34 results about "Azotobacter vinelandii" patented technology

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention discloses a complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting and a preparation method thereof. The preparation is mainly prepared by mixing the following raw materials in parts by weight: 5-20 parts of azotobacter vinelandii, 10-30 parts of bacillus megatherium, 10-30 parts of bacillus mucilaginosus, 5-15 parts of azotobacter chroococcum, 10-15 parts of glomus intraradices and 10-25 parts of glomus mosseae. The preparation method comprises the following steps: respectively carrying out high-density culture on each strain; grafting to a shake flask filled with a culture medium in a volume ratio of 15-30 percent for activated culture; grafting the cultured strains into a fermentation tank for fermentation culture expansion; dehydrating and drying the culture expanded single strains to prepare hypopus microorganism dried powder; and mixing to obtain the complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting. The complex bacterium preparation is used for industrial crops, fruit trees, food crops and the like, has the advantages of biological property, environmental friendliness, high yield and high quality in comparison with existing products, and cannot pollute soil.

The invention discloses a special complex microbial agent for harmless treatment of livestock and poultry excrements. The special complex microbial agent is prepared from the following raw materials in parts by weight: 0.02-0.04 part of bacillus mucilaginosus, 0.01-0.03 part of bacillus licheniformis, 0.01-0.03 part of streptomyces jingyangensis whey powder, 0.01-0.02 part of azotobacter vinelandii, 0.04-0.08 part of aspergillus spore powder, 0.01-0.03 part of streptococcus thermophilus, 0.01-0.02 part of enterococcus faecalis, 0.02-0.04 part of antimicrobial peptide thallus, 80.0-120.0 parts of dried silkworm dropping, 2.0-6.0 parts of peanut cakes, 2.0-3.0 parts of bran, 7.0-8.0 parts of starch, 3.0-3.5 parts of molasses, 2.0-3.0 parts of monocalcium phosphate, and 1.0-1.4 parts of urea. Used for harmless treatment of livestock and poultry excrements, the special complex microbial agent has the advantages that the fermentation time is short, the quality of a fertilizer is high, the active rate of microorganisms in soil can be activated, the temperature permeability of air in soil can be increased, and the special complex microbial agent has an effect of preventing and controlling various fungal diseases and bacterial diseases, as well as effects of increasing the yield and improving the quality while providing complete nutrients for the crop, thus having better economic benefits, social benefits and ecological benefits.

The invention discloses a pest-repelling biological organic fertilizer for dragon fruit trees, and belongs to the technical field of preparation of fertilizers. The pest-repelling biological organic fertilizer for dragon fruit trees comprises a tobacco fertilizer, Nerium indicum leaves, onion, Crotalaria mucronata, tea leaves, barnyard manure, bagasse, plant ash, a cake fertilizer, animal manure, household garbage, ammonium dihydrogen phosphate, Aspergillus oryzae, yeast, Bacillus subtilis, mycorrhizal fungi, Azotobacter vinelandii, photosynthetic bacteria and Paecilomyces lilacinus. The tobacco fertilizer, Nerium indicum leaves, onion, Crotalaria mucronata and tea leaves having a pest repelling function are added to the pest-repelling biological organic fertilizer, so the stress resistance of the dragon fruit trees is improved, grubs, mole crickets, termites, ground nematodes and other dragon fruit tree plantation common pests are effectively dispelled, the use of pesticides is reduced, and the quality of dragon fruits is improved.

