Compound microbial agent for treating organic wastes of livestock and poultry
A technology of compound microbial inoculants and microbial inoculants, applied in the field of microorganisms, can solve the problems such as hindering the effective utilization of livestock and poultry manure, the sustainable development of animal husbandry, the spread of diseases, environmental pollution, etc. The effect of good composting efficiency
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Embodiment 1
[0034] Preparation of pullulan polysaccharide / halloysite composite microspheres:
[0035] Weigh 1.8g halloysite powder and add it into 50mL deionized water, ultrasonically disperse for 2h to obtain a uniform suspension; weigh 0.9g pullulan polysaccharide and dissolve it in 50mL deionized water, and slowly add it to the halloysite suspension while stirring , continue to stir for 24h to obtain a halloysite solution modified by pullulan polysaccharide.
[0036]Add 35mL of paraffin, 25mL of petroleum ether, and 1mL of Tween-80 into a 250mL three-necked flask, and use electric stirring to stir slowly for 10min to make it evenly mixed. Increase the rotation speed to 1000rpm and stir for 20min to emulsify to form water-in-oil droplets, then add 10mL glutaraldehyde-saturated toluene solution and continue stirring for 90min. The whole reaction process is carried out in a constant temperature water bath at 25°C, and the total reaction time is 120min. After the end, the product was wash...
Embodiment 2
[0048] The preparation of pullulan polysaccharide / halloysite composite microspheres, matrix pretreatment, and liquid medium were the same as in Example 1.
[0049] Preparation of modified oyster shell powder:
[0050] Clean the silt and residual meat on the surface of oyster shells, and soak them in 1mol / L sodium hydroxide solution for 48 hours. Then rinse it with distilled water and put it in an oven for 2 hours at 105°C. Then the oyster shells are pulverized with a pulverizer, and the oyster shell powder that has been sieved through a 70 mesh sieve is used for subsequent use.
[0051] Take 100 g of the oyster shell powder obtained above and put it into a muffle furnace for roasting at 300° C. for 2 h, add 1 L of 0.1 mol / L oleic acid solution, and stir at 60° C. for 2 h to obtain surface-modified oyster shell powder for later use.
[0052] Preparation of a compound microbial bacterial agent for processing livestock and poultry organic waste:
[0053] (1) The bacterial clas...
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