Livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof

A technology of manure alginate and organic fertilizer, which is applied in the field of organic fertilizer to promote crop growth, can solve problems such as the difficulty in determining the interaction and mechanism, the inconspicuous effect of fertilizer and production increase, and the long time of bacterial culture, so as to maintain the root system of crops. The effect of microbial ecology, improvement of fecundity, and increase of bacterial count

Inactive Publication Date: 2021-08-27
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional commercially available organic fertilizers are mainly added with modified slow-release agents to form high-speed slow-release organic fertilizers. There are more than 20 organic fertilizer components, and the interaction and mechanism of each component are difficult to determine, and modified antibacterial preservatives may affect Phosphorus solubilizing bacteria, potassium solubilizing bacteria, and nitrogen fixing bacteria have inhibitory effects, and some are even limited to use only on Yuqian, and the scope of use is limited. Brown nitrogen fixing bacteria and rotund nitrogen fixing bacteria alginate organic fertilizer are used as a crop growth promoting factor. It has not been used in crops such as Uncaria, white and golden buckwheat, garlic and annual ryegrass
[0005] The Chinese patent with the publication number CN102153387A discloses a p...

Method used

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  • Livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof
  • Livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof
  • Livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 brown nitrogen-fixing bacteria alginate organic fertilizer

[0029] (1) Preparation of nitrogen-free liquid medium: first add 10 g of glucose, 0.1 g of potassium dihydrogen phosphate, 0.15 g of magnesium sulfate heptahydrate, 0.1 g of ferrous sulfate heptahydrate, and 0.005 g of molybdic acid dihydrate in 1 liter of deionized water Sodium and 0.2g sodium chloride are configured into a liquid medium, and then the soil leachate is added according to the ratio of the volume ratio of the soil leachate to the liquid medium of 1:10, and the pH is adjusted to 7.8.

[0030] (2) Preparation of brown nitrogen-fixing bacteria alginate: add 2 μl brown nitrogen-fixing bacteria strains in 20ml nitrogen-free liquid medium in proportion, culture on a shaker at 26°C for 48 hours, then transfer 2ml of the culture into a sterile centrifuge tube, Centrifuge at 6000rpm for 3min, wash the precipitate of the obtained strain twice with an equal volume of normal ...

Embodiment 2

[0034] Embodiment 2: the use of brown nitrogen-fixing bacteria alginate organic fertilizer in the cultivation of crops such as Chinese medicinal material Uncaria

[0035] The project test site of Guizhou Institute of Animal Husbandry and Veterinary Medicine is the main observation site, and three treatments are set up: livestock manure manure alginate organic fertilizer, livestock manure manure farmyard manure (cow and sheep manure processed by fermentation) and untreated manure Fertilization; each treatment is 100m of Chinese herbal medicine (Uncaria), pasture (annual ryegrass), feed and medicinal dual-purpose crop (golden buckwheat) 2 , sow in autumn or plant in spring, fertilize 2-3 times in the same period every year, microbial alginate organic fertilizer is 1000㎏ / 667 m 2 Dosage applied, livestock manure farmyard manure 1000㎏ / 667m 2 , other routine management is the same. Measure the height of the plant before flowering or heading, randomly measure 5 plants for each trea...

Embodiment 3

[0039] Embodiment 3: Cow dung polluted alginate organic fertilizer has influence on Chinese medicinal material whitening and output

[0040] (1) Configuration of nitrogen-free liquid medium for Azotobacter rotunda: first add 20g glucose, 0.2g potassium dihydrogen phosphate, 0.2g magnesium sulfate heptahydrate, 0.2g ferrous sulfate heptahydrate, 0.010g to 1 liter of deionized water Sodium molybdate dihydrate and 0.25g sodium chloride are configured into a liquid medium, and then the soil leachate is added at a ratio of 1:10 by volume of the soil leachate to the liquid medium to adjust the pH to 7.4.

[0041] (2) Preparation of Azotobacter rotundum alginate: Add 2 μl of Azotobacter rotundum strains in proportion to 20 ml of nitrogen-free liquid medium, and culture on a shaker at 30° C. for 36 hours; then take 200 μl of bacterial liquid and transfer it into 500 ml of liquid nitrogen-free In the Erlenmeyer flask of the culture medium, cultivate it on a shaker in an incubator at 28...

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Abstract

The invention belongs to the technical field of organic fertilizers for promoting crop growth, and particularly relates to a livestock and poultry manure alginate organic fertilizer as well as preparation and application thereof. The preparation method comprises the following steps: culturing an Azotobacter vinelandii strain and an azotobacter chroococcum strain by adopting a culture medium, preparing a livestock and poultry manure fermented composted material by adopting a fermentation method, uniformly mixing the livestock and poultry manure composted material, an auxiliary material and alginate, and conducting drying, thereby obtaining the organic fertilizer. The organic fertilizer not only provides nutritional factors for growth of crops such as traditional Chinese medicinal materials, pasture and fruits and vegetables, but also has adhesion and slow release effects on nutritional ingredients of the organic fertilizer; and meanwhile, the rhizosphere micro-ecological environment of crops is improved, the soil fertility is improved, and the bacterial culture medium is improved, so that the reproductive capacity of Azotobacter vinelandii and azotobacter chroococcum is improved, the bacterial count is increased, and the yield of alginate is increased.

Description

technical field [0001] The invention relates to the technical field of an organic fertilizer for promoting crop growth, in particular to the preparation and application of a nitrogen-fixing bacteria brown and nitrogen-fixing bacteria ruthenus alginate organic fertilizer for promoting crop growth. Background technique [0002] Brown nitrogen-fixing bacteria and rufous nitrogen-fixing bacteria belong to the genus of autotrophic nitrogen-fixing bacteria, which are beneficial microorganisms that can synthesize biological nitrogenase and fix free nitrogen in the air, improve soil fertility, maintain the microbial ecology of crop roots, and promote the growth of non-legume plants, but nitrogen fixation The use of bacteria is affected and limited by conditions such as strain storage, transportation, and climate; alginate is a functional oligosaccharide, which is usually extracted from natural marine brown algae, and can also be obtained from bacteria such as brown nitrogen-fixing ba...

Claims

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Application Information

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IPC IPC(8): C05G3/40C05G3/80C05F17/20C05F17/80
CPCC05G3/40C05G3/80C05B1/02C05F17/20C05F17/80Y02W30/40
Inventor 杨茂生唐远江杨莉杜春林蒲玲张涛张杨子田宇杰梁应林杨丽娟
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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