The invention relates to a method for performing raw soil greening on coast saline land through a biological modifier containing fly ash. The biological modifier comprises the following components in weight part: 8-12 parts of ceramsite, 11-15 parts of vermiculite, 1-5 part of fly ash, 0.5-2 parts of iron chloride, 0.5-2 parts of calcium dihydrogen phosphate, 0.2-1 parts of formic acid, 1-4 parts of amino acid salt, 2-6 parts of fermented sheep manure, 2-7 parts of capsicum straw powder, 0.3-1 parts of compound sodium nitrophenolate, 0.3-1 parts of amino-oxyacetic acid, 0.3-1 parts of soybean germ extract, 0.5-2 parts of potassium ferrocyanide, 0.2-0.5 parts of cellulose cellulose grafted acrylic acid salt, 0.1-0.5 parts of halomonas, 0.1-0.5 parts of Bacillus subtilis, 0.1-0.5 parts of azotobacter vinelandii, 0.1-0.5 parts of Aspergillus oryzae and 0.1-0.5 parts of Trichoderma longibrachiatum.

The invention relates to a method for performing natural soil greening on low-lying saline soil by using a soil conditioner containing chitosan. According to the formula of the soil conditioner, the soil conditioner comprises, by weight parts, 32-34 parts of fermented deer manure, 8-10 parts of sorghum straw powder, 5-7 parts of bentonite, 6-8 parts of biogas residue, 3-5 parts of phosphorite powder, 2-4 parts of chitosan, 2-4 parts of fish bone meal, 1-3 parts of peanut cake, 1-3 parts of egg-shell meal, 2-4 parts of soybean meal, 1-3 parts of ammonium bicarbonate, 1-3 parts of ammonium phosphate, 1-3 parts of ammonium chloride ferric sulfate, 1-3 parts of magnesium hydrogen phosphate, 0.5-0.7 part of phenylacetic acid, 1-3 parts of amino acid salt, 0.2-0.4 part of sodium nitrophenolate, 0.2-0.4 part of brassin, 0.2-0.4 part of gibberellin, 0.1-0.3 part of methanohalophilus zhilinae, 0.1-0.3 part of bacillus coagulans, 0.1-0.3 part of saccharomyces cerevisiae, 0.1-0.3 part of azotobacter vinelandii and 0.1-0.3 part of aspergillus oryzae.

The invention discloses an organic fertilizer for optimizing soil system environment, a preparation method and an application thereof, and relates to the technical field of organic fertilizers. The organic fertilizer includes, by weight, 200000 parts of chicken manure, 20000-40000 parts of vermiculite, 30000-50000 parts of peanut powder, 100-200 parts of a photo-catalyst, 2-4 parts of bacillus subtilis bacterial powder, 2-4 parts of bacillus mucilaginosus bacterial powder, 2-4 parts of bacillus licheniformis bacterial powder, 2-4 parts of mycorrhiza fungi bacterial powder, 2-4 parts of azotobacter vinelandii bacterial powder, 2-4 parts of streptomyces bacterial powder, 2-4 parts of soil actinomycete bacterial powder, 250-350 parts of beauveria bassiana bacterial liquid, 250-350 parts of metarhizium anisopliae bacterial liquid, 250-350 parts of bacillus thuringiensis bacterial liquid and 250-350 parts of lactic acid bacteria liquid, wherein the living bacteria numbers of the bacterial liquids of the beauveria bassiana, metarhizium anisopliae, bacillus thuringiensis and lactic acid bacteria are all not less than 10 billions/ml. The organic fertilizer can increase organic substance content in soil, increase utilization rate and solve a problem of soil caking, can increase yield and improve quality of crops and further increase economic benefit. The organic fertilizer also can prevent and control underground pests and improve growth environment of plants.

The invention relates to the field of fertilizer, in particular to lychee tree planting fertilizer. The lychee tree planting fertilizer comprises, by weight, 2-6 parts of mycorrhiza fungi, 2-4 parts of streptomyces jingyangensis, 1-2 parts of bacillus subtilis, 3-5 parts of azotobacter vinelandii, 0.5-1 part of saccharomycetes, 1-4 parts of phosphobacteria, 35-45 parts of organic fertilizer, 35-45 parts of inorganic fertilizer, 15-20 parts of rice chaff, 8-14 parts of humic acid, 3-7 parts of urea acetaldehyde and 3-9 parts of iso-butylidene diurea. The lychee tree planting fertilizer is stable in performance, wide in function, free from toxic and side effect and good in application prospect; various flora promote and complement mutually, crop output is increased, taste is improved, and succulent and refreshing taste is achieved.

