Bacillus subtilis preparation for tobacco stem treatment

A technology for microbial preparations and bacteria, applied in the field of microbial preparations, can solve problems that have not yet been discovered, and achieve the effects of reduced irritation, strong controllability, and good development and application prospects.

Inactive Publication Date: 2011-11-23
HONGYUN HONGHE TOBACCO (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ——Test tube slant culture

[0026] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Van35, culture at 28°C for 24 hours, and obtain test tube species.

Embodiment 2

[0028] - Seed cultivation

[0029] A Luria-Bertani (LB) medium was used, and the formula was: 10 g of peptone, 10 g of sodium chloride, 5 g of yeast extract, and distilled water to a volume of 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate the Van35 test tube species and culture at 28°C for 12 hours to obtain liquid strains.

Embodiment 3

[0031] - Preparation of microbial preparations

[0032] Take the liquid strain, inoculate it into 200ml of LB liquid medium according to the inoculum amount of 0.1%, and cultivate it in a shaker at 37°C with a rotation speed of 180r / min until the OD value = 1.5. Centrifuge at 3500rpm / min for 10min, and wash with sterile water Precipitate, centrifuge, then oscillate evenly with 20mL sterile water, and dilute 10 times when used to be the bacterial agent.

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PUM

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Abstract

The invention discloses a microbial preparation for tobacco, a preparation method and an application thereof, and belongs to the technical field of additives. According to the invention, liquid fermentation culture of a producing strain is adopted to obtain the corresponding microbial preparation. The producing strain is bacillus subtilis (Van3), and the strain was preserved in general microbiological culture collection center of China committee for culture collections of microorganisms in Aug 16th, 2010, and has a preservation number of CGMCC No.4091. The microbial preparation of the invention can obviously improve the stem quality such as aroma volume, cheerfulness, and the like, reduces miscellaneous gas and irritation, decreases smoke concentration and strength, makes aftertaste become comfortable, and thus further increases the mixing amount of stems in a cigarette so as to realize the purposes of harm reduction, tar reduction, and cigarette production cost reduction.

Description

technical field [0001] The invention belongs to the technical field of cigarette additives, and more specifically, the invention relates to a microbial preparation. At the same time, it also relates to the preparation method of the microbial preparation and its application in the tobacco industry. Background technique [0002] With the improvement of my country's cigarette technology level and the continuous development of harm reduction and tar reduction in the tobacco industry, expanded cut stems are more widely used in cigarette formulations. After adding expanded shredded stems to the formula, due to its high filling value, the amount of shredded tobacco in cigarettes can be reduced, which not only saves the cost of cigarettes, but also reduces the amount of tar in cigarettes. [0003] However, the current technical level of comprehensive utilization of tobacco stem resources is relatively backward, the waste of idle tobacco stems is large, the blending ratio of shredde...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/125
Inventor 陈兴曾晓鹰段焰青王坚黄立斌廖头根王家俊何靓唐丽
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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