Sheep placenta amino acid and low molecular weight polypeptide, and preparation method for sheep placenta amino acid and low molecular weight polypeptide

An amino acid, sheep placenta technology, applied in the direction of fermentation, can solve the problem of losing the meaning of using the placenta, the content of polypeptides and amino acids is low, and achieve the effect of increasing sperm production, improving infertility, and delaying stretch marks.

Inactive Publication Date: 2014-11-26
HUZHOU KANGHAISI BIOLOGICAL TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we believe that the above two preparations have their own disadvantages. The former destroys the natural active substances in the placenta during the hydrolysis process and loses the meaning of using the placenta; although the latter has a variety of natural active substances, the content of polypeptides and amino acids is very small, such as It can integrate the two, not only retain the natural active substances in the placenta, but also increase the content of peptides and amino acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of sheep placenta amino acid and low-molecular-weight polypeptide, the method comprises the following steps:

[0030] 1) Pretreatment of sheep embryos and sheep placenta raw materials: take sheep embryos and sheep placenta aged 2-3 months as raw materials, wash and remove impurities, and crush them into coarse powder at a low temperature of 0-5°C.

[0031] 2) Superfine pulverization of sheep embryos and sheep placenta, cell wall breaking: freeze the coarse powder of sheep embryos and sheep placenta at low temperature (-40℃~-45℃) for 2 hours, then ultrafine pulverization at low temperature (-20℃) (WZJ100 medicinal super micro pulverizer), the cell wall is broken to reach a particle size of 0.1-10μm.

[0032] 3) Extraction and enzymatic hydrolysis of sheep embryo and sheep placenta: add 6 times of distilled water to extract the sheep embryo and sheep placenta serum after ultrafine grinding and cell wall breaking, then filter to obtain filtrate A and p...

Embodiment 2

[0046] A method for preparing sheep placenta amino acids and low-molecular-weight polypeptides, except that papain is used for enzymatic hydrolysis in step 3), and other technical solutions are as described in Example 1.

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Abstract

The invention relates to a method for preparing sheep embryo and sheep placenta biological products, in particular to a method for preparing sheep placenta amino acid and low molecular weight polypeptide. The method comprises the following steps of: 1) pretreating sheep embryo and sheep placenta raw materials; 2) performing ultra-fine pulverization and cell wall breaking of sheep embryo and sheep placenta; 3) performing extraction and enzymolysis on the sheep embryo and the sheep placenta; and 4) performing fractional refining on a stock solution of the sheep embryo and the sheep placenta obtained in the step 3) by using a sephadex chromatography method according to molecular weight to obtain the sheep placenta amino acid and the low molecular weight polypeptide. By the sheep placenta amino acid and the low molecular weight polypeptide prepared by the method, active materials of the sheep embryo and the sheep placenta are retained furthest and are easily absorbed by a human body comprehensively; and the process is advanced and reasonable, and the quality is controllable.

Description

technical field [0001] The invention relates to a sheep embryo and sheep placenta biological product and a preparation method thereof. Background technique [0002] Our country has a long history of using various organs, tissues and metabolites of animals for disease treatment, disease prevention and medical care. There is a complete pharmacy classic - "Shen Nong's Materia Medica", which includes bezoar, rhino horn, velvet antler, donkey-hide gelatin and other animals. Drug records. It shows that our country has already used animal drugs to process, extract, and refine in pharmaceutical technology to form relatively pure pharmaceuticals. [0003] Placenta (Placenta), also known as "Ziheche", is a commonly used traditional Chinese medicine. Modern medical research shows that: placenta contains a variety of immunoglobulins, active peptides and other biologically active substances, amino acids, minerals and other components (collectively referred to as live cells). A regulato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12P21/06
Inventor 姜雪琪姜艳
Owner HUZHOU KANGHAISI BIOLOGICAL TECH
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