Antrodia camphorata cyclohexenone compound for inhibiting colorectal cancer tumor cell growth
A technology of tumor cells and compounds, applied in new application fields of compounds, able to solve unpublished specific problems
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Embodiment 1
[0023] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate
[0024] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.
[0025] The previously collected Antrodia camphorata extract was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP 18 chromatographic tube (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution ( B) as mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is...
Embodiment 2
[0028] Anti-tumor cell activity test of colorectal cancer in vitro
[0029] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium containing human colorectal cancer tumor cells WiDr, to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by the known MTT analysis method, and the colorectal cancer tumor cell line WiDr is a human colorectal cancer cell line (human colon adenocarcinoma cell line).
[0030] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromid...
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