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Extraction method of polysaccharide from potentilla discolor

An extraction method, polysaccharide extraction technology, applied in the field of biomedicine, can solve the problem of low polysaccharide purity and achieve the effect of reducing blood sugar content

Inactive Publication Date: 2011-10-26
HAINAN STAND BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, water extraction and alcohol precipitation are mostly used to extract polysaccharides from P. chinensis, and the purity of polysaccharides is low.

Method used

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  • Extraction method of polysaccharide from potentilla discolor

Examples

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Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The method for extracting the polysaccharides from P. vernix provided by the present invention

[0029] Take the dried Herba basilisk medicinal material and grind it to obtain the Herba basilisk powder. Accurately weigh 1.0 kg of Vernaceae powder, add 10.0 L of water and stir evenly, soak for 30 minutes, add hydrochloric acid with a mass concentration of 4.5% dropwise to adjust the pH to 3.8, heat to 55°C, maintain the pH to 3.8, and turn over per kg Ratio of adding 40,000 IU of cellulase and 40,000 IU of pectinase per kg of white herbal medicinal material Add 40,000 IU of cellulase and 40,000 IU of pectinase, stir well, and maintain pH to 3.8, hydrolyzed at 55°C for 3h. After enzymatic hydrolysis, heat to 100°C for 10 minutes to inactivate the enzyme, then cool down to room temperature, and ultrasonicate for 50 minutes under the condition of 2000w. After ultrasonic treatment, heat to 75°C, extract for 3 hours, then cool down to room temperature, centrifuge,...

Embodiment 2

[0038] Example 2 The method for extracting polysaccharides from P. vernix provided by the present invention

[0039] Take the dried Herba basilisk medicinal material and grind it to obtain the Herba basilisk powder. Accurately weigh 1.0kg of Vernaceae powder, add 10.0L of water and stir evenly, after soaking for 30min, add dropwise citric acid with a mass concentration of 4.5% to adjust the pH value to 3.5, heat to 45°C, and maintain the pH value to 3.5. The ratio of adding 40,000 IU of cellulase and 40,000 IU of pectinase to the medicinal material of Bibacao medicinal material was added to the ratio of 40,000 IU of cellulase and 40,000 IU of pectinase, and stirred well, Maintain the pH to 3.5 and hydrolyze for 4 hours at 45°C. After enzymolysis, heat up to 100°C for 10 minutes to inactivate the enzyme, then cool down to room temperature, heat to 80°C for 20 minutes under the condition of 5000w, extract for 4 hours, then cool down to room temperature, centrifuge and filter to...

Embodiment 3

[0048] Example 3 The method for extracting the polysaccharides from P. officinalis provided by the present invention

[0049] Take the dried Herba basilisk medicinal material and grind it to obtain the Herba basilisk powder. Accurately weigh 1.0kg of Vernaceae powder, add 20.0L of water and stir evenly, after soaking for 30 minutes, add phosphoric acid with a mass concentration of 4.5% dropwise to adjust the pH value to 3.4, heat to 50°C, maintain the pH to 3.4, and turn over per kg Ratio of adding 20,000 IU of cellulase and 20,000 IU of pectinase per kg of white herbal medicinal material Add 20,000 IU of cellulase and 20,000 IU of pectinase, stir well, and maintain pH to 3.4, hydrolyzed at 50°C for 4 hours. After enzymatic hydrolysis, heat to 100°C for 10 minutes to inactivate the enzyme, then cool down to room temperature, and ultrasonicate for 90 minutes under the condition of 500w. After ultrasonic treatment, heat to 78°C, extract for 5 hours, then cool down to room temp...

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Abstract

The invention relates to the technical field of biological medicine, and more specifically relates to an extraction method of polysaccharides from potentilla discolor. According to the method, the technology of biological enzymatic hydrolysis is employed to extract polysaccharides from potentilla discolor; the processes of ultrasonic treatment, ultrafiltration, alcohol precipitation, quaternary ammonium salt deposition, salt wash and refining by alcohol precipitation or macroporous resin column are also used to obtain potentilla discolor polysaccharides with high purity. The purity of the obtained potentilla discolor polysaccharides is in a range of 86.28 to 96.27%, and molecular weight of the polysaccharides is 56308 to 68720D. According to tests of drug effects on animals, the potentilla discolor polysaccharides obtained in the invention can substantially reduce content of blood sugar, having a hpyerglycemic effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for extracting polysaccharides from P. vernix. Background technique [0002] Pulsatilla, also known as Pulsatilla, is the whole plant with roots of Potentilla discolor Bunge in the Rosaceae family. It is distributed all over the country and grows in hills, mountains, roadsides and depressions. It is a traditional Chinese medicine in my country. According to the "Compendium of Materia Medica", it has the functions of clearing heat, cooling blood, detoxifying, stopping bleeding and reducing swelling, and is mostly used to treat dysentery, malaria, carbuncle and various bleeding. Modern medicine has shown that Herba Verbacula has the functions of stopping bleeding, stopping dysentery, detoxifying, lowering blood sugar and lowering blood fat, and is an important Chinese herbal medicine plant. [0003] Vermicelli is rich in polysaccharides, dianthus aglycone, D-catechin,...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02A61K31/715A61P3/10
Inventor 韩金光吴家强伍曾利
Owner HAINAN STAND BIO TECH
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