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Fermentation medium and fermentation method of producing A40926 with Nonomuraea.sp by fermentation

A technology of Actinomyces Nonomura, A40926, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low fermentation unit, and achieve the effect of increasing fermentation unit and increasing yield

Inactive Publication Date: 2014-08-27
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to provide a new fermentation medium for the production of antibiotic A40926 by fermentation of Actinomyces Nonomura for the low fermentation unit of antibiotic A40926 produced by Actinomyces Nonomura. And the corresponding fermentation method, which can greatly increase the fermentation unit of A40926 and improve production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take a strain of Actinomycetes Nonomuraea (Nonomuraea.sp) ATCC39727 and streak it on the seed slant medium, culture it at 30°C for 7 days, and then inoculate it in the shake flask seed medium. The liquid volume is 20ml / 250ml. Cultured on a shaker for 72 hours. The seed liquid was inoculated with 10% (v / v) inoculum amount in a 250 ml fermentation shaker flask with a liquid filling volume of 20 ml for fermentation, the fermentation temperature was 30° C., and the fermentation period was 5 days. After the fermentation, adjust the pH of the fermentation broth to 12.0 with NaOH, incubate at 50°C for 1 hour, and then centrifuge to take the supernatant. HPLC assay shows that the yield of A40926 reaches 366 mg / L.

[0033] The composition of the slant medium used therein: ISP2 medium, the components are glucose 4.0g / L, yeast extract powder 4.0g / L, malt extract powder 10.0g / L, agar powder 20g / L, pH7.3.

[0034] The composition of the seed medium used: ISP2 medium, the components...

Embodiment 2

[0037] Fermentation medium composition: glucose 10.0g / L, dextrin 5.0g / L, yeast extract powder 10.0g / L, hot-pressed soybean cake powder 20.0g / L, NaCl 2.0g / L, pH to 8.0.

[0038] The seeds prepared in Example 1 were inoculated in the fermentation medium with a 10% (v / v) inoculum size, the fermentation temperature was 30° C., and the fermentation period was 7 days. Other conditions were the same as in Example 1. The yield of A40926 reached 98mg / L.

Embodiment 3

[0040] Composition of fermentation medium: glucose 10.0g / L, dextrin 5.0g / L, yeast extract powder 10.0g / L, hot-pressed soybean cake powder 20.0g / L, valine 1.0g / L, NaCl 2.0g / L, pH to 8.0.

[0041] The seeds prepared in Example 1 were inoculated in the fermentation medium with a 10% (v / v) inoculum size, the fermentation temperature was 30° C., and the fermentation period was 7 days. Other conditions were the same as in Example 1. The yield of A40926 reached 180mg / L.

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Abstract

The invention provides a fermentation medium and a corresponding fermentation method in favor of efficiently producing an antibiotic A40926 with Nonomuraea.sp by fermentation. The fermentation medium comprises a carbon source and a nitrogen source, wherein, the carbon source is an organic carbon source with the content of 5-180g / L, the nitrogen source is an organic nitrogen source with the content of 2-210g / L, and the contents are measured as the total volume of the medium. By optimizing the components and ratio of carbon and nitrogen source of the fermentation medium, the cultural temperature, PH value and other fermentation parameters are optimized, so that the output of the A40926 is greatly improved. According to the method provided by the invention, the fermentation unit of the A40926 is greatly improved, so that the method is suitable for large-scale production of the A40926.

Description

technical field [0001] The invention belongs to the technical field of industrial microorganisms, and in particular relates to a novel fermentation medium for producing antibiotic A40926 by Nonomura actinomycetes and a corresponding fermentation method. Background technique [0002] In 1984, scientists discovered the antibiotic A40926 while cultivating Actinomadura strains isolated from soil. And on June 8, 1984, the strain was deposited in the American Tissue Culture Collection (American Tissue Culture Collection, ATCC), numbered ATCC39727. The strain was initially considered to belong to the genus Streptomyces, and after further research, especially on the synthesis of cell walls, in 2003, its producing bacteria were classified as Nonomuraea.sp. A40926 belongs to the vancomycin class of antibiotics and has the ability to bind acyl-D-alanyl-D-alanine. It has good antibacterial effect on Gram-positive bacteria and Neisseria gonorrhoeae (Neisseria gonorrhoeae), and has a lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00C12R1/01
Inventor 陈少欣王岩沈晓放
Owner SHANGHAI INST OF PHARMA IND CO LTD
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