Multi-functional Streptomyces amritsarensis and application thereof

A multifunctional technology of Streptomyces alginolyticus, applied in the field of microorganisms, can solve the problems of pathogenic bacteria production, reduced control effect, and reduced wheat yield, and achieve the effect of promoting synthesis

Active Publication Date: 2018-09-28
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly infects ears of wheat, leading to severe reduction in wheat yield
In addition, Gibberella can also produce mycotoxins, which are directly stored in wheat grains along with the infected pathogenic bacteria, seriously threatening human health
Due to the long-term use of chemical preparations, such as carbendazim, to prevent and control head blight, the pathogens have developed drug resistance, the control effect has decreased, and the ecological environment problems caused by chemical preparation pollution have to be taken seriously

Method used

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  • Multi-functional Streptomyces amritsarensis and application thereof
  • Multi-functional Streptomyces amritsarensis and application thereof
  • Multi-functional Streptomyces amritsarensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Isolation and screening of embodiment 1 bacterial strain

[0039] (1) Isolation of actinomycetes: Collect 200g of red soil from the Chongqing Purple Soil Monitoring Station, take 10g of soil and resuspend it in 90ml of sterile water, apply Gao’s No. 1 medium after gradient dilution, and cultivate at 28°C until a single colony grows According to the difference in the color and colony shape of sporocydia and hyphae in the base, different actinomycetes were purified and preserved.

[0040] (2) Screening of actinomycetes that antagonize Fusarium graminearum: Inoculate the bacterium block of Fusarium graminearum in the center of the PDA solid medium, inoculate actinomycetes around it, observe the antagonistic circle after cultivating at 28° C. for 3 days, and select cells that can Actinomycetes that antagonize Fusarium graminearum actinomycetes to produce antagonistic circles are screened for alginolytic bacteria.

[0041] (3) Screening of alginolytic actinomycetes: Inocula...

Embodiment 2

[0045] The cultivation of embodiment 2 bacterial strains

[0046] Seed medium, Gao's No. 1 medium: soluble starch 20g, KNO 3 1.0g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, add deionized water to 1L, agar powder 2% (m / v, add when solid), pH7.4-7.6.

[0047] Fermentation medium, Luria-Bertani (LB) medium diluted 5 times: tryptone 2g, yeast extract 1g, NaCl 1g, agar powder 2% (m / v, add when solid), deionized water to 1L , pH6.8-7.0.

[0048] Antagonist PDA medium: 200g potatoes, add water and boil for 30 minutes, filter with 2 layers of gauze, take the filtrate for medium preparation, glucose 20g, agar powder 2% (m / v, add when it is solid), add deionized water to 1L , pH natural.

[0049] Algae medium BG11 medium (Blue-Green Medium): NaNO 3 1.5g, K 2 HPO 4 0.04g, MgSO 4 ·7H 2 O 0.075g, CaCl 2 ·7H 2 O 0.036g, Na 2 CO 3 0.02g, citric acid 0.006g, ferric citrate 0.006g, EDTANa 2 .001g, trace element solution A5 1ml (A5:H 3 BO 3 ...

Embodiment 3

[0054] The identification of embodiment 3 bacterial strains

[0055] The strain is characterized as follows:

[0056] Colony morphology: The strain HG-16 grows on the modified Gaoshi No. 1 medium, producing white aerial hyphae and gray-pink spores. After prolonged culture, the colony turns yellow when it ages, and does not produce water-soluble pigments. Microscopic observation The spores are linear rods (see figure 1 a); On LB medium and PDA and other nutrient-rich medium, the colony is wrinkled, light yellow, does not form white aerial hyphae, does not produce spores, and forms basal hyphae closely connected with the medium , all the colonies could not be picked.

[0057] Cultivate Streptomyces alginolyticus with the above-mentioned seed liquid medium for 3 days, then collect the thalline by centrifugation at 10000×g, discard the supernatant, extract the total DNA of the thalline cells with a kit (Fast DNA SPIN kit, USA), and then use primers for PCR Amplified sequence, f...

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Abstract

The invention relates to the field of micro-organisms, and particularly relates to a multifunctional Streptomyces amritsarensis and application thereof. Streptomyces amritsarensis HG-16, with a preservation number of CCTCC-M-2018237. The streptomycin cannot only kill the microcystin, but also antagonize the fusarium graminearum. The fermentation liquid and the active secretion can effectively inhibit the production of the microcystin in a short time, can inhibit the expression of the synthetic gene of 90% microcystin within 9 hours, and can completely inhibit the expression of the microcystingene after 36 hours. Three days after the addition of the Streptomyces, the inhibition rate of the algae toxin is 93%; while for the algicidal bacteria which has been reported to have the effect of inhibiting the production of the microcystin needs more than 3 days to inhibit the synthesis of the microcystin.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the multifunctional alginolytic Streptomycesamritsarensis and application thereof. Background technique [0002] In recent years, with the development of society and economy, human life and production activities discharge a large amount of nutrients such as nitrogen and phosphorus into natural water bodies, resulting in the large freshwater lakes, rivers and coastal coasts in my country, as well as small landscape water bodies. Moderate to severe water eutrophication occurred. It is estimated that more than 75% of the freshwater lakes in the world are in different degrees of eutrophication. The eutrophication of the water body leads to the abnormal proliferation of some harmful dominant algae and the formation of algae blooms, resulting in unpleasant odors in the water body, a decrease in transparency, a decrease in dissolved oxygen, and a large number of aquatic organisms dying, which se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C02F3/34C12R1/465
CPCA01N63/00A01N63/10C02F3/341C12N1/205C12R2001/465
Inventor 代先祝于燕曾予昳罗锋杨彩云张晓辉
Owner SOUTHWEST UNIV
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