Deep-sea actinomycete rhodococcus erythropolis and application thereof in inhibition of aflatoxin
The invention provides a deep-sea actinomycete strain capable of inhibiting synthesis of aflatoxin. The actinomycete strain is a rhodococcus erythropolis BC14-M2AF-1 strain, is separated from sediments at water depth of 6105 meters in West Pacific Ocean, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No.61239. The BC14-M2AF-1 strain is fermented in a freshwater M2 culture medium, and the obtained fermented cell-free supernate can completely inhibit aflatoxin from the source of an aflatoxin synthesis route; and the fermentation cell-free supernate is treated at 121 DEG C and 103 kPa for 30 minutes at high temperature and high pressure, and synthesis of aflatoxin can still be inhibited by 100%. The rhodococcus erythropolis BC14-M2AF-1 strain and the fermentation product thereof can be used for effectively preventing and controlling aflatoxin pollution in field crop cultivation, grain storage, food and beverage processing, feed production and the like.
Owner:HARBIN INST OF TECH AT WEIHAI +1
Gene FgPHM1 for regulating synthesis and pathogenicity of fusarium graminearum toxin DON and application thereof
The invention discloses a gene FgPHM1 for regulating synthesis and pathogenicity of fusarium graminearum toxin DON and application of the gene FgPHM1, and belongs to the field of plant disease prevention and control. A novel gene FgPHM1 for regulating and controlling synthesis and pathogenicity of toxin DON is found in plant pathogenic fungus fusarium graminearum, and the nucleotide sequence of the gene FgPHM1 is as shown in SEQ ID NO. 1. Compared with a wild strain PH-1, the FgPHM1 gene knockout mutant has the advantages that the colony diameter of the mutant is reduced, the synthesis of the toxin DON is remarkably reduced, and the pathogenicity to wheat ears is lost, so that the FgPHM1 gene plays an important role in regulating and controlling the synthesis and pathopoiesis processes of the fusarium graminearum toxin DON. The invention provides important resources and research directions for prevention and control of pollution of toxin DON in agricultural products and prevention and control of wheat scab by using the FgPHM1 gene.
Owner:MINJIANG UNIVERSITY
Rhizobium albiziae APHNZY-24-4 with functions of promoting growth and inhibiting aspergillus flavus and application of rhizobium albiziae APHNZY-24-4
The invention discloses a rhizobium mesonii strain APHNZY-24-4 which has the functions of promoting peanut growth and inhibiting aspergillus flavus, and the preservation number of the rhizobium mesonii strain APHNZY-24-4 is CCTCC (China Center for Type Culture Collection) M 2026228. According to the invention, integration of two functions of generating a specific bacteriostatic volatile matter, namely 3-methylbutyric acid, and efficient symbiotic nitrogen fixation on a single strain is realized in the rhizobium japonicum for the first time. 3-methylbutyric acid released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Application of N-(phenylthio) phthalimide in reducing toxin content of wheat
ActiveCN111727973AInhibition relative expressionEffective controlBiocideFungicidesChemical compoundToxin synthesis
The invention discloses application of N-(phenylthio) phthalimide in reducing the toxin content of wheat. The application is to inhibit the synthesis of wheat B-type trichothecene toxins, reduce the content of the wheat B-type trichothecene toxins or inhibit Tri5 gene expression in fusarium graminearum. The compound N-(phenylthio) phthalimide is a compound shown as a formula (I). The invention hasimportant value for controlling wheat scab toxin pollution.
