Livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof
A technology of manure alginate and organic fertilizer, which is applied in the field of organic fertilizer to promote crop growth, can solve problems such as the difficulty in determining the interaction and mechanism, the inconspicuous effect of fertilizer and production increase, and the long time of bacterial culture, so as to maintain the root system of crops. The effect of microbial ecology, improvement of fecundity, and increase of bacterial count
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Embodiment 1
[0028] The preparation of embodiment 1 brown nitrogen-fixing bacteria alginate organic fertilizer
[0029] (1) Preparation of nitrogen-free liquid medium: first add 10 g of glucose, 0.1 g of potassium dihydrogen phosphate, 0.15 g of magnesium sulfate heptahydrate, 0.1 g of ferrous sulfate heptahydrate, and 0.005 g of molybdic acid dihydrate in 1 liter of deionized water Sodium and 0.2g sodium chloride are configured into a liquid medium, and then the soil leachate is added according to the ratio of the volume ratio of the soil leachate to the liquid medium of 1:10, and the pH is adjusted to 7.8.
[0030] (2) Preparation of brown nitrogen-fixing bacteria alginate: add 2 μl brown nitrogen-fixing bacteria strains in 20ml nitrogen-free liquid medium in proportion, culture on a shaker at 26°C for 48 hours, then transfer 2ml of the culture into a sterile centrifuge tube, Centrifuge at 6000rpm for 3min, wash the precipitate of the obtained strain twice with an equal volume of normal ...
Embodiment 2
[0034] Embodiment 2: the use of brown nitrogen-fixing bacteria alginate organic fertilizer in the cultivation of crops such as Chinese medicinal material Uncaria
[0035] The project test site of Guizhou Institute of Animal Husbandry and Veterinary Medicine is the main observation site, and three treatments are set up: livestock manure manure alginate organic fertilizer, livestock manure manure farmyard manure (cow and sheep manure processed by fermentation) and untreated manure Fertilization; each treatment is 100m of Chinese herbal medicine (Uncaria), pasture (annual ryegrass), feed and medicinal dual-purpose crop (golden buckwheat) 2 , sow in autumn or plant in spring, fertilize 2-3 times in the same period every year, microbial alginate organic fertilizer is 1000㎏ / 667 m 2 Dosage applied, livestock manure farmyard manure 1000㎏ / 667m 2 , other routine management is the same. Measure the height of the plant before flowering or heading, randomly measure 5 plants for each trea...
Embodiment 3
[0039] Embodiment 3: Cow dung polluted alginate organic fertilizer has influence on Chinese medicinal material whitening and output
[0040] (1) Configuration of nitrogen-free liquid medium for Azotobacter rotunda: first add 20g glucose, 0.2g potassium dihydrogen phosphate, 0.2g magnesium sulfate heptahydrate, 0.2g ferrous sulfate heptahydrate, 0.010g to 1 liter of deionized water Sodium molybdate dihydrate and 0.25g sodium chloride are configured into a liquid medium, and then the soil leachate is added at a ratio of 1:10 by volume of the soil leachate to the liquid medium to adjust the pH to 7.4.
[0041] (2) Preparation of Azotobacter rotundum alginate: Add 2 μl of Azotobacter rotundum strains in proportion to 20 ml of nitrogen-free liquid medium, and culture on a shaker at 30° C. for 36 hours; then take 200 μl of bacterial liquid and transfer it into 500 ml of liquid nitrogen-free In the Erlenmeyer flask of the culture medium, cultivate it on a shaker in an incubator at 28...
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