A kind of alginate feed additive and alginate feed

A feed additive and alginate technology, applied in the field of feed, to achieve the effect of reduced production costs and wide application

Inactive Publication Date: 2011-12-21
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, alginate, as a growth-promoting factor for bifidobacteria, has not been used in feed

Method used

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  • A kind of alginate feed additive and alginate feed
  • A kind of alginate feed additive and alginate feed
  • A kind of alginate feed additive and alginate feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of Azotobacter vinelandii alginate: add 1 μl of Azotobacter vinelandii to 20ml of nitrogen-free liquid medium, culture on a shaker at 26-30°C for 4 days, and then spread 1.5ml of the culture evenly for transplantation Into the plate culture medium, incubator 26-30 ℃ for 2-3 days, 20ml bacterial solution can coat 13 medium-sized petri dishes. Scrape all cultures, wash with normal saline, then extract with isopropanol at 4°C, wash with normal saline, centrifuge, and freeze-dry at -20~-80°C to obtain brown nitrogen-fixing bacteria alginate, the obtained alginate The average yield was 0.1026 g / gCDM, that is, 0.1126 grams of alginate per gram of cell dry matter.

[0020] (2) Prepare alginate feed additives (here refers to additives not feed): lysine 10,000 mg, methionine 10,000 mg, selenium 12.5-25 mg, vitamin A 6 million IU, vitamin D 3 6 million IU, 200 mg of antioxidant (ethoxyquinoline), mix well, add the brown nitrogen-fixing bacteria alginate in step (...

Embodiment 2

[0046] (1) Preparation of Azotobacter vinelandii alginate: add 1 μl of Azotobacter vinelandii to 20ml of nitrogen-free liquid medium, culture on a shaker at 26-30°C for 4 days, and then spread 1.5ml of the culture evenly for transplantation Into the plate culture medium, incubator 26-30 ℃ for 2-3 days, 20ml bacterial solution can coat 13 medium-sized petri dishes. Add 2000ml of water respectively, 80°C water bath for 8h, centrifuge for 10min to concentrate the supernatant to 1 / 4 volume, add 3 times the amount of 95% ethanol to the concentrate, leave overnight, discard the supernatant, centrifuge at 3000rpm for 10min, and vacuum-dry the obtained precipitate to obtain brown nitrogen fixation Bacterial alginate, the average yield of alginate is 0.1089g / gCDM, that is, 0.1089 grams of alginate per gram of cell dry matter.

[0047] (2) Prepare alginate feed additives: lysine 10,000 mg, methionine 10,000 mg, selenium 12.5-25 mg, vitamin A 6 million IU, vitamin D 3 6 million IU, 200 ...

Embodiment 3

[0049] Embodiment 3: adopt the method for embodiment 1 to prepare brown nitrogen-fixing bacteria alginate, add brown nitrogen-fixing bacteria alginate in common feed additive so that its mass percentage is 2.5% to obtain alginate feed additive, in common routine The alginate feed additive is added to the feed formula so that the mass percentage is 6% to obtain the alginate feed.

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Abstract

The invention discloses an alginate feed additive and an alginate feed. The feed additive contains alginate, and the alginate feed contains 6-9% (by weight) of the alginate feed additive. The invention will As a feed additive, alginate is used in feed to provide rich bifidobacteria growth factors for the growth of animals, which has obvious weight gain effect on animal growth, and is safe and reliable. The use of alginate obtained from bacterial culture enables production The cost is greatly reduced, and it can be widely used in feed to reduce the cost of raising livestock and poultry.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to an alginate feed additive and alginate feed. Background technique [0002] In the 1970s, Professor Mitsuobo of the University of Tokyo discovered that functional oligosaccharides are a growth-promoting factor for bifidobacteria, which can especially promote the rapid proliferation of beneficial bacteria such as bifidobacteria in the intestinal tract of animals, which is beneficial to the health of humans and animals. Alginate is a functional oligosaccharide, which is usually extracted from natural marine brown algae. However, alginate extracted from natural marine brown algae requires a large amount of raw materials, resulting in increased costs. Therefore, it has broad development potential to be produced by bacteria. Alginate produced by bacteria (also known as alginate) has similar functions and effects to alginate extracted from natural marine brown algae (Rehm, B.H.A., and S.Va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K10/16A23K10/26A23K10/30A23K10/37A23K20/22A23K20/26
CPCY02P60/87
Inventor 杨茂生梁应林李红曙
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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