Owner:ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
A kind of synthetic method of trichothecenes B group toxin
ActiveCN103421857BEasy to handleEasy to operateOrganic chemistryMicroorganism based processesSynthesis methodsToxin synthesis
The invention belongs to the technical field of biotoxin synthesis, relates to the technical field of biological fermentation and discloses a synthesis method of type-B trichothecene toxins. The synthesis method utilizes rice particles as raw materials and comprises the following steps of reviving fusarium after inoculation, producing conidium, inoculating a rice toxin-production medium with the conidium, carrying out methanol liquid extraction, saturation, extraction, column chromatography, hydrolysis and crystallization of the medium so that type-B trichothecene toxin 3Ac-DON and toxin DON crystals are obtained. After primary crystallization, the toxin crystals are subjected to nuclear magnetism and mass spectrum structure identification and a result shows that the toxin crystals are correct and have the content greater than or equal to 94%. Compared with the traditional synthesis method, the synthesis method provided by the invention utilizes a one-step column chromatography technology to realize separation and purification of the toxin 3Ac-DON, has simple and fast processes and a high yield, produces the toxin having stable properties and high purity, obviously reduces a toxin synthesis cost, realizes production of the toxin DON from the toxin 3Ac-DON only through one-step hydrolysis, avoids pigment interference, is simple and saves time and labor. The two toxins obtained by the synthesis method can be widely used in field of toxins used in toxicology and metabolism experiments.
Owner:HUAZHONG AGRI UNIV
Bradyrhizobium anhuiensis ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application of bradyrhizobium anhuiensis ASHBWH-23-9
The invention discloses bradyrhizobium yuanmingense ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application of the bradyrhizobium yuanmingense ASHBWH-23-9, and the preservation number of the bradyrhizobium yuanmingense ASHBWH-23-9 is CCTCC (China Center for Type Culture Collection) M 20253053. The innovation of the invention lies in that the dual functions of generating cyclopentanone antibacterial volatile matters and efficiently symbiotically fixing nitrogen are integrated in the bradyrhizobium in the circular and clear garden for the first time. The molecular mechanism is that the generated volatile substance can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Compared with an existing microbial agent with a single function, nodulation and nitrogen fixation promotion and aspergillus flavus inhibition at the source can be synchronously realized at the rhizosphere of the peanuts through one-time inoculation, and particularly, aiming at the characteristics of overground flowering and underground fruiting of the peanuts, the microbial agent continuously plays a role in bacteriostasis and antipoison in soil in the pod development period.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
Application of prothioconazole/carbendazim compound bactericide in controlling gibberellic disease
InactiveCN104604906ALower doseImprove the effect of prevention and controlBiocideFungicidesToxin synthesisPlant disease
The invention discloses an application of a prothioconazole / carbendazim compound bactericide in controlling a gibberellic disease. A mass ratio of prothioconazole to carbendazim in the compound bactericide is 1:(0.1-5). According to the application, two bactericides with different mechanisms of action are mixed for use; on the basis that a good effect of carbendazim on the gibberellic disease of wheat crops is kept, carbendazim is compounded with a novel demethylation inhibitor, namely prothioconazole, so that the dosage of the bactericide is reduced; a control effect is improved; and the compound bactericide has a significant synergistic action on the disease of the wheat crops, can greatly inhibit toxin synthesis of pathogenic bacteria, improves grain quality, solves the problem of resistance caused by single use of carbendazim at the same time, is efficient, and low in toxicity and residue, reduces the dosage of a single bactericide and saves cost.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Application of antibacterial lipopeptide fengycin in inhibiting the growth and toxin synthesis of Aspergillus flavus
The invention proposes the application of an antibacterial lipopeptide Fengycin in inhibiting the growth of Aspergillus flavus and the synthesis of toxin, which belongs to the technical field of bioengineering. The antibacterial lipopeptide Fengycin provided by the present invention is a mixture of antibacterial lipopeptide Fengycin A and antibacterial lipopeptide Fengycin B. The antibacterial lipopeptide Fengycin is derived from the fermentation extract of marine Bacillus megaterium. It has been proved by experiments that the antibacterial lipopeptide Fengycin It has obvious inhibitory effect on Aspergillus flavus hyphae elongation, spore germination and aflatoxin biosynthesis. In addition, the present invention also optimizes the fermentation medium of Bacillus megaterium, and the antimicrobial lipopeptide content produced by the optimized fermentation medium is (571.26±9.72) mg / L, which is 1.82 times of the output before optimization , laying the foundation for the large-scale production of antibacterial lipopeptides that inhibit Aspergillus flavus.
Owner:OCEAN UNIV OF CHINA