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Immunochromatography test paper for detecting Ciprofloxacin and preparation method thereof

A technology of immunochromatography test paper and ciprofloxacin, which is applied in immunoglobulin, chemical instruments and methods, analytical materials, etc., can solve the problems of insufficient magnetic resonance signal, easy aggregation of magnetic beads, long color development time, etc.

Inactive Publication Date: 2014-03-19
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation and modification methods are often cumbersome and complicated, and the obtained superparamagnetic composite particles cannot meet the requirements of LFIAs in terms of size, biocompatibility, saturation magnetic strength, external magnetic field response speed, stability, and labeling efficiency. The size is mostly between 200 and 300nm. Due to the large size of the magnetic beads, the swimming time on the test paper is slow and the color development time is long; and the particles that are too small cannot provide sufficient magnetic resonance signals; in addition, there are biological Unstable compatibility and easy aggregation of magnetic beads; these shortcomings limit the application of superparamagnetic composite particles in LFIAs

Method used

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  • Immunochromatography test paper for detecting Ciprofloxacin and preparation method thereof
  • Immunochromatography test paper for detecting Ciprofloxacin and preparation method thereof
  • Immunochromatography test paper for detecting Ciprofloxacin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1, Preparation of magnetically labeled rapid detection test paper for ciprofloxacin residues

[0088] (1) Preparation of ciprofloxacin antibody labeled with superparamagnetic composite particles

[0089] The particle size is 100nm, the magnetic saturation intensity is 40emu / g, the corresponding external magnetic field response speed is 20 seconds, and the superparamagnetic composite particle (superparamagnetic Fe 3 o 4 Nanoparticles) (purchased from Shenzhen Thales Technology Co., Ltd., catalog number MP-2) labeled ciprofloxacin antibody.

[0090] The specific method is:

[0091] 1) Take 2.5mg of the above-mentioned magnetic particles, wash them with 0.1M MES buffer (weigh 1.066g MES, 0.45g NaCl and dissolve them in 50ml pure water, adjust the pH to 4.7), separate and enrich them with a 0.4T magnetic rack, and use 1ml of MES buffer with a concentration of 0.1M and a pH value of 4.7 was resuspended, and 0.96mg (final concentration of 5mM) of 1-ethyl-(3-dimethy...

Embodiment 2

[0106] Embodiment 2, the detection of the sensitivity, cross-reaction and accuracy of the immunochromatographic test paper of ciprofloxacin residue.

[0107] 1. Determination of sensitivity

[0108] An appropriate amount of ciprofloxacin (Beijing Hengyuan Qitian Chemical Technology Research Institute, catalog number: 30233CDCT-C11668500) was weighed and dissolved in 0.01M sodium hydroxide solution to prepare a standard stock solution of 100ug / ml. Dilute and prepare standard solutions of 0, 0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5, 10 ug / L with 0.02M PBS (pH=7.4), add the detection ciprosa obtained in Example 1 respectively Star's immunochromatographic test paper, and read with superparamagnetic resonance detector MAR (Magna BioSciences, 8094-101-01 & 8094-101-02). Detection steps: Return the sample to be tested to room temperature (25°C) before detection, use a precise pipette to take 100 μl of the sample to be tested and drop it vertically and slowly into the sample loading ...

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Abstract

The invention discloses immunochromatography test paper for detecting Ciprofloxacin and a preparation method thereof. The immunochromatography test paper for detecting the Ciprofloxacin comprises a sample pad, a nitrocellulose membrane and an absorbent pad, wherein the sample pad contains a superparamagnetic compound particle marker Ciprofloxacin antibody; the nitrocellulose membrane is connected to one end of the sample pad; the absorbent pad is connected to the other end of the nitrocellulose membrane; a detection line and a quality control line which are separated with each other are coated on the nitrocellulose membrane; the detection line contains a Ciprofloxacin antigen; and the quality control line contains an antiantibody which can be specifically bound with the Ciprofloxacin antibody. Proved by the experiment of the invention, the test paper for detecting the Ciprofloxacin provided by the invention has high sensitivity, strong specificity and is rapid, simple and convenient, and objective determination can be realized.

Description

technical field [0001] The invention relates to a detection reagent for ciprofloxacin residues, in particular to an immunochromatographic test paper for detecting ciprofloxacin and a preparation method thereof. Background technique [0002] Quinolones (QNS) drugs are a class of very important broad-spectrum antibiotics that have been rapidly developed in the past 20 years. They can inhibit bacterial DNA helicase, have a broad antibacterial spectrum, high efficiency, low toxicity, and strong tissue penetration. It has become one of the most important anti-infective drugs in veterinary clinics and aquaculture, and has been widely used in treatment, prevention and growth promotion, and has attracted widespread attention due to its drug resistance and potential carcinogenicity. In tissues, the marked residues of enrofloxacin are enrofloxacin and ciprofloxacin, among which the residue concentration in liver tissue and kidney tissue is the highest, followed by muscle and fat attac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558C07K16/44
Inventor 马岚袁航吴峰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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