Antrodia camphorata cyclohexenone compound for inhibiting colorectal cancer tumor cell growth

A technology of tumor cells and compounds, applied in new application fields of compounds, able to solve unpublished specific problems

Active Publication Date: 2011-11-09
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its components have been analyzed one after another, but no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomea. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cinnamomum anticancer, and the application of Antrodia Cinnamomea to cancer such as colorectal cancer It is of great help in the treatment and prevention of

Method used

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  • Antrodia camphorata cyclohexenone compound for inhibiting colorectal cancer tumor cell growth
  • Antrodia camphorata cyclohexenone compound for inhibiting colorectal cancer tumor cell growth
  • Antrodia camphorata cyclohexenone compound for inhibiting colorectal cancer tumor cell growth

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.

[0025] The previously collected Antrodia camphorata extract was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP 18 chromatographic tube (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution ( B) as mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is...

Embodiment 2

[0028] Anti-tumor cell activity test of colorectal cancer in vitro

[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium containing human colorectal cancer tumor cells WiDr, to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the known MTT analysis method, and the colorectal cancer tumor cell line WiDr is a human colorectal cancer cell line (human colon adenocarcinoma cell line).

[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromid...

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Abstract

The invention relates to a new application of a compound and a medicinal composition for inhibiting colorectal cancer tumor cell growth. 4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone is separated and purified from antrodia camphorata extract. The cyclohexenone compound can be applied to inhibition of colorectal cancer tumor cell growth, and also can beapplied to medicinal compositions for inhibiting colorectal cancer tumor cell growth.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from the extract of Antrodiacinnamomea to inhibit the growth of colorectal cancer tumor cells. Background technique [0002] The large intestine includes the cecum, colon and rectum, which is the last checkpoint of the human digestive system. The normal colorectal mucosa will cause benign cells to proliferate on the inner wall of the large intestine due to many factors to form adenoma (ie, deflated flesh), and even further become colorectal cancer. Colorectal cancer, also known as colorectal cancer, is one of the most common malignant tumors in the world. In recent years, in Taiwan, due to the westernization and refinement of Chinese people's life and diet, as well as the increase in animal fat and protein intake Increasing year by year and decreasing dietary fiber intake, the incidence rate of colorectal cancer is increasing ye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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