Immunochromatographic test paper for detecting enrofloxacin and preparation method thereof

An immunochromatographic test strip and enrofloxacin technology, which can be used in measurement devices, analytical materials, instruments, etc., can solve the problems of long color development time, limited superparamagnetic composite particles, and easy polymerization of magnetic beads.

Inactive Publication Date: 2014-03-19
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation and modification methods are often cumbersome and complicated, and the obtained superparamagnetic composite particles cannot meet the requirements of LFIAs in terms of size, biocompatibility, saturation magnetic strength, external magnetic field response speed, stability, and labeling efficiency. The size is mostly between 200 and 300nm. Due to the large size of the magnetic beads, the swimming time on the test paper is slow and the color development time is long; and the particles that are too small cannot provide sufficient magnetic resonance signals; in addition, there are biological Unstable compatibility and easy aggregation of magnetic beads; these shortcomings limit the application of superparamagnetic composite particles in LFIAs

Method used

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  • Immunochromatographic test paper for detecting enrofloxacin and preparation method thereof
  • Immunochromatographic test paper for detecting enrofloxacin and preparation method thereof
  • Immunochromatographic test paper for detecting enrofloxacin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1, Preparation of magnetically labeled rapid detection test paper for enrofloxacin residues

[0090] (1) Preparation of enrofloxacin antibody labeled with superparamagnetic composite particles

[0091] The particle size is 100nm, the magnetic saturation intensity is 40emu / g, the corresponding external magnetic field response speed is 20 seconds, and the superparamagnetic composite particle (superparamagnetic Fe 3 o 4 Nanoparticles) (purchased from Shenzhen Thales Technology Co., Ltd., catalog number MP-2) labeled enrofloxacin antibody.

[0092] The specific method is:

[0093] 1) Take 2.5mg of the above-mentioned magnetic particles, wash them with 0.1M MES buffer (weigh 1.066g MES, 0.45g NaCl and dissolve them in 50ml pure water, adjust the pH to 4.7), separate and enrich them with a 0.4T magnetic rack, and use 1ml of MES buffer with a concentration of 0.1M and a pH value of 4.7 was resuspended, and 0.96mg (final concentration of 5mM) of 1-ethyl-(3-dimethylam...

Embodiment 2

[0108] Example 2, detection of the sensitivity, cross-reactivity and accuracy of the immunochromatographic test paper for enrofloxacin residues.

[0109] 1. Determination of sensitivity

[0110] Weigh an appropriate amount of enrofloxacin (Beijing Hengyuan Qitian Chemical Technology Research Institute, catalog number: 30235CDCT-C13170000), and dissolve it in 0.01M sodium hydroxide solution to prepare a standard stock solution of 100ug / ml. Dilute with 0.02M PBS (pH=7.4) to prepare standard solutions of 0, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5, 10ug / L, and add the detection Enroza obtained in Example 1 respectively Star's immunochromatographic test paper, and read with superparamagnetic resonance detector MAR (Magna BioSciences, 8094-101-01 & 8094-101-02). Detection steps: Return the sample to be tested to room temperature (25°C) before detection, use a precise pipette to take 100 μl of the sample to be tested and drop it vertically and slowly into the sample loading end of ...

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Abstract

The invention discloses immunochromatographic test paper for detecting enrofloxacin and a preparation method thereof. The immunochromatographic test paper for detecting the enrofloxacin comprises a sample pad containing an enrofloxacin antibody labeled by superparamagnetic composite particles, a nitrocellulose membrane connected at one end of the sample pad and a water-absorbing pad connected at the other end of the nitrocellulose membrane, wherein the nitrocellulose membrane is coated with a detection line and a quality control line, which are separated mutually, the detection line contains an enrofloxacin antigen and the quality control line contains an anti-antibody which can be specifically combined with the enrofloxacin antibody. Experiments prove that, by using the test paper provided by the invention for detecting the enrofloxacin, the sensitivity is high, the specificity is strong, the test is fast and simple, and the objective determination can be realized.

Description

technical field [0001] The invention relates to a reagent for detecting enrofloxacin residues, in particular to an immunochromatographic test paper for detecting enrofloxacin and a preparation method thereof. Background technique [0002] Enrofloxacin (EF) is a special fluoroquinolone FQs drug for animals. It is a synthetic broad-spectrum bactericidal antibacterial drug. It is widely used in aquatic products and livestock and poultry farming. It has become a commonly used fishery drug in my country and one of veterinary drugs. In recent years, there are more and more reports about the drug resistance, residual toxicity and adverse reactions of FQs drugs in clinical application, and the detection of corresponding residues in food has also attracted more and more attention. my country is a big country in the production, consumption and export of aquatic products, and enrofloxacin is one of the drug residues that are mainly monitored for aquatic products in recent years. At pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/532G01N33/531
Inventor 马岚袁航吴峰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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