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Preparation method of liquid composite bacterial strain

A technology of compound strains and liquid strains, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of single structure, difficult to meet the requirements of producing nutritionally balanced compound fertilizers, etc., and achieve low comprehensive cost. , storage and cultivation are easy to operate, and the effect of low investment

Inactive Publication Date: 2014-03-05
SHENZHEN XINHUA NANFANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for preparing liquid composite strains, which overcomes the defects that the existing liquid composite strains have a single structure and are difficult to meet the requirements for producing nutritionally balanced compound fertilizers

Method used

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with embodiment.

[0026] In the preparation method of the liquid composite bacteria of the present invention, the prepared liquid composite bacteria include nitrogen-fixing bacteria, potassium-dissolving bacteria and antibiotic bacteria. in:

[0027] The nitrogen-fixing bacteria is one of Rhizobium, Azotobacter rufosa, Agrobacterium tumefaciens C58 / PGV3850 strain, Rhizobium hainanensis 166, Azospirillum Yu-62, Azotobacter K1ebsie11a p1antico1a19-1 or Azotobacter brown Az230 .

[0028] The potassium-solubilizing bacteria is one of the silicate bacteria NBT strain, the potassium bacteria JF88 strain or the silicate bacteria G10 strain.

[0029] The antibiotic is one of Streptomyces, Penicillium, Bacillus polymyxa or 5406 antibiotics.

[0030] The preparation method of liquid composite strain of the present invention is as follows:

[0031] First, select one kind of bacteria from the above-mentioned...

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PUM

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Abstract

The invention provides a preparation method of a liquid composite bacterial strain. The bacterial strain comprises azotobacteria, potassium decomposing bacterium, and antibiotic bacterium; wherein the azotobacteria is a bacterium selected from the following bacteria: rhizobium, azotobacter chroococcum, C58 / PGV3850 strain of agrobacterium tumefaciens, Hainan rapid growing rhizobium 166, azospirillum Yu-62, associative nitrogen-fixing bacteria Klebsiella planticola 19-1 and azotobacter vinelandii Az230; the potassium decomposing bacterium is a bacterium selected from the following bacteria: NBT strain of silicate bacteria, JF88 stain of potassium bacteria, and G10 strain of silicate bacteria; the antibiotic bacterium is a bacterium selected form the following bacteria: streptomycete, penicillium, bacillus polymyxa and 5406 antibiotic bacterium. The preparation method comprises the following steps: separately selecting one bacterium from the azotobacteria group, the potassium decomposing bacterium group and the antibiotic bacterium group mentioned above; individually preparing corresponding bacterial strain culture mediums, wherein the pH values of the culture mediums are in a range of 6 to 8.5; then individually subjecting the three culture mediums to conventional processes of packaging in pots, sterilizing, cooling, inoculating, and culturing so as to obtain liquid bacterial strains; then mixing the three liquid bacterial strains, wherein the weight ratio of the azotobacteria to the potassium decomposing bacterium to the antibiotic bacterium is 1:1:(0.025 to 0.05). The preparation method meets the requirement of production of nutritionally-balanced compound feed, and has the advantages of little investment, easy mastery, easiness, and low cost.

Description

technical field [0001] The invention relates to a preparation method for liquid composite bacteria, which can be used for the preparation of liquid fertilizer. Background technique [0002] Existing liquid quick-acting compound fertilizers are inorganic fertilizers that are specifically mixed with chemical fertilizers such as nitrogen, phosphorus, and potassium. Because chemical fertilizers have defects such as damaging soil fertility and causing soil compaction, people gradually focus on natural fertilizers that are environmentally friendly, efficient, harmless, and can enhance soil fertility, especially natural fertilizers that have been decomposed. [0003] Liquid compound strains are necessary for the production of natural liquid quick-acting compound fertilizers. The existing liquid compound strains have a single structure and are difficult to meet the requirements for producing nutritionally balanced compound fertilizers. Contents of the invention [0004] The techn...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12R1/065C12R1/41C12R1/465C12R1/12C12R1/80C12R1/01
Inventor 方小红吴峰
Owner SHENZHEN XINHUA NANFANG BIOTECH
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