The invention provides a preparation method of a liquid composite bacterial strain. The bacterial strain comprises azotobacteria, potassium decomposing bacterium, and antibiotic bacterium; wherein the azotobacteria is a bacterium selected from the following bacteria: rhizobium, azotobacter chroococcum, C58/PGV3850 strain of agrobacterium tumefaciens, Hainan rapid growing rhizobium 166, azospirillum Yu-62, associative nitrogen-fixing bacteria Klebsiella planticola 19-1 and azotobacter vinelandii Az230; the potassium decomposing bacterium is a bacterium selected from the following bacteria: NBT strain of silicate bacteria, JF88 stain of potassium bacteria, and G10 strain of silicate bacteria; the antibiotic bacterium is a bacterium selected form the following bacteria: streptomycete, penicillium, bacillus polymyxa and 5406 antibiotic bacterium. The preparation method comprises the following steps: separately selecting one bacterium from the azotobacteria group, the potassium decomposing bacterium group and the antibiotic bacterium group mentioned above; individually preparing corresponding bacterial strain culture mediums, wherein the pH values of the culture mediums are in a range of 6 to 8.5; then individually subjecting the three culture mediums to conventional processes of packaging in pots, sterilizing, cooling, inoculating, and culturing so as to obtain liquid bacterial strains; then mixing the three liquid bacterial strains, wherein the weight ratio of the azotobacteria to the potassium decomposing bacterium to the antibiotic bacterium is 1:1:(0.025 to 0.05). The preparation method meets the requirement of production of nutritionally-balanced compound feed, and has the advantages of little investment, easy mastery, easiness, and low cost.

The invention relates to a method for carrying out raw soil greening on secondary salinized saline and alkaline land by using an amino acid salt-containing soil conditioner. The soil conditioner comprises, by weight, 31-33 parts of fermented pigeon manure, 10-13 parts of decomposed rice straw powder, 3-5 parts of fly ash, 3-5 parts of phosphate rock powder, 2-4 parts of bentonite, 2-4 parts of calcium superphosphate, 1-3 parts of manganese hypophosphite, 1-3 parts of adipic acid, 3-5 parts of amino acid salt, 0.5-0.7 parts of compound sodium nitrophenolate, 0.5-0.7 parts of rapin, 0.5-0.7 parts of a wolfberry fruit extract product, 3-5 parts of sodium silicoaluminate, 0.5-0.7 parts of a starch grafted styrene sulfonic acid copolymer, 1-3 parts of ammoniated superphosphate, 1-3 parts of chelated iron, 1-3 parts of chelated zinc, 0.1-0.3 parts of Bacillus amyloliquefaciens, 0.1-0.3 parts of Bacillus megaterium, 0.1-0.3 parts of Bacillus licheniformis, 0.1-0.3 parts of microzyme, 0.1-0.3 parts of Azotobacter vinelandii and 0.1-0.3 parts of Trichoderma Longibrachiatum.

The invention relates to a method for performing raw soil greening on moderate saline land through a soil conditioner comprising rice bran. The method comprises the following steps of selecting the moderate saline land, preparing the soil conditioner comprising the rice bran, applying the soil conditioner comprising the rice bran, and planting green grass after soil improvement. The soil conditioner comprising the rice bran comprises the following components in weight part: 21-23 parts of fermented rabbit manure, 12-14 parts of fermented pigeon manure, 5-7 parts of river sand, 3-5 parts of fly ash, 2-4 parts of zeolite powder, 1-3 parts of rice bran, 1-3 parts of caproic acid, 1-3 parts of amino acid salt, 3-5 parts of disodium hydrogen phosphate, 3-5 parts of sodium phosphate, 3-5 parts of sodium pyrophosphate, 3-5 parts of potash, 0.5-0.7 parts of diethyl aminoethyl hexanoate, 0.5-0.7 parts of forchlorfenuron, 0.5-0.7 parts of mulberry extract, 2-4 parts of microcrystalline cellulose, 0.5-0.7 parts of xanthate cellulose grafted acrylate, 0.1-0.3 parts of Halobacterium salinarium, 0.1-0.3 parts of bacillus megaterium and 0.1-0.3 parts of azotobacter vinelandii.

The invention discloses a preparation method for desert soil micro drip irrigation microbial bacterial manure. The method includes the steps of conducting fermentation in a basic culture medium of one of similar bacterial strains of six types of bacteria including bacillus licheniformis, bacillus subtilis, Potsdam bacillus, bacillus mucilaginosus, rhodopseudomonas palustris and azotobacter vinelandii according to the inoculation quantity of 2.0% at the temperature of 28 DEG C to 37 DEG C and at the speed of 180 r/min for 36 hours to obtain original fermentation fluid of all the bacteria, mixing the six types of original fermentation fluid according to the size ratio of 1 to 1 to 1 to 2 to 2 to 3, and conducting ventilating, stirring and fermenting in a starch aqueous solution for 36 hours to 48 hours at the temperature of 28 DEG C to 34 DEG C till the microorganism content in the culture solution is higher than or equal to 4.0 *108 colony-forming unit (CFU)/mL to obtain the desert soil micro drip irrigation microbial bacterial manure, wherein the starch aqueous solution contains (NH4)SO4 with the density of 2.0 g/L to 4.0 g/L and NaH2PO4 with the density of 1.5 g/L to 3.0 g/L, the pH of the starch aqueous solution ranges from 6.5 to 7.5, and the percentage mass content is 2.0%.

The invention relates to a multifunctional biodegradation enzyme chemical preparation (AVE preparation) generated by fermenting azotobacter vinelandii and phosphate, malysite and molybdate by adopting a microorganism separated from the soil. The method comprises the following steps that: the AVE chemical preparation is directly scattered inside the body fat, protein, pectin, fiber and starch fluid or the body fat, protein, pectin and starch fluid are directly invaded into the AVE chemical preparation to perform the catalytic hydrolysis and degradation. The method is characterized in that the azotobacter vinelandii is fermented inside a culture medium to prepare the AVE chemical preparation which is the multifunctional bio-enzyme preparation with the functions of catalytic hydrolysis of the body fat, protein, pectin, fiber and starch.

The invention relates to a method for carrying out raw soil greening on beach saline and alkaline land by using a denitration waste-containing soil conditioner. The formula of the denitration waste-containing soil conditioner comprises, by weight, 25-27 parts of fermented dog manure, 8-10 parts of green bean skin powder, 2-4 parts of denitration wastes, 3-5 parts of vermiculite, 2-4 parts of river sands, 1-3 parts of rubber powder, 1-3 parts of stearic acid, 1-3 parts of compound sodium nitrophenolate, 1-3 parts of rapin, 1-3 parts of gibberellin, 0.5-0.7 parts of shiitake extract, 0.2-0.4 parts of Mahonia fortunei (Lindl.) Fedde, 1-3 parts of amino acid salts, 2-4 parts of cattle bone meal, 1-3 parts of ferrous sulfate, 2-4 parts of ammonium sulfate, 2-4 parts of calcium superphosphate, 2-4 parts of potassium dihydrogen phosphate, 2-4 parts of ferric pyrophosphate, 3-5 parts of sodium silicoaluminate, 0.5-0.7 parts of a starch grafted acrylamide polymer, 0.1-0.3 parts of Halobacillus halophilus, 0.1-0.3 parts of Bacillus megaterium, 0.1-0.3 parts of Azotobacter vinelandii and 0.1-0.3 parts of Trichoderma Longibrachiatum.

The invention relates to the field of seed development and desertification control, in particular to a desertification control capsule, contents of the capsule and a preparing method of the contents.The contents of the desertification control capsule are prepared from, by weight, 40-70% of high-hydroscopicity bamboo fiber macromolecule polysaccharide, 5-10% of bacillus subtilis bacteria solution,5-10% of an azotobacter vinelandii bacteria solution, 8-20% of plant seeds capable of being planted in deserts and 8-20% of moso bamboo fiber fine powder. According to the desertification control capsule, the contents of the capsule and the preparing method of the contents, a circulation system is formed among the contents of the desertification control capsule, and the high-hydroscopicity bamboofiber macromolecule polysaccharide can quickly adsorb a large amount of moisture to provide sufficient moisture for germination and growth of microbes and seeds, and loosens soil to improve the gas permeability of the soil by adsorbing and releasing the moisture; bacillus subtilis and azotobacter vinelandii provide nutrients and growth promoting factors for the seed development, and metabolic products of the microbes and seeds are adsorbed and stored by the bamboo fiber macromolecule polysaccharide, so that the mutually-promoting good circulation system is formed among the contents.

A formulation for stabilizing soils is described comprising bacteria, enzymes and cations, wherein the bacteria preferably correspond to Azotobacter vinelandii and Acidithiobacillus ferrooxidans, the enzyme preferably corresponds to an alginase and the cations are preferably provided in the form of calcium chloride. A method for stabilizing soils; a method for preparing stabilized paths; and use of the soil-stabilizing composition are also described.

In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase-expressing bacteria capable of enzymatically synthesizing hydrocarbons and generating hydrogen and carbon monoxide, and for carbon dioxide and/or carbon monoxide recycling, and compositions (e.g., bioreactors and devices) for using them, and methods for making and using them. In alternative embodiments, the genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria used to practice embodiments provided herein include nitrogen-fixing diazotrophs such as nitrogen-fixing bacteria of the family Pseudomonadaceae, or the genus Azotobacter, including Azotobacter vinelandii, for the whole cell synthesis of hydrocarbons and generating hydrogen and carbon monoxide, and for the recycling of carbon dioxide and/or carbon monoxide.

The invention discloses a compound microbial agent for treating organic wastes of livestock and poultry, and relates to the technical field of microorganisms. The composite microbial agent comprises amicrobial agent and a solid carrier, wherein the microbial agent comprises bacillus megatherium, azotobacter vinelandii, bacillus mucilaginosus, thermococcus, aspergillus oryzae and trichoderma longibrachiatum; the solid carrier is a prolactan polysaccharide/halloysite composite microsphere. According to the compound microbial agent disclosed by the invention, a plurality of microorganisms with complementary and synergistic functions are mixed and inoculated to a solid carrier, so that the quantity and activity of the microorganisms can be increased, the mineralization degradation degree of the compound microbial agent on organic wastes in an aerobic composting process is enhanced, and the composting effect is improved. Meanwhile, emission of harmful gas can be reduced, and retention of nitrogen is promoted.

The invention belongs to the technical field of organic fertilizers for promoting crop growth, and particularly relates to a livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof. The preparation method comprises the following steps: culturing an Azotobacter vinelandii strain and an azotobacter chroococcum strain by adopting a culture medium, preparing a livestock and poultry manure fermented composted material by adopting a fermentation method, uniformly mixing the livestock and poultry manure composted material, an auxiliary material and alginate, and conducting drying, thereby obtaining the organic fertilizer. The organic fertilizer not only provides nutritional factors for growth of crops such as traditional Chinese medicinal materials, pasture and fruits and vegetables, but also has adhesion and slow release effects on nutritional ingredients of the organic fertilizer; and meanwhile, the rhizosphere micro-ecological environment of crops is improved, the soil fertility is improved, and the bacterial culture medium is improved, so that the reproductive capacity of Azotobacter vinelandii and azotobacter chroococcum is improved, the bacterial count is increased, and the yield of alginate is